| Anti GAPDH Monoclonal Antibody | 说明书下载 | Calatog Number: | LT9995 | Lot Number: | 411005100211 | Isotype: | Mouse IgG1 | Clonality: | GA1R | Quantity: | 50 μg | Immunogen: | Recombinant GAPDH | Specificity: | Recognizes native and denatured forms of GAPDH (~37kDa) | Cross Reactivity: | GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster. GAPDH from other species may also be detectable | Purification: | Protein A affinity chromatography from mouse ascites fluid | Applications: | Dot, ELISA, IS, WB | Working Condition: | WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test | Storage: | Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks | Images: | Fig. LEFT: 1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). RIGHT: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3) | td> |