CD303 (BDCA-2)-Biotin/CD303 (BDCA-2) antibodies, human

品Pai:miltenyi，产品名称：CD303 (BDCA-2)-Biotin, human
CD303 (BDCA-2) antibodies, human - Miltenyi Biotec
货号：130-090-691
CD303 (BDCA-2) antibodies have been used, for example, to identify, characterize, and enumerate plasmacytoid dendritic cells in whole blood of healthy and HIV-infected individuals, and for analyzing the role of DC-SIGN in HIV infection and transmission.^7,11 Furthermore, CD303 (BDCA-2) antibodies were used to identify and enumerate plasmacytoid dendritic cells in blood and bone marrow samples before and after hematopoietic stem cell mobilization⁸ or transplantation¹⁰. CD303 (BDCA-2) antibodies were also used for immunohistochemical staining, for example to identify plasmacytoid dendritic cells in tissue sections from patients with different inflammatory skin diseases.^5,12,13
Clone AC144 recognizes the the CD303 (BDCA-2) antigen^1,3 which is expressed on human plasmacytoid dendritic cells in blood, lymphoid (e.g. tonsils and bone marrow) and non-lymphoid tissue.^3,6 Specific expression allows direct identification of plasmacytoid dendritic cells using just one marker.^1–11 CD303 (BDCA-2)⁺ plasmacytoid dendritic cells in blood and bone marrow are CD11c^–, CD123^high, CD4⁺, Lin^–, CD45RA⁺, CD304 (BDCA-4/Neuropilin-1)⁺, CD141 (BDCA-3)^low, CD1c (BDCA-1)^–, CD14^–, and CD2^–. They express neither myeloid lineage markers (CD13, CD33) nor Fc receptors (CD32, CD64, FcRI).^1,6 CD303 (BDCA-2) is strongly expressed on freshly isolated plasmacytoid dendritic cells but down-regulated within 48 hours of culturing.¹ Unlike CD304 (BDCA-4/Neuropilin-1), CD303 (BDCA-2) is not detectable on ex vivo generated monocyte-derived or hematopoietic precursor cell-derived CD1a⁺ dendritic cells.^1,9
The CD303 (BDCA-2) antigen is a novel type II transmembrane C-type lectin.³ Remarkably, plasmacytoid dendritic cells can take up ligands via CD303 (BDCA-2), then process and present the ligands to T cells.^1,3 Unlike binding of antibodies to CD304 (BDCA-4), binding of antibodies to CD303 (BDCA-2) inhibits type I IFN production, which is induced in plasmacytoid dendritic cells by, for example, the influenza virus.^3,6

Alternative namesCLEC4C, BDCA-2, CLECSF11, CLECSF7, DLEC, HECL, PRO34150

Clone and isotype

Clone	Isotype
AC144	mouse IgG1κ