Human Cell cycle checkpoint protein RAD1, RAD1 ELISA KIT Product Name:Human Cell cycle checkpoint protein RAD1, RAD1 ELISA KIT
Packing:96T
Catalog No.:ELI-14922h
Gene Name:RAD1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cell cycle checkpoint protein RAD1(RAD1
Alternative Name:RAD1,Cell cycle checkpoint protein RAD1(RAD1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cell cycle checkpoint protein RAD1, RAD1 ELISA KIT allows for the in vitro quantitative determination of Cell cycle checkpoint protein RAD1(RAD1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cell cycle checkpoint protein RAD1, RAD1 ELISA KIT has been pre-coated with an Cell cycle checkpoint protein RAD1(RAD1 antibody specific to Cell cycle checkpoint protein RAD1(RAD1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cell cycle checkpoint protein RAD1(RAD1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cell cycle checkpoint protein RAD1(RAD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cell cycle checkpoint protein RAD1(RAD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!