Rat EPHX1 / Epoxide Hydrolase 1 ELISA Kit (Sandwich ELISA) - LS-F53766

Rat EPHX1 / Epoxide Hydrolase 1 ELISA Kit (Sandwich ELISA) - LS-F53766
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Size
Price
LS-F53766-1
1 plate
$1,090 USD
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10% off 5+ units
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SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F53766 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat EPHX1 / Epoxide Hydrolase 1 in samples of Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of EPHX1 / Epoxide Hydrolase 1 as low as 3.9 picograms per milliliter.
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SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
EPHX1 / Epoxide Hydrolase 1
Synonyms
 
EPHX1, Epoxide hydratase, Epoxide hydrolase, Epoxide hydrolase 1, HYL1, MEH, Microsomal epoxide hydrolase, EPHX, EPOX
Reactivity
 
Rat
Specificity
 
This assay has high sensitivity and excellent specificity for detection of rat EPHX1. No significant cross-reactivity or interference between rat EPHX1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between rat EPHX1 and all the analogues, therefore, cross reaction may still exist.
Manual
 

Intended Sample Types
 
Cell Lysates, Plasma, Serum, Tissue Homogenates
Sample Size
 
50 - 100 µl
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
15.6 - 1000 pg/ml
Sensitivity
 
3.9 pg/ml
Assay Time
 
4.5 h
Precision
 
Intra-Assay: CV<8% / Inter-Assay: CV<10%
Storage
 
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
About EPHX1 / Epoxide Hydrolase 1
 
Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene. Uniprot: P07099 NCBI: NM_000120 NP_000111.1

 

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Sandwich ELISA Platform Overview