Human Vitronectin, VTN ELISA KIT

Human Vitronectin, VTN ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Vitronectin,Serum-spreading factor,Glycoprotein 66,S-protein, vitronectin,V75; VNT; S-protein; complement S-protein; epibolin; serum spreading factor; serum-spreading factor; somatomedin B

Search name

Human Vitronectin ELISA Kit, Human VNT ELISA Kit, Human Serum-spreading factor ELISA Kit, Human Glycoprotein 66 ELISA

Kit, Human S-protein ELISA Kit, Human V75 ELISA Kit, Human complement S-protein ELISA Kit, Human epibolin ELISA Kit,

Human somatomedin B ELISA Kit, Human serum spreading factor ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human vitronectin,VN concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to VN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for VN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain VN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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