Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
Measured by its binding ability in a functional ELISA. Immobilized Human Vitronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV & ITGB5 Heterodimer Protein (Cat. No. IT5-H82Wa) with a linear range of 0.01-0.156 μg/mL (QC tested). Measured by its binding ability in a functional ELISA. Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV & ITGB5 Heterodimer Protein (Cat. No. IT5-H82Wa) with a linear range of 0.156-5 μg/mL (Routinely tested).

产品背景:
Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.

产品描述:
Biotinylated Human ITGAV & ITGB5 Heterodimer Protein, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 114.7 kDa (ITGAV) and 81.9 kDa (ITGB5). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 140-150 kDa (ITGAV) and 90-98 kDa (ITGB5) respectively due to glycosylation.
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