eSpCas9(1.1) 基因敲除载体；CRISPR-Cas9；plasmid #71814_详细资料

产品信息

货号： HH-CAS9-046 供应商：上海海吉浩格生物科技有限公司数量： 100 英文名： HedgehogBio 保存条件： -20 规格：质粒菌液

更多质粒信息请到官方网站

测序及更多信息： https://pan.baidu.com/s/1MBR9TXOAJPNrgFT-QJ5w6A 密码：s3o7

采购地址：淘宝店铺地址
载体基本信息

载体名称:

eSpCas9(1.1)

质粒类型:

CRISPR-Cas9载体；基因敲除载体；哺乳动物载体

高拷贝/低拷贝:

高拷贝

插入位点:

限制性内切酶，多克隆位点

启动子:

Cbh

载体大小:

8506 bp

5' 测序引物及序列:

AGGGATGGTTGGTTGGTGGG

3' 测序引物及序列:

CCAATCCTCCCCCTTGCTGT

载体标签:

Flag、NLS (N ter)

载体抗性:

氨苄青霉素

筛选标记:

--

克隆菌株:

Stbl3

宿主细胞（系）:

哺乳动物细胞

稳定性:

瞬表达

组成型/诱导型:

组成型

病毒/非病毒:

非病毒

全序列
1 GGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG GGGCGGGGCG
61 AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT TCCTTTTATG
121 GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC GGGAGTCGCT
181 GCGCGCTGCC TTCGCCCCGT GCCCCGCTCC GCCGCCGCCT CGCGCCGCCC GCCCCGGCTC
241 TGACTGACCG CGTTACTCCC ACAGGTGAGC GGGCGGGACG GCCCTTCTCC TCCGGGCTGT
301 AATTAGCTGA GCAAGAGGTA AGGGTTTAAG GGATGGTTGG TTGGTGGGGT ATTAATGTTT
361 AATTACCTGG AGCACCTGCC TGAAATCACT TTTTTTCAGG TTGGACCGGT GCCACCATGG
421 ACTATAAGGA CCACGACGGA GACTACAAGG ATCATGATAT TGATTACAAA GACGATGACG
481 ATAAGATGGC CCCAAAGAAG AAGCGGAAGG TCGGTATCCA CGGAGTCCCA GCAGCCGACA
541 AGAAGTACAG CATCGGCCTG GACATCGGCA CCAACTCTGT GGGCTGGGCC GTGATCACCG
601 ACGAGTACAA GGTGCCCAGC AAGAAATTCA AGGTGCTGGG CAACACCGAC CGGCACAGCA
661 TCAAGAAGAA CCTGATCGGA GCCCTGCTGT TCGACAGCGG CGAAACAGCC GAGGCCACCC
721 GGCTGAAGAG AACCGCCAGA AGAAGATACA CCAGACGGAA GAACCGGATC TGCTATCTGC
781 AAGAGATCTT CAGCAACGAG ATGGCCAAGG TGGACGACAG CTTCTTCCAC AGACTGGAAG
841 AGTCCTTCCT GGTGGAAGAG GATAAGAAGC ACGAGCGGCA CCCCATCTTC GGCAACATCG
901 TGGACGAGGT GGCCTACCAC GAGAAGTACC CCACCATCTA CCACCTGAGA AAGAAACTGG
961 TGGACAGCAC CGACAAGGCC GACCTGCGGC TGATCTATCT GGCCCTGGCC CACATGATCA
1021 AGTTCCGGGG CCACTTCCTG ATCGAGGGCG ACCTGAACCC CGACAACAGC GACGTGGACA
1081 AGCTGTTCAT CCAGCTGGTG CAGACCTACA ACCAGCTGTT CGAGGAAAAC CCCATCAACG
1141 CCAGCGGCGT GGACGCCAAG GCCATCCTGT CTGCCAGACT GAGCAAGAGC AGACGGCTGG
1201 AAAATCTGAT CGCCCAGCTG CCCGGCGAGA AGAAGAATGG CCTGTTCGGA AACCTGATTG
1261 CCCTGAGCCT GGGCCTGACC CCCAACTTCA AGAGCAACTT CGACCTGGCC GAGGATGCCA
1321 AACTGCAGCT GAGCAAGGAC ACCTACGACG ACGACCTGGA CAACCTGCTG GCCCAGATCG
1381 GCGACCAGTA CGCCGACCTG TTTCTGGCCG CCAAGAACCT GTCCGACGCC ATCCTGCTGA
1441 GCGACATCCT GAGAGTGAAC ACCGAGATCA CCAAGGCCCC CCTGAGCGCC TCTATGATCA
1501 AGAGATACGA CGAGCACCAC CAGGACCTGA CCCTGCTGAA AGCTCTCGTG CGGCAGCAGC
1561 TGCCTGAGAA GTACAAAGAG ATTTTCTTCG ACCAGAGCAA GAACGGCTAC GCCGGCTACA
1621 TTGACGGCGG AGCCAGCCAG GAAGAGTTCT ACAAGTTCAT CAAGCCCATC CTGGAAAAGA
1681 TGGACGGCAC CGAGGAACTG CTCGTGAAGC TGAACAGAGA GGACCTGCTG CGGAAGCAGC
1741 GGACCTTCGA CAACGGCAGC ATCCCCCACC AGATCCACCT GGGAGAGCTG CACGCCATTC
