Human MBL2 / Mannose Binding Protein ELISA Kit (Sandwich ELISA) - LS-F10096

Human MBL2 / Mannose Binding Protein ELISA Kit (Sandwich ELISA) - LS-F10096Catalog
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LS-F10096-1
1 plate
$800 USD
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SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F10096 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human MBL2 / Mannose Binding Protein in samples of Serum. It is based upon a Sandwich assay principle and can be used to detect levels of MBL2 / Mannose Binding Protein as low as 0.39 nanograms per millilter.

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SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
MBL2 / Mannose Binding Protein
Reactivity
 
Human
Specificity
 
This assay has high sensitivity and excellent specificity for detection of human MBP/MBL. No significant cross-reactivity or interference between human MBP/MBL and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human MBP/MBL and all the analogs, therefore, cross reaction may still exist.
Manual
 

Intended Sample Types
 
Serum
Sample Size
 
50 - 100 µl
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
1.563 - 100 ng/ml
Sensitivity
 
0.39 ng/ml
Precision
 
Intra-assay: CV%<8% Inter-assay: CV%<10%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MBP/MBL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBP/MBL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBP/MBL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBP/MBL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
About MBL2 / Mannose Binding Protein
 
Uniprot: P11226 NCBI: NM_000242 NP_000233.1

 

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