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Anti β-Actin Monoclonal Antibody
Calatog Number: |
LT0427 |
Lot Number: |
411005100207 |
Isotype: |
Mouse IgG2b |
Clonality: |
BA3R |
Quantity: |
50 μg |
Immunogen: |
β-Actin N-terminal peptide-KLH conjugates |
Specificity: |
Recognizes native and denatured forms of β-Actin (~42kDa) |
Cross Reactivity: |
β-Actin from human, mouse, rat, rabbit, and chicken. β-Actin from other species may also be detectable |
Purification: |
Protein A affinity chromatography from mouse ascites fluid |
Applications: |
Dot, ELISA, IS, WB |
Working Condition: |
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test |
Storage: |
Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks |
Images: |
Fig. 1:1000 (1μg/mL) Ab dilution used in WB of 20μg/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4) |
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