Mouse MBL2 / Mannose Binding Protein ELISA Kit (Sandwich ELISA) - LS-F10491

Mouse MBL2 / Mannose Binding Protein ELISA Kit (Sandwich ELISA) - LS-F10491Catalog
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LS-F10491-1
1 plate
$665 USD
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SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F10491 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse MBL2 / Mannose Binding Protein in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of MBL2 / Mannose Binding Protein as low as 10 picograms per milliliter.

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SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
MBL2 / Mannose Binding Protein
Reactivity
 
Mouse
Specificity
 
This kit is for the detection of natural and recombinant mouse MBL2. No significant cross-reactivity or interference between MBL2 and analogs was observed.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Plasma, Serum
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
312 - 20000 pg/ml
Sensitivity
 
10 pg/ml
Precision
 
Intra-Assay: CV%<4.9% Inter-Assay: CV%<6.4%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
About MBL2 / Mannose Binding Protein
 
Uniprot: P11226 NCBI: NM_000242 NP_000233.1

 

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