Stat Antibody Sampler Kit

品Pai:CST，产品名称：Stat Antibody Sampler Kit #9939
英文名称：Stat Antibody Sampler Kit
Product name：Stat Antibody Sampler Kit #9939
Purification： Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Stat1. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu60 of human Stat5, Gly582 of human Stat6, and a Stat3 fusion protein corresponding to the carboxy-terminal sequence of mouse Stat3.
Background： Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, -2, -3, -4 and -5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and CNTF (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses and stem cell differentiation (6-11).

Specificity / Sensitivity:

Each Stat antibody in the kit recognizes only its target protein, independent of phosphorylation state.

Protocols

• 4904:Western Blotting, Immunoprecipitation (Magnetic), ChIP Magnetic
• 5397:Western Blotting, Immunoprecipitation (Magnetic), ChIP Magnetic
• 7074:Western Blotting
• 9172:Western Blotting, Immunoprecipitation (Magnetic), ChIP Magnetic
• 94205:Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic

Product Description:

The Stat Antibody Sampler Kit provides an economical means to examine multiple Stat proteins: Stat1, Stat3, Stat5 and Stat6. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.