| Anti β-Actin Monoclonal Antibody | 说明书下载 | Calatog Number: | LT0427 | Lot Number: | 411005100207 | Isotype: | Mouse IgG2b | Clonality: | BA3R | Quantity: | 50 μg | Immunogen: | β-Actin N-terminal peptide-KLH conjugates | Specificity: | Recognizes native and denatured forms of β-Actin (~42kDa) | Cross Reactivity: | β-Actin from human, mouse, rat, rabbit, and chicken. β-Actin from other species may also be detectable | Purification: | Protein A affinity chromatography from mouse ascites fluid | Applications: | Dot, ELISA, IS, WB | Working Condition: | WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test | Storage: | Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks | Images: | Fig. 1:1000 (1μg/mL) Ab dilution used in WB of 20μg/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4) | |