pLenti CMV V5- LUC Blast (w567- 1)/ pLenti CMV V5- LUC Blast (w567- 1)质粒/ pLenti CMV V5- LUC Blast (w567- 1) Plasmid_详细资料

产品信息

货号： PVT11097 CAS号：无供应商： LSM Bio 数量：货源充足英文名： pLenti CMV V5- LUC Blast (w567- 1) 保质期： 6month 保存条件：负20摄氏度规格： 2ug

pLenti CMV V5-LUC Blast (w567-1)

Catalog No. PVT11097
Packing 2ug

pLenti CMV V5-LUC Blast (w567-1) Informaiton

Promoter: CMV promoter

Replicator: Ori, F1 ori, SV40 ori

Terminator: WPRE

Plasmid classification: virus series plasmid; lentiviral plasmid; virus expression plasmid.

Plasmid size: 9463bp

Plasmid tagging: N-V5

Prokaryotic resistance: ampicillin Amp

Screening marker: magnaporin Blast

Clone strain: Escherichia coli Stbl3

Culture conditions: 37 C, aerobic, LB

Expression host: mammalian cells such as 293T

pLenti CMV V5-LUC Blast (w567-1) Description

3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, Blast.

pLenti CMV V5-LUC Blast (w567-1) Reference

A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells .

pLenti CMV V5-LUC Blast (w567-1) Sequence

LOCUS Exported 9463 bp ds-DNA circular SYN 17-鍏湀-2017

KEYWORDS pLenti CMV V5-LUC Blast (w567-1)

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 9463)

FEATURES Location/Qualifiers

source 1..9463

/organism="synthetic DNA construct"

/mol_type="other DNA"

promoter 27..131

/gene="bla"

/note="AmpR promoter"

CDS 132..992

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/note="AmpR"

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

rep_origin 1163..1751

/direction=RIGHT

/note="ori"

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

LTR 2448..2628

/note="5' LTR (truncated)"

/note="truncated 5' long terminal repeat (LTR) from HIV-1"

misc_feature 2672..2797

/note="HIV-1 Psi"

/note="packaging signal of human immunodeficiency virus

type 1"

misc_feature 3290..3523

/note="RRE"

/note="The Rev response element (RRE) of HIV-1 allows for

Rev-dependent mRNA export from the nucleus to the

cytoplasm."

misc_feature 4012..4129

/note="cPPT/CTS"

/note="central polypurine tract and central termination

sequence of HIV-1"

enhancer 4152..4455

/note="CMV enhancer"

/note="human cytomegalovirus immediate early enhancer"

promoter 4456..4659

/note="CMV promoter"

/note="human cytomegalovirus (CMV) immediate early

promoter"

protein_bind 4764..4788

/gene="mutant version of attB"

/bound_moiety="BP Clonase(TM)"

/note="attB1"

/note="recombination site for the Gateway(R) BP reaction"

CDS 4800..4841

/codon_start=1

/product="epitope tag from simian virus 5"

/note="V5"

/translation="GKPIPNPLLGLDST"

CDS 4917..6569

/codon_start=1

/gene="luc+"

/product="firefly luciferase"

/EC_number="

"

/note="luciferase"

/note="enhanced luc+ version of the luciferase gene"

/protein_id="

"

/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA

HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP

ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS

MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA

RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK

IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY

GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS

GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL

LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV

FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"

protein_bind complement(6576..6600)

/gene="mutant version of attB"

/bound_moiety="BP Clonase(TM)"

/note="attB2"

/note="recombination site for the Gateway(R) BP reaction"

misc_feature 6631..7219

/note="WPRE"

/note="woodchuck hepatitis virus posttranscriptional

regulatory element"

promoter 7275..7605

/note="SV40 promoter"

/note="SV40 enhancer and early promoter"

rep_origin 7456..7591

/note="SV40 ori"

/note="SV40 origin of replication"

promoter 7653..7700

/note="EM7 promoter"

/note="synthetic bacterial promoter "

CDS 7719..8117

/codon_start=1

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