Human Tryptase alpha/beta- 1, TPSAB1 ELISA KIT

Human Tryptase alpha /beta- 1, TPSAB1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

TPS,Tpsab1,Tryptase,tryptase alpha/beta 1; tryptase skin; tryptase beta 1; tryptase alpha/beta-1; Mcpt7; TPSB1; rMCP-7; MMCP-7; mast cell protease 7

Search name

Human TPS ELISA KIT,Human Tpsab1 ELISA KIT ,Human Tryptase ELISA KIT ,Human tryptase alpha/beta 1 ELISA KIT ,Human tryptase skin ELISA KIT ,Human tryptase beta 1 ELISA KIT ,Human tryptase alpha/beta-1 ELISA KIT ,Human Mcpt7 ELISA KIT ,Human TPSB1 ELISA KIT ,Human rMCP-7 ELISA KIT ,Human MMCP-7 ELISA KIT ,Human mast cell protease 7 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination ofhumanTryptase-1concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptase-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forTryptase-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tryptase-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tryptase-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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