1801 TGCGGCGGCA GGAAGATTTT TACCCATTCC TGAAGGACAA CCGGGAAAAG ATCGAGAAGA
1861 TCCTGACCTT CCGCATCCCC TACTACGTGG GCCCTCTGGC CAGGGGAAAC AGCAGATTCG
1921 CCTGGATGAC CAGAAAGAGC GAGGAAACCA TCACCCCCTG GAACTTCGAG GAAGTGGTGG
1981 ACAAGGGCGC TTCCGCCCAG AGCTTCATCG AGCGGATGAC CAACTTCGAT AAGAACCTGC
2041 CCAACGAGAA GGTGCTGCCC AAGCACAGCC TGCTGTACGA GTACTTCACC GTGTATAACG
2101 AGCTGACCAA AGTGAAATAC GTGACCGAGG GAATGAGAAA GCCCGCCTTC CTGAGCGGCG
2161 AGCAGAAAAA GGCCATCGTG GACCTGCTGT TCAAGACCAA CCGGAAAGTG ACCGTGAAGC
2221 AGCTGAAAGA GGACTACTTC AAGAAAATCG AGTGCTTCGA CTCCGTGGAA ATCTCCGGCG
2281 TGGAAGATCG GTTCAACGCC TCCCTGGGCA CATACCACGA TCTGCTGAAA ATTATCAAGG
2341 ACAAGGACTT CCTGGACAAT GAGGAAAACG AGGACATTCT GGAAGATATC GTGCTGACCC
2401 TGACACTGTT TGAGGACAGA GAGATGATCG AGGAACGGCT GAAAACCTAT GCCCACCTGT
2461 TCGACGACAA AGTGATGAAG CAGCTGAAGC GGCGGAGATA CACCGGCTGG GGCAGGCTGA
2521 GCCGGAAGCT GATCAACGGC ATCCGGGACA AGCAGTCCGG CAAGACAATC CTGGATTTCC
2581 TGAAGTCCGA CGGCTTCGCC AACAGAAACT TCATGCAGCT GATCCACGAC GACAGCCTGA
2641 CCTTTAAAGA GGACATCCAG AAAGCCCAGG TGTCCGGCCA GGGCGATAGC CTGCACGAGC
2701 ACATTGCCAA TCTGGCCGGC AGCCCCGCCA TTAAGAAGGG CATCCTGCAG ACAGTGAAGG
2761 TGGTGGACGA GCTCGTGAAA GTGATGGGCC GGCACAAGCC CGAGAACATC GTGATCGAAA
2821 TGGCCAGAGA GAACCAGACC ACCCAGAAGG GACAGAAGAA CAGCCGCGAG AGAATGAAGC
2881 GGATCGAAGA GGGCATCAAA GAGCTGGGCA GCCAGATCCT GAAAGAACAC CCCGTGGAAA
2941 ACACCCAGCT GCAGAACGAG AAGCTGTACC TGTACTACCT GCAGAATGGG CGGGATATGT
3001 ACGTGGACCA GGAACTGGAC ATCAACCGGC TGTCCGACTA CGATGTGGAC CATATCGTGC
3061 CTCAGAGCTT TCTGGCCGAC GACTCCATCG ACAACAAGGT GCTGACCAGA AGCGACAAGA
3121 ACCGGGGCAA GAGCGACAAC GTGCCCTCCG AAGAGGTCGT GAAGAAGATG AAGAACTACT
3181 GGCGGCAGCT GCTGAACGCC AAGCTGATTA CCCAGAGAAA GTTCGACAAT CTGACCAAGG
3241 CCGAGAGAGG CGGCCTGAGC GAACTGGATA AGGCCGGCTT CATCAAGAGA CAGCTGGTGG
3301 AAACCCGGCA GATCACAAAG CACGTGGCAC AGATCCTGGA CTCCCGGATG AACACTAAGT
3361 ACGACGAGAA TGACAAGCTG ATCCGGGAAG TGAAAGTGAT CACCCTGAAG TCCAAGCTGG
3421 TGTCCGATTT CCGGAAGGAT TTCCAGTTTT ACAAAGTGCG CGAGATCAAC AACTACCACC
3481 ACGCCCACGA CGCCTACCTG AACGCCGTCG TGGGAACCGC CCTGATCAAA AAGTACCCTG
3541 CGCTGGAAAG CGAGTTCGTG TACGGCGACT ACAAGGTGTA CGACGTGCGG AAGATGATCG
3601 CCAAGAGCGA GCAGGAAATC GGCAAGGCTA CCGCCAAGTA CTTCTTCTAC AGCAACATCA
3661 TGAACTTTTT CAAGACCGAG ATTACCCTGG CCAACGGCGA GATCCGGAAG GCGCCTCTGA
3721 TCGAGACAAA CGGCGAAACC GGGGAGATCG TGTGGGATAA GGGCCGGGAT TTTGCCACCG
3781 TGCGGAAAGT GCTGAGCATG CCCCAAGTGA ATATCGTGAA AAAGACCGAG GTGCAGACAG
3841 GCGGCTTCAG CAAAGAGTCT ATCCTGCCCA AGAGGAACAG CGATAAGCTG ATCGCCAGAA
3901 AGAAGGACTG GGACCCTAAG AAGTACGGCG GCTTCGACAG CCCCACCGTG GCCTATTCTG
3961 TGCTGGTGGT GGCCAAAGTG GAAAAGGGCA AGTCCAAGAA ACTGAAGAGT GTGAAAGAGC
4021 TGCTGGGGAT CACCATCATG GAAAGAAGCA GCTTCGAGAA GAATCCCATC GACTTTCTGG
