品名 | anti- beta actin antibody |
货号 | FNab00869 |
规格 | 100µg |
状态 | liquid |
纯化方法 | Immunogen affinity purified |
纯度 | ≥95% as determined by SDS-PAGE |
宿主 | Rabbit |
亚型 | IgG |
存储 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 24 months(Avoid repeated freeze / thaw cycles.) |
背景介绍 | Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha(ACTC1, cardiac muscle 1), alpha 1(ACTA1, skeletal muscle) and 2(ACTA2, aortic smooth muscle), beta(ACTB), gamma 1(ACTG1) and 2(ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2(rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. |
免疫原 | actin, beta |
别称 | ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, β actin |
分子量 | 42 kDa |
Uniprot ID | P60709 |
适用种属 | Human, Mouse, Rat |
验证应用 | ELISA, IF, WB, IHC |
建议稀释比例 | WB: 1:500-1:2000; IHC: 1:20-1:500; IF: 1:10-1:100 |
图片 | | Immunohistochemistry of paraffin-embedded human heart using FNab00869(ACTB antibody) at dilution of 1:100 |
| C6 cells were subjected to SDS PAGE followed by western blot with FNab00869(beta actin Antibody) at dilution of 1:2000 |
注意事项 | beta actin antibody|beta actin抗体仅供科研使用 |
产品链接 | https://www.fn-test.cn/product/FNab00869/ |