Human AOAH / Acyloxyacyl Hydrolase ELISA Kit (Sandwich ELISA) - LS-F8116

Human AOAH / Acyloxyacyl Hydrolase ELISA Kit (Sandwich ELISA) - LS-F8116

Catalog Number: LS-F8116 Type: Sandwich ELISA (enzyme-linked immunosorbent assay) kit Target: AOAH / Acyloxyacyl Hydrolase Reactivity: Human Specificity: Acyloxyacyl Hydrolase (AOAH) No significant cross-reactivity or interference between Acyloxyacyl Hydrolase (AOAH) and analogs was observed. Description: LS-F8116 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human AOAH / Acyloxyacyl Hydrolase in samples of Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of AOAH / Acyloxyacyl Hydrolase as low as 0.058 nanograms per millilter.

Intended Sample Types: Cell Lysates, Plasma, Serum, Tissue Homogenates

Format: 96-Well Strip Plate Detection: Colorimetric - 450nm (TMB) Detection Range: 0.156 - 10 ng/ml Sensitivity: 0.058 ng/ml Assay Time: 4.5 h Precision: Intra-Assay: CV<10% Inter-Assay: CV<12%

Quality Assurance: Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Kit Components: Coated 96-well Strip Plate Adhesive Plate Sealers Standard (Lyophilized) Sample Diluent Detection Reagent A (Green) Detection Reagent B (Red) Assay Diluent A Assay Diluent B TMB Substrate Stop Solution Wash Buffer (30x concentrate)

Storage: Short term: 4°C; Long term: see manual. Restrictions: For research use only.

About AOAH / Acyloxyacyl HydrolaseUniprot: P28039 NCBI: NM_001637 NP_001628.1

Synonyms: AOAH ElisaKit, Acyloxyacyl hydrolase ElisaKit

Description: This locus encodes both the light and heavy subunits of acyloxyacyl hydrolase. The encoded enzyme catalyzes the hydrolysis of acyloxylacyl-linked fatty acyl chains from bacterial lipopolysaccharides, effectively detoxifying these molecules. The encoded protein may play a role in modulating host inflammatory response to gram-negative bacteria. Alternatively spliced transcript variants have been described.
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