性质
| Related Categories | 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Coagulation Proteins and Reagents, Core Bioreagents,
更多... |
| form | lyophilized powder |
| impurities | HIV and HBsAg, source material tested negative |
| storage temp. | −20°C |
| Gene Information | human ... F2(2147) |
产品描述
Analysis Note
NIH 试验步骤基于 Biggs 方法的改进，使用 0.2mL 稀释血浆（与生理盐水 1:1 混合，作为底物）和 0.1mL Biggs 凝血酶样品（稳定于 1% 缓冲白蛋白溶液中）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。
Biochem/physiol Actions
丝氨酸蛋白酶，可选择性裂解血纤维蛋白原的 Arg-Gly 键，形成血纤维蛋白和血纤维蛋白肽 A 和 B。
General description
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
Other Notes
查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。
Unit Definition
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
Physical form
Lyophilized from saline sodium citrate buffer, pH 6.5
Application
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to investigate the protein C pathway in intestinal barrier function. [1]
温馨提示：不可用于临床ZL。
