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产品名称:尿嘧啶DNA糖苷酶
英文名称:Uracil-DNA Glycosylase
产品货号:GS2210
产品规格:5×200U
储存条件:冷冻(-20℃)
技术规格 Unit definition | One unit is defined as the amount of enzyme that catalyzes the release of 60pmolof uracil per minute from double-stranded,uracil-containing DNA.Activity is measured by release of[3H]-uracil in a 50μlreaction containing 0,2μgDNA(104?~105?cpm/μg)in 30minutesat 37℃. |
来源 | Isolated from the recombinant source |
Optimal buffer | SE-buffer Uracil DNA Glycosylase,(20mMTris-HCl(pH 8.0 at 25℃);1 mM EDTA;1 mM DTT) |
Optimal temperature | 37℃ |
Storage conditions | 10 mM Tris-HCl(pH 7.6);50 mM NaCl;1 mM EDTA;1 mM DTT;50%glycerol.Store at -20℃. |
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