更多质粒信息请到官方网站
标题1测序及更多信息：https://pan.baidu.com/s/1QFW27WjhG3L3BX5mntfjPQ 密码：ntdm采购地址：淘宝店铺地址
| 载体名称: | pPSUMO |
| 质粒类型: | 大肠杆菌基因表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | T7 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5633 bp |
| 5' 测序引物及序列: | T7: 5'-TAATACGACTCACTATAGGG-3' |
| 3' 测序引物及序列: | T7t: 5'-GCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-His; N-SUMO |
| 载体抗性: | Kanamycin 卡那霉素 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
载体组建方法
This expression vector (termed ppSUMO) was a gift fromP. Pellicini and was originally derived from pET28(Novagen), with the open reading frame for yeast smt3 inserted between the NcoI and BamHI sites. The open reading frame for full-length AAG was PCR-amplified, digested with BamHI and XhoI, and ligated into the ppSUMO vector that had been cut by the same enzymes. The desired construct was verified by sequencing the entire open reading frame of the SUMO fusion. This new expression construct greatly increased the yields of the full-length protein.
Baldwin M R, O’Brien P J. Nonspecific DNA binding and coordination of the first two steps of base excision repair[J]. Biochemistry, 2010, 49(36): 7879-7891.
全序列
1 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC
61 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA121 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA181 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA241 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA301 AAATATTAAC GTTTACAATT TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA361 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAATTAA TTCTTAGAAA421 AACTCATCGA GCATCAAATG AAACTGCAAT TTATTCATAT CAGGATTATC AATACCATAT481 TTTTGAAAAA GCCGTTTCTG TAATGAAGGA GAAAACTCAC CGAGGCAGTT CCATAGGATG541 GCAAGATCCT GGTATCGGTC TGCGATTCCG ACTCGTCCAA CATCAATACA ACCTATTAAT601 TTCCCCTCGT CAAAAATAAG GTTATCAAGT GAGAAATCAC CATGAGTGAC GACTGAATCC661 GGTGAGAATG GCAAAAGTTT ATGCATTTCT TTCCAGACTT GTTCAACAGG CCAGCCATTA721 CGCTCGTCAT CAAAATCACT CGCATCAACC AAACCGTTAT TCATTCGTGA TTGCGCCTGA781 GCGAGACGAA ATACGCGATC GCTGTTAAAA GGACAATTAC AAACAGGAAT CGAATGCAAC841 CGGCGCAGGA ACACTGCCAG CGCATCAACA ATATTTTCAC CTGAATCAGG ATATTCTTCT901 AATACCTGGA ATGCTGTTTT CCCGGGGATC GCAGTGGTGA GTAACCATGC ATCATCAGGA961 GTACGGATAA AATGCTTGAT GGTCGGAAGA GGCATAAATT CCGTCAGCCA GTTTAGTCTG1021 ACCATCTCAT CTGTAACATC ATTGGCAACG CTACCTTTGC CATGTTTCAG AAACAACTCT1081 GGCGCATCGG GCTTCCCATA CAATCGATAG ATTGTCGCAC CTGATTGCCC GACATTATCG1141 CGAGCCCATT TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCCTAGAG1201 CAAGACGTTT CCCGTTGAAT ATGGCTCATA ACACCCCTTG TATTACTGTT TATGTAAGCA1261 GACAGTTTTA TTGTTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC1321 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG1381 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT1441 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT1501 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT1561 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG1621 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC1681 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA1741 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG1801 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA1861 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG1921 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG1981 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT2041 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC2101 AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG2161 TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA2221 AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC2281 CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG2341 CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG2401 CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT2461 GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA2521 AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG2581 GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG2641 TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT2701 GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG2761 ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG2821 TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC2881 ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA2941 TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG3001 ACAGGAGCAC GATCATGCGC ACCCGTGGGG CCGCCATGCC GGCGATAATG GCCTGCTTCT3061 CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC3121 CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA3181 AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA3241 TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG3301 CTCTCAAGGG CATCGGTCGA GATCCCGGTG CCTAATGAGT GAGCTAACTT ACATTAATTG3361 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA3421 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CCAGGGTGGT TTTTCTTTTC3481 ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT GGCCCTGAGA GAGTTGCAGC3541 AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT GTTTGATGGT GGTTAACGGC3601 GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA CTACCGAGAT ATCCGCACCA3661 ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA GCGCCATCTG ATCGTTGGCA3721 ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT GCATGGTTTG TTGAAAACCG3781 GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT GAATTTGATT GCGAGTGAGA3841 TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG AACTTAATGG GCCCGCTAAC3901 AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA CGCCCAGTCG CGTACCGTCT3961 TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG AGACATCAAG AAATAACGCC4021 GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT GGTCATCCAG CGGATAGTTA4081 ATGATCAGCC CACTGACGCG TTGCGCGAGA AGATTGTGCA CCGCCGCTTT ACAGGCTTCG4141 ACGCCGCTTC GTTCTACCAT CGACACCACC ACGCTGGCAC CCAGTTGATC GGCGCGAGAT4201 TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGGCCA GACTGGAGGT GGCAACGCCA4261 ATCAGCAACG ACTGTTTGCC CGCCAGTTGT TGTGCCACGC GGTTGGGAAT GTAATTCAGC4321 TCCGCCATCG CCGCTTCCAC TTTTTCCCGC GTTTTCGCAG AAACGTGGCT GGCCTGGTTC4381 ACCACGCGGG AAACGGTCTG ATAAGAGACA CCGGCATACT CTGCGACATC GTATAACGTT4441 ACTGGTTTCA CATTCACCAC CCTGAATTGA CTCTCTTCCG GGCGCTATCA TGCCATACCG4501 CGAAAGGTTT TGCGCCATTC GATGGTGTCC GGGATCTCGA CGCTCTCCCT TATGCGACTC4561 CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA4621 TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC4681 CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT4741 GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG4801 TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAATTAA TACGACTCAC TATAGGGGAA4861 TTGTGAGCGG ATAACAATTC CCCTCTAGAA ATAATTTTGT TTAACTTTAA GAAGGAGATA4921 TACCATGGGC AGCAGCCATC ATCATCATCA TCACAGCAGC GGCCTGGTGC CGCGCGGCAG4981 CCATATGGCT AGCATGTCGG ACTCAGAAGT CAATCAAGAA GCTAAGCCAG AGGTCAAGCC5041 AGAAGTCAAG CCTGAGACTC ACATCAATTT AAAGGTGTCC GATGGATCTT CAGAGATCTT5101 CTTCAAGATC AAAAAGACCA CTCCTTTAAG AAGGCTGATG GAAGCGTTCG CTAAAAGACA5161 GGGTAAGGAA ATGGACTCCT TAAGATTCTT GTACGACGGT ATTAGAATCC AAGCTGATCA5221 GACCCCTGAA GATTTGGACA TGGAGGATAA CGATATTATT GAGGCTCACA GAGAACAGAT5281 TGGTGGATCC GAATTCGAGC TCCGTCGACA AGCTTGCGGC CGCACTCGAG CACCACCACC5341 ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA5401 CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTC
温馨提示：不可用于临床ZL。
pPSUMO 大肠杆菌基因表达 SUMO pET-28-SUMO
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优势批发 大肠杆菌宿主残留蛋白（E.coli P）ELISA Kit
大肠杆菌宿主残留蛋白（E.coli P）ELISA Kit
大肠杆菌测试片
餐具大肠杆菌测试片
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