4081 AAGCCAAGGG CTACAAAGAA GTGAAAAAGG ACCTGATCAT CAAGCTGCCT AAGTACTCCC
4141 TGTTCGAGCT GGAAAACGGC CGGAAGAGAA TGCTGGCCTC TGCCGGCGAA CTGCAGAAGG
4201 GAAACGAACT GGCCCTGCCC TCCAAATATG TGAACTTCCT GTACCTGGCC AGCCACTATG
4261 AGAAGCTGAA GGGCTCCCCC GAGGATAATG AGCAGAAACA GCTGTTTGTG GAACAGCACA
4321 AGCACTACCT GGACGAGATC ATCGAGCAGA TCAGCGAGTT CTCCAAGAGA GTGATCCTGG
4381 CCGACGCTAA TCTGGACAAA GTGCTGTCCG CCTACAACAA GCACCGGGAT AAGCCCATCA
4441 GAGAGCAGGC CGAGAATATC ATCCACCTGT TTACCCTGAC CAATCTGGGA GCCCCTGCCG
4501 CCTTCAAGTA CTTTGACACC ACCATCGACC GGAAGAGGTA CACCAGCACC AAAGAGGTGC
4561 TGGACGCCAC CCTGATCCAC CAGAGCATCA CCGGCCTGTA CGAGACACGG ATCGACCTGT
4621 CTCAGCTGGG AGGCGACAAA AGGCCGGCGG CCACGAAAAA GGCCGGCCAG GCAAAAAAGA
4681 AAAAGTAAGA ATTCCTAGAG CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC
4741 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT
4801 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT
4861 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGAGAATA GCAGGCATGC
4921 TGGGGAGCGG CCGCAGGAAC CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG
4981 CTCGCTCACT GAGGCCGGGC GACCAAAGGT CGCCCGACGC CCGGGCTTTG CCCGGGCGGC
5041 CTCAGTGAGC GAGCGAGCGC GCAGCTGCCT GCAGGGGCGC CTGATGCGGT ATTTTCTCCT
5101 TACGCATCTG TGCGGTATTT CACACCGCAT ACGTCAAAGC AACCATAGTA CGCGCCCTGT
5161 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC
5221 AGCGCCTTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC
5281 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG
5341 CACCTCGACC CCAAAAAACT TGATTTGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA
5401 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC
5461 CAAACTGGAA CAACACTCAA CTCTATCTCG GGCTATTCTT TTGATTTATA AGGGATTTTG
5521 CCGATTTCGG TCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
5581 AACAAAATAT TAACGTTTAC AATTTTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC
5641 GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT
5701 CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG
5761 AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT
5821 TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA
5881 AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC
5941 ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT
6001 CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT
6061 CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT
6121 TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT
6181 TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC
6241 GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC
6301 TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT
6361 GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG
6421 AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG
