重组链球菌蛋白质G琼脂胶_详细资料

产品信息

保存条件： -20 供应商： LifeTein 数量： 5 英文名： Recombinant Protein G Agarose 保质期： 3 规格： 2 mL

Catalog Number:

LT12015

Packing Details:

2 mL resin, crosslinked 6% beaded agarose supplied as 50% slurry (e.g., 2mL of settled resin is equivalent to 4mL of 50% slurry) in 0.02% sodium azide

Binding Capacity:

20-25 mg human IgG per mL of settled resin

Support pH Stability:

2-14 (short term); 3-13 (long term)

Average Particle Size:

45 to 165 microns

Exclusion Limit:

10,000 to 4,000,000 daltons

Maximum Volumetric Flow Rate:

approx. 1mL/minute (for 1cm diameter column); Maximum Linear Velocity: 30cm per hour; Maximum Pressure: less than 4.5psi (0.3 bar)

Storage:

4 °C - 8 °C in 20% ethanol

Shelf Life:

3 years

Description:

LifeTein Protein G Agarose consists of recombinant Protein G that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose. Protein G is especially suited for use with mouse antibodies in addition to most IgG isotypes from human, goat, cow and sheep sera, including human IgG3 and mouse IgG1 isotypes.

Protein G is a bacterial cell wall protein original from group G Streptococcus and now produced as a recombinant in E. coli. Recombinant Protein G has a mass of approximately 31 to 34 kDa by SDS-PAGE. IgG-binding function is optimal at pH 5 but also occurs efficiently in near-neutral conditions (pH 7.0 to 7.2). Protein G binds to most mammalian immunoglobulins primarily through their Fc regions.

Compared to Protein A, Protein G binds a broader spectrum of IgG subclasses from human, mouse and rat serum. Protein G exhibits stronger binding to human IgG3, mouse IgG1 and all three isotypes of Rat IgG. However Protein G binds more weakly than Protein A to pig, guinea pig, dog and cat IgG.

Protocol:

Protein G Binding buffer: 20 mM sodium phosphate, 150 mM NaCl, pH 7.4

Protein G Elution buffer: 100 mM Glycine, pH 3.0

Protein G Neutralization buffer: 1M Tris buffer, pH 7.5-9

Wash the prepacked column with 5~10 column volumes of distilled water to remove 20% ethanol.

Equilibrate the column with 5~10 column volumes of binding buffer.

1:10 dilution of serum with binding buffer. Filtrate the diluted serum through a 0.45 μm filter and load the sample.

Wash with 10 column volumes of binding buffer.

Elute with 5 column volumes of elution buffer and neutralize collect fractions with neutralization buffer.

After each separation cycle, regenerate the resin by washing with approximately 3~5 column volumes of 0.1 M citrate buffer (pH 3.0).

Confirm the purity of the collected antibody by SDS-PAGE analysis.

Purification capacity: ≥30 mg of rabbit IgG per ml of recombinant protein G agarose gel.

Notes:

Avoid air bubbles.

Regenerate the resin every 5 purification in order to maintain the product efficiency.

After every 10 separation cycle, wash the resin with 5 column volumes of 20% ethanol and 5~10 column volumes of 70% ethanol sequentially to remove hydrophobic substances.

Keep at 4~8 °C in 20% ethanol.

For research use only!

温馨提示：不可用于临床ZL。

您可能感兴趣的产品

Catalog Number:	LT12015
Packing Details:	2 mL resin, crosslinked 6% beaded agarose supplied as 50% slurry (e.g., 2mL of settled resin is equivalent to 4mL of 50% slurry) in 0.02% sodium azide
Binding Capacity:	20-25 mg human IgG per mL of settled resin
Support pH Stability:	2-14 (short term); 3-13 (long term)
Average Particle Size:	45 to 165 microns
Exclusion Limit:	10,000 to 4,000,000 daltons
Maximum Volumetric Flow Rate:	approx. 1mL/minute (for 1cm diameter column); Maximum Linear Velocity: 30cm per hour; Maximum Pressure: less than 4.5psi (0.3 bar)
Storage:	4 °C - 8 °C in 20% ethanol
Shelf Life:	3 years
Description:	LifeTein Protein G Agarose consists of recombinant Protein G that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose. Protein G is especially suited for use with mouse antibodies in addition to most IgG isotypes from human, goat, cow and sheep sera, including human IgG3 and mouse IgG1 isotypes. Protein G is a bacterial cell wall protein original from group G Streptococcus and now produced as a recombinant in E. coli. Recombinant Protein G has a mass of approximately 31 to 34 kDa by SDS-PAGE. IgG-binding function is optimal at pH 5 but also occurs efficiently in near-neutral conditions (pH 7.0 to 7.2). Protein G binds to most mammalian immunoglobulins primarily through their Fc regions. Compared to Protein A, Protein G binds a broader spectrum of IgG subclasses from human, mouse and rat serum. Protein G exhibits stronger binding to human IgG3, mouse IgG1 and all three isotypes of Rat IgG. However Protein G binds more weakly than Protein A to pig, guinea pig, dog and cat IgG. Protocol: Protein G Binding buffer: 20 mM sodium phosphate, 150 mM NaCl, pH 7.4 Protein G Elution buffer: 100 mM Glycine, pH 3.0 Protein G Neutralization buffer: 1M Tris buffer, pH 7.5-9 Wash the prepacked column with 5~10 column volumes of distilled water to remove 20% ethanol. Equilibrate the column with 5~10 column volumes of binding buffer. 1:10 dilution of serum with binding buffer. Filtrate the diluted serum through a 0.45 μm filter and load the sample. Wash with 10 column volumes of binding buffer. Elute with 5 column volumes of elution buffer and neutralize collect fractions with neutralization buffer. After each separation cycle, regenerate the resin by washing with approximately 3~5 column volumes of 0.1 M citrate buffer (pH 3.0). Confirm the purity of the collected antibody by SDS-PAGE analysis. Purification capacity: ≥30 mg of rabbit IgG per ml of recombinant protein G agarose gel. Notes:	Avoid air bubbles. Regenerate the resin every 5 purification in order to maintain the product efficiency. After every 10 separation cycle, wash the resin with 5 column volumes of 20% ethanol and 5~10 column volumes of 70% ethanol sequentially to remove hydrophobic substances. Keep at 4~8 °C in 20% ethanol.
	For research use only!