基本信息
| 启动子: | RSV |
| 复制子: | pUC ori,f1 ori |
| 质粒分类: | 病毒系列，慢病毒包装载体 |
| 质粒大小: | 4180bp |
| 原核抗性: | Amp |
| 克隆菌株: | Stbl3 |
| 培养条件: | 37℃，有氧 LB |
| 表达宿主: | 哺乳细胞 |
| 诱导方式: | 无须诱导，瞬时表达 |
| 5'测序引物: | M13R:CAGGAAACAGCTATGACC |
| 3'测序引物: | 根据序列设计引物 |
质粒简介
Rev cDNA表达质粒中，加入的第二和第三外显子的HIV-1 Rev是RSV U3启动子的转录控制下。请注意，大多数trono实验室慢病毒载体只能使用第二代包装系统。
质粒图谱
质粒序列
LOCUS Exported 4180 bp ds-DNA circular SYN 01-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4180)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, September 1, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..4180
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 1024..1612
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 1900..1921
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1936..1966
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 1974..1990
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1998..2014
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2159..2420
/note="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
primer_bind complement(2983..2999)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 3331..3786
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4068..4172
/gene="bla"
/note="AmpR promoter"
CDS 4173..853
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
61 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
121 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
181 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
241 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
301 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
361 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
421 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
481 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
541 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
601 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
661 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
721 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
781 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
841 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
1021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
1081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
1141 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
1201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
1261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
1321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
1381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
1441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
1501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
1561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
1621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
1681 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
1741 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
1801 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
1861 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
1921 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
1981 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga tgtacgggcc
2041 agatatacgc gtatctgagg ggactagggt gtgtttaggc gaaaagcggg gcttcggttg
2101 tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa
2161 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
2221 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
2281 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattcc
2341 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacgcc atttgaccat
2401 tcaccacatt ggtgtgcacc tccaagctcg agctcgttta gtgaaccgtc agatcgcctg
2461 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc
2521 ctcgaagcta gtcgattagg catctcctat ggcaggaaga agcggagaca gcgacgaaga
2581 cctcctcaag gcagtcagac tcatcaagtt tctctatcaa agcaacccac ctcccaatcc
2641 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
2701 gatccattcg attagtgaac ggatccttag cacttatctg ggacgatctg cggagcctgt
2761 gcctcttcag ctaccaccgc ttgagagact tactcttgat tgtaacgagg attgtggaac
2821 ttctgggacg cagggggtgg gaagccctca aatattggtg gaatctccta caatattgga
2881 gtcaggagct aaagaatagt gctgttagct tgctcaatgc cacagctata gcagtagctg
2941 aggggacaga tagggttata gaagtagtac aagaagcttg gcactggccg tcgttttaca
3001 acgtcgtgat ctgagcctgg gagatctctg gctaactagg gaacccactg cttaagcctc
3061 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
3121 actagagatc aggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
3181 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
3241 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
3301 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
3361 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
3421 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
3481 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
3541 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
3601 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
3661 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
3721 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
3781 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
3841 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
3901 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
3961 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
4021 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
4081 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
4141 aaatgcttca ataatattga aaaaggaaga gtatgagtat
温馨提示：不可用于临床ZL。
