pTRV2 植物RNAi载体 病毒诱导的基因沉默(VIGS)

测序及更多信息： 

链接：https://pan.baidu.com/s/1fmmCKXkp2zrJ5jOLo3EafA 
提取码：mru3

采购地址：淘宝店铺地址

试验方法和教程

全序列

1        ATAAAACATT GCACCTATGG TGTTGCCCTG GCTGGGGTAT GTCAGTGATC GCAGTAGAAT
61       GTACTAATTG ACAAGTTGGA GAATACGGTA GAACGTCCTT ATCCAACACA GCCTTTATCC
121      CTCTCCCTGA CGAGGTTTTT GTCAGTGTAA TATTTCTTTT TGAACTATCC AGCTTAGTAC
181      CGTACGGGAA AGTGACTGGT GTGCTTATCT TTGAAATGTT ACTTTGGGTT TCGGTTCTTT
241      AGGTTAGTAA GAAAGCACTT GTCTTCTCAT ACAAAGGAAA ACCTGAGACG TATCGCTTAC
301      GAAAGTAGCA ATGAAAGAAA GGTGGTGGTT TTAATCGCTA CCGCAAAAAC GATGGGGTCG
361      TTTTAATTAA CTTCTCCTAC GCAAGCGTCT AAACGGACGT TGGGGTTTTG CTAGTTTCTT
421      TAGAGAAAAC TAGCTAAGTC TTTAATGTTA TCATTAGAGA TGGCATAAAT ATAATACTTG
481      TGTCTGCTGA TAAGATCATT TTAATTTGGA CGATTAGACT TGTTGAACTA CAGGTTACTG
541      AATCACTTGC GCTAATCAAC ATGGGAGATA TGTACGATGA ATCATTTGAC AAGTCGGGCG
601      GTCCTGCTGA CTTGATGGAC GATTCTTGGG TGGAATCAGT TTCGTGGAAA GATCTGTTGA
661      AGAAGTTACA CAGCATAAAA TTTGCACTAC AGTCTGGTAG AGATGAGATC ACTGGGTTAC
721      TAGCGGCACT GAATAGACAG TGTCCTTATT CACCATATGA GCAGTTTCCA GATAAGAAGG
781      TGTATTTCCT TTTAGACTCA CGGGCTAACA GTGCTCTTGG TGTGATTCAG AACGCTTCAG
841      CGTTCAAGAG ACGAGCTGAT GAGAAGAATG CAGTGGCGGG TGTTACAAAT ATTCCTGCGA
901      ATCCAAACAC AACGGTTACG ACGAACCAAG GGAGTACTAC TACTACCAAG GCGAACACTG
961      GCTCGACTTT GGAAGAAGAC TTGTACACTT ATTACAAATT CGATGATGCC TCTACAGCTT
1021     TCCACAAATC TCTAACTTCG TTAGAGAACA TGGAGTTGAA GAGTTATTAC CGAAGGAACT
1081     TTGAGAAAGT ATTCGGGATT AAGTTTGGTG GAGCAGCTGC TAGTTCATCT GCACCGCCTC
1141     CAGCGAGTGG AGGTCCGATA CGTCCTAATC CCTAGGGATT TAAGGACGTG AACTCTGTTG
1201     AGATCTCTGT GAAATTCAGA GGGTGGGTGA TACCATATTC ACTGATGCCA TTAGCGACAT
1261     CTAAATAGGG CTAATTGTGA CTAATTTGAG GGAATTTCCT TTACCATTGA CGTCAGTGTC
1321     GTTGGTAGCA TTTGAGTTTC GCAATGCACG AATTACTTAG GAAGTGGCTT GACGACACTA
1381     ATGTGTTATT GTTAGATAAT GGTTTGGTGG TCAAGGTACG TAGTAGAGTC CCACATATTC
1441     GCACGTATGA AGTAATTGGA AAGTTGTCAG TTTTTGATAA TTCACTGGGA GATGATACGC
1501     TGTTTGAGGG AAAAGTAGAG AACGTATTTG TTTTTATGTT CAGGCGGTTC TTGTGTGTCA
1561     ACAAAGATGG ACATTGTTAC TCAAGGAAGC ACGATGAGCT TTATTATTAC GGACGAGTGG
1621     ACTTAGATTC TGTGAGTAAG GTTACCGAAT TCTCTAGAAG GCCTCCATGG GGATCCGGTA
1681     CCGAGCTCAC GCGTCTCGAG GCCCGGGCAT GTCCCGAAGA CATTAAACTA CGGTTCTTTA
1741     AGTAGATCCG TGTCTGAAGT TTTAGGTTCA ATTTAAACCT ACGAGATTGA CATTCTCGAC
1801     TGATCTTGAT TGATCGGTAA GTCTTTTGTA ATTTAATTTT CTTTTTGATT TTATTTTAAA
1861     TTGTTATCTG TTTCTGTGTA TAGACTGTTT GAGATCGGCG TTTGGCCGAC TCATTGTCTT
1921     ACCATAGGGG AACGGACTTT GTTTGTGTTG TTATTTTATT TGTATTTTAT TAAAATTCTC
1981     AACGATCTGA AAAAGCCTCG CGGCTAAGAG ATTGTTGGGG GGTGAGTAAG TACTTTTAAA
2041     GTGATGATGG TTACAAAGGC AAAAGGGGTA AAACCