pCMV- dR8.91/ pCMV- dR8.91质粒/ pCMV- dR8.91 Plasmid_详细资料

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货号： PVT2323 CAS号：无供应商： LSM Bio 数量：货源充足英文名： pCMV- dR8.91 保质期： 6month 保存条件：负20摄氏度规格： 2ug

pCMV-dR8.91
Search name
pCMV-dR8.91,Plasmid pCMV-dR8.91,pCMV-dR8.91 vector
Bacterial Resistance:
Growth Strain:

Use:Lentiviral

Promoter: CMV, SP6
Replicator: pUC, ori, SV40, ori
Plasmid classification: viral series, lentivirus packaging vector
Plasmid size: 12150bp
Prokaryotic resistance: Amp
Clone strain: Stbl3
Culture conditions: 37 DEG C, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, transient expression
Primers for 5'sequencing: SP6:ATTTAGGTGACACTATAGAA
Primers for 3'sequencing: primers were designed according to sequences

Sequences

LOCUS Exported 12150 bp ds-DNA circular SYN 27-AUG-2016

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS Untitled 31

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 12150)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Saturday, August 27, 2016 from SnapGene Viewer 3.1.4

FEATURES Location/Qualifiers

source 1..12150

/organism="synthetic DNA construct"

/mol_type="other DNA"

promoter 377..580

/note="CMV promoter"

/note="human cytomegalovirus (CMV) immediate early

promoter"

CDS 855..2357

/codon_start=1

/gene="gag"

/product="gag protein from human immunodeficiency virus 1"

/note="HIV-1 gag"

/translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA

VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI

EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA

FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP

IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS

ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG

ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG

HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP

PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"

CDS 2150..5161

/codon_start=1

/gene="pol"

/product="pol protein from human immunodeficiency virus 1"

/note="HIV-1 pol"

/translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG

ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG

GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV

PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD

STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK

YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD

DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI

VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL

AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT

NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP

LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ

AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH

KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA

SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET

AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK

ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK

QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ

MAGDDCVASRQDED"

misc_feature 4846..4963

/note="cPPT/CTS"

/note="central polypurine tract and central termination

sequence of HIV-1"

misc_feature 5735..5968

/note="RRE"

/note="The Rev response element (RRE) of HIV-1 allows for

Rev-dependent mRNA export from the nucleus to the

cytoplasm."

promoter complement(7140..7158)

/note="SP6 promoter"

/note="promoter for bacteriophage SP6 RNA polymerase"

promoter 7946..8050

/gene="bla"

/note="AmpR promoter"

CDS 8051..8911

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/note="AmpR"

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

rep_origin 9082..9670

/direction=RIGHT

/note="ori"

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

promoter 9916..10245

/note="SV40 promoter"

/note="SV40 enhancer and early promoter"

rep_origin 10096..10231

/note="SV40 ori"

/note="SV40 origin of replication"

intron 11478..11543

/note="small t intron"

/note="simian virus 40 (SV40) small t antigen intron"

CDS 11673..11693

/codon_start=1

/product="nuclear localization signal of SV40 large T

antigen"

/note="SV40 NLS"

/translation="PKKKRKV"

enhancer 12147..376

/note="CMV enhancer"

/note="human cytomegalovirus immediate early enhancer"

ORIGIN

1 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata

61 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga

121 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt

181 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt

241 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca

301 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt

361 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt

421 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca

481 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg

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