Rat NRP2 / Neuropilin 2 ELISA Kit (Sandwich ELISA) - LS-F55324Catalog Size Price LS-F55324-1 1 plate $900 USD Available Discounts 10% off 5+ units Request Bulk Add to Wish List Add to Cart SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F55324 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat NRP2 / Neuropilin 2 in samples of Cell Culture Supernatants, Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of NRP2 / Neuropilin 2 as low as 29.2 picograms per milliliter.SpecificationsType
This assay has high sensitivity and excellent specificity for detection of Neuropilin 2 (NRP2). No significant cross-reactivity or interference between Neuropilin 2 (NRP2) and analogues was observed. Manual
Intended Sample Types
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates Format
96-Well Strip Plate Detection
Colorimetric - 450nm (TMB) Measurement
Quantitative Detection Range
62.5 - 4000 pg/ml Sensitivity
29.2 pg/ml Assay Time
3 h Precision
Intra-Assay: CV<10% / Inter-Assay: CV<12% Storage
Short term: 4°C; Long term: see manual. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers
Protocol
Prepare all reagents, samples and standards.
Add 100 uL standard or sample to each well.
Incubate 2 hours at 37ºC.
Aspirate and add 100 uL prepared Detection Reagent A.
Incubate 1 hour at 37ºC.
Aspirate and wash 3 times.
Add 100 uL prepared Detection Reagent B.
Incubate 30 minutes at 37ºC.
Aspirate and wash 5 times.
Add 90 uL Substrate Solution.
Incubate 10-20 minutes at 37ºC.
Add 50 uL Stop Solution.
Read at 450nm immediately.
Restrictions
For research use only. Guarantee
This elisa kit carries the LSBio Guarantee. About NRP2 / Neuropilin 2
O60462 NM_201266 NP_957718.1
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Rat NRP2 / Neuropilin 2 ELISA Kit (Sandwich ELISA) - LS-F55324