6481 GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA
6541 ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA
6601 CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT
6661 CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGAAG CCGCGGTATC
6721 ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG
6781 AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT
6841 AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT
6901 CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC
6961 CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT
7021 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA
7081 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC
7141 TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC
7201 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT
7261 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT
7321 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG
7381 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA
7441 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG
7501 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA
7561 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC
7621 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTGAGGGCCT
7681 ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTGG
7741 AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA
7801 ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC
7861 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACAC
7921 CGGGTCTTCG AGAAGACCTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC
7981 GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TGTTTTAGAG CTAGAAATAG
8041 CAAGTTAAAA TAAGGCTAGT CCGTTTTTAG CGCGTGCGCC AATTCTGCAG ACAAATGGCT
8101 CTAGAGGTAC CCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC
8161 CCCCGCCCAT TGACGTCAAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG
8221 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG
8281 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTGTGCCCA GTACATGACC
8341 TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTC
8401 GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC CCCCCTCCCC ACCCCCAATT
8461 TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG GGGCG

温馨提示：不可用于临床ZL。

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eSpCas9(1.1) 基因敲除载体；CRISPR-Cas9；plasmid #71814

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载体名称:	eSpCas9(1.1)
质粒类型:	CRISPR-Cas9载体；基因敲除载体；哺乳动物载体
高拷贝/低拷贝:	高拷贝
插入位点:	限制性内切酶，多克隆位点
启动子:	Cbh
载体大小:	8506 bp
5' 测序引物及序列:	AGGGATGGTTGGTTGGTGGG
3' 测序引物及序列:	CCAATCCTCCCCCTTGCTGT
载体标签:	Flag、NLS (N ter)
载体抗性:	氨苄青霉素
筛选标记:	--
克隆菌株:	Stbl3
宿主细胞（系）:	哺乳动物细胞
稳定性:	瞬表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