| Anti GST Monoclonal Antibody | 说明书下载 | Calatog Number: | LT0423 | Lot Number: | 411005100205 | Isotype: | Mouse IgG2a | Clonality: | GST.B6 | Quantity: | 50 μg | Immunogen: | GST protein | Specificity: | Recognizes native and denatured forms of purified GST or GST fusion proteins | Purification: | Protein A affinity chromatography from mouse ascites fluid | Applications: | Dot, ELISA, IS, IP, WB | Sensitivity: | WB (with ECL): 10-50ng of purified GST or GST fusion proteins | Working Condition: | WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test | Storage: | Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks | Images: | Fig. 1:5000 (0.2μg/mL) Ab dilution probed against BL-21 bacteria lysate expressing the GST protein | |