Anti-AASDHPPT antibody_详细资料

产品信息

货号： JLC221436 保存条件： -20°C 浓度： 0.3 mg/ml 宿主： Rabbit 抗体英文名： Anti-AASDHPPT antibody 保质期： 1年克隆性：多克隆数量：大量供应商：江西江蓝纯生物试剂有限公司规格： 25 μl/100 μl/200 μl

Anti-AASDHPPT antibody

Cat. No.    JLC221436
Package   25 μl/100 μl/200 μl
Storage   -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description    Anti-AASDHPPT rabbit polyclonal antibody
Applications     ELISA, WB, IHC
Immunogen    Fusion protein of human AASDHPPT
Reactivity    Human, Mouse, Rat
Content    0.3 mg/ml
Host species    Rabbit
Ig class     Immunogen-specific rabbit IgG
Purification    Antigen affinity purification

Target information
Symbol    AASDHPPT
Full name    Aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
Synonyms   LYS2; LYS5; CGI-80; AASD-PPT
Swissprot   Q9NRN7

Target Background
The protein encoded by this gene is similar to Saccharomyces cerevisiae LYS5, which is required for the activation of the alpha-aminoadipate dehydrogenase in the biosynthetic pathway of lysine. Yeast alpha-aminoadipate dehydrogenase converts alpha-biosynthetic-aminoadipate semialdehyde to alpha-aminoadipate. It has been suggested that defects in the human gene result in pipecolic acidemia.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 50-200   Predicted cell location: Cytoplasm
Positive control: Human brain
Recommended dilution: 50-200

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml221436(AASDHPPT Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)   The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml221436(AASDHPPT Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:36 kDa
Positive control:Human fetal brain tissue
Recommended dilution: 500-2000

Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane: Human fetal brain tissue
Primary antibody: ml221436(AASDHPPT Antibody) at dilution 1/650
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 30 seconds

ELISA
Recommended dilution: 2000-5000

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