pRSETB-SFGFP PVT10616 2ug
pRSETB-SFGFP Description
Prokaryotic resistance: ampicillin Amp
Clone strain: Escherichia coli DH5 alpha
Culture conditions: 37 LB, medium.
PRSETB-SFGFP is a green fluorescent expression plasmid of large intestine.
pRSETB-SFGFP Sequence
LOCUS Exported 3571 bp ds-DNA circular SYN 26-JUN-2017
DEFINITION synthetic circular DNA
KEYWORDS pRSETB-SFGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3571)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3571
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 20..38
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 112..129
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 133..165
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 169..192
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/note="Xpress(TM) tag"
/translation="DLYDDDDK"
CDS 199..918
/codon_start=1
/note="SFGFP"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
terminator 986..1033
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 1130..1585
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1611..1715
/gene="bla"
/note="AmpR promoter"
CDS 1716..2576
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2747..3335
/direction=RIGHT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
181 gatgacgata aggatccgat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
241 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgag aggcgagggc
301 gagggcgatg ccaccaatgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
361 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
421 taccccgacc acatgaagcg ccacgacttc ttcaagtccg ccatgcccga aggctacgtc
481 caggagcgca ccatcagttt caaggacgac ggcacataca agacccgcgc cgaggtgaag
541 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
601 ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaacgt ctatatcacg
661 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa cgtggaggac
721 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
781 ctgctgcccg acaaccacta cctgagcacc cagtccgtgc tgagcaaaga ccccaacgag
841 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tcacggcatg
901 gacgagctgt acaagtaagc ttgatccggc tgctaacaaa gcccgaaagg aagctgagtt
961 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
1021 gaggggtttt ttgctgaaag gaggaactat atccggatct ggcgtaatag cgaagaggcc
1081 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
1141 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
1201 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
1261 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
1321 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
1381 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
1441 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
1501 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
1561 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
1621 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
1681 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
1741 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
1801 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
1861 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
1921 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
1981 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
2041 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
2101 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
2161 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
2221 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
温馨提示：不可用于临床ZL。
pRSETB- SFGFP/ pRSETB- SFGFP质粒/ pRSETB- SFGFP Plasmid
pRSETB-Clover/ pRSETB-Clover质粒
P2142/pTD103luxI_sfGFP质粒
pTD103luxI_sfGFP质粒
pTD103luxI_sfGFP质粒
pTD103luxI-sfGFP质粒
P1319/pRSETB-SFGFP大肠荧光质粒
沪公网安备 31011502008050号
