pEarleyGate 301/ pEarleyGate 301质粒/ pEarleyGate 301 Plasmid_详细资料

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货号： PVT11165 CAS号：无供应商： LSM Bio 数量：货源充足英文名： pEarleyGate 301 保质期： 6month 保存条件：负20摄氏度规格： 2ug

pEarleyGate 301

Catalog No. PVT11165
Packing 2ug

pEarleyGate 301 Information

Function plant expression plasmid

Terminator: OCS Terminator

Plasmid tagging: C-HA

Prokaryotic resistance: kanamycin Kan

Screening marker: Bar

Clone strain: Escherichia coli DB3.1

Culture conditions: 37 C

pEarleyGate 301 Description

PEarleyGate 301 (C-HA) is a Gateway expression system for plant cells. pEarleyGate 301 allows for rapid recombinational cloning of DNA from Gateway entry vectors to be epitope tagged with HA tag at the C-terminus of the encoded protein. This vector allows for expression of genes from a promoter of choice rather than from the enhanced 35S promoter (see pEarleyGate 101-105 or 201-204).

pEarleyGate 301 Sequence

LOCUS Exported 10379 bp ds-DNA circular SYN 18-DEC-2017

DEFINITION Gateway(R)-compatible promoterless plant transformation vector with

a C-terminal HA tag.

KEYWORDS pEarleyGate 301

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 10379)

AUTHORS Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.

TITLE Gateway-compatible vectors for plant functional genomics and

proteomics.

JOURNAL Plant J. 2006;45:616-29.

PUBMED 16441352

REFERENCE 2 (bases 1 to 10379)

AUTHORS Pikaard Lab

TITLE Direct Submission

COMMENT The sequence was corrected by inserting a G at position 6487.

FEATURES Location/Qualifiers

source 1..10379

/organism="synthetic DNA construct"

/lab_host="Plant Cells"

/mol_type="other DNA"

misc_feature 3..27

/label=LB T-DNA repeat

/note="left border repeat from nopaline C58 T-DNA"

terminator 113..365

/label=MAS terminator

/note="mannopine synthase terminator"

CDS complement(375..926)

/codon_start=1

/gene="Streptomyces hygroscopicus bar"

/product="phosphinothricin acetyltransferase"

/label=Bar

/note="confers resistance to bialophos or phosphinothricin"

/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE

WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS

TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF

WQLDFSLPVPPRPVLPVTEI"

promoter complement(932..1312)

/label=MAS promoter

/note="mannopine synthase promoter (Velten et al., 1984)"

protein_bind 1364..1483

/gene="mutant version of attR"

/label=attR1

/bound_moiety="LR Clonase(TM)"

/note="recombination site for the Gateway(R) LR reaction"

promoter 1513..1543

/label=lac UV5 promoter

/note="E. coli lac promoter with an ""up"" mutation"

CDS 1597..2256

/codon_start=1

/gene="cat"

/product="chloramphenicol acetyltransferase"

/label=Chl

/note="confers resistance to chloramphenicol"

/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL

KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS

LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM

DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"

CDS 2598..2903

/codon_start=1

/gene="ccdB"

/product="CcdB, a bacterial toxin that poisons DNA gyrase"

/label=ccdB

/note="Plasmids containing the ccdB gene cannot be

propagated in standard E. coli strains."

/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK

VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"

protein_bind complement(2944..3068)

/gene="mutant version of attR"

/label=attR2

/bound_moiety="LR Clonase(TM)"

/note="recombination site for the Gateway(R) LR reaction"

CDS 3070..3096

/codon_start=1

/product="HA (human influenza hemagglutinin) epitope tag"

/label=HA

/translation="YPYDVPDYA"

terminator 3150..3857

/label=OCS terminator

/note="octopine synthase terminator"

misc_feature 4101..4125

/label=RB T-DNA repeat

/note="right border repeat from nopaline C58 T-DNA"

CDS 5425..6054

/codon_start=1

/product="stability protein from Pseudomonas plasmid pVS1"

/label=pVS1 StaA

/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR

DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP

VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI

LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

CDS 6483..7556

/codon_start=1

/product="replication protein from Pseudomonas plasmid

pVS1"

/label=pVS1 RepA

/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW

QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS

KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP

GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE

ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR

LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI

LVMRYRNLIEGEASAGS"

rep_origin 7622..7816

温馨提示：不可用于临床ZL。

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