Human Ellis- van Creveld syndrome protein, EVC ELISA KIT Product Name:Human Ellis- van Creveld syndrome protein, EVC ELISA KIT
Packing:96T
Catalog No.:ELI-08328h
Gene Name:EVC
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Ellis-van Creveld syndrome protein(EVC
Alternative Name:EVC,Ellis-van Creveld syndrome protein(EVC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ellis- van Creveld syndrome protein, EVC ELISA KIT allows for the in vitro quantitative determination of Ellis-van Creveld syndrome protein(EVC concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ellis- van Creveld syndrome protein, EVC ELISA KIT has been pre-coated with an Ellis-van Creveld syndrome protein(EVC antibody specific to Ellis-van Creveld syndrome protein(EVC .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ellis-van Creveld syndrome protein(EVC and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ellis-van Creveld syndrome protein(EVC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ellis-van Creveld syndrome protein(EVC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!