应用 | 分离DNA和RNA期间使核苷酸酶蛋白失活。 去除内毒素对阳离子蛋白的约束,如溶菌酶和核糖核酸酶。 研究报告显示用于分离肝,酵母,绿豆线粒体 测定细胞膜上的酶 用于抗体标记,石蜡包埋组织切片中暴露抗原结合位点。 消化脑组织样本中的蛋白质,用于朊病毒的传染性海绵状脑病(TSE)的研究 |
产品介绍 | 蛋白酶K是一个稳定,高活性丝氨酸蛋白酶。从晶体和分子结构研究的证据表明这种酶属于枯草杆菌家族,带有活性催化位点催化三键(Asp39,His69 - Ser224)。它是在宽的的环境范围内保持稳定:pH值,缓冲盐,去垢剂(SDS),和温度。在0.1-0.5% SDS存在下,在分离DNA中,蛋白酶K保持活性,消化各种蛋白质和核酸酶。 |
备注 | Unit Definition One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37℃(color by Folin-Ciocalteu reagent). |
生化机理 | Proteinase K is a stable and highly reactive serine protease.Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad(Asp39-His69-Ser224).It is stable in a broad range of environments:pH,buffer salts,detergents(SDS),and temperature.In the presence of 0.1-0.5% SDS,proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. |
别名 | 蛋白酶K;Proteinase K;Endopeptidase KProkProtease KTritirachium album proteinase K |