pRSV-Rev Catalog No. PVT11130
Packing 2ug
pRSV-Rev Information
Promoter: RSV
Replicator: pUC ori, F1 ori
Plasmid classification: virus series, lentivirus packaging carrier
Plasmid size: 4180bp
Prokaryotic resistance: Amp
Cloned strain: Stbl3
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, instantaneous expression
5'sequencing primers: M13R:CAGGAAACAGCTATGACC
3'sequencing primers: primers designed according to sequence
pRSV-Rev Description
The HIV-1 Rev of the second and third exons added to the Rev cDNA expression plasmid is controlled by the transcription of the RSV U3 promoter. Note that most trono lab lentivirus vectors can only use the second generation packaging system.
pRSV-Rev Sequence
LOCUS Exported 4180 bp ds-DNA circular SYN 01-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4180)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, September 1, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..4180
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 1024..1612
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 1900..1921
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1936..1966
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 1974..1990
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1998..2014
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2159..2420
/note="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
primer_bind complement(2983..2999)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 3331..3786
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4068..4172
/gene="bla"
/note="AmpR promoter"
CDS 4173..853
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
61 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
121 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
181 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
241 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
301 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
361 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
421 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
481 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
541 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
601 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
661 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
721 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
781 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
841 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
1021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
1081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
1141 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
1201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
1261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
1321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
1381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
1441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
1501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
1561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
1621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
1681 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
1741 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
1801 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
1861 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
1921 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
1981 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga tgtacgggcc
2041 agatatacgc gtatctgagg ggactagggt gtgtttaggc gaaaagcggg gcttcggttg
2101 tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa
2161 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
温馨提示：不可用于临床ZL。
pRSV- Rev/ pRSV- Rev质粒/ pRSV- Rev Plasmid
Addgene质粒-pPACKH1-REV慢病毒类质粒
百欧博伟 质粒pPACKH1-REV
第三代慢病毒包装质粒 pRSV-Rev pMDLg/pRRE pMD2.G q1822235623
P0700/pRSV-Rev慢病毒包装质粒
pRSV-Rev质粒载体
pRSV-Rev慢病毒包装质粒
pRSV-Rev慢病毒包装质粒
第三代慢病毒包装质粒 pRSV-Rev pMDLg/pRRE pMD2.G
pRSV-Rev慢病毒包装质粒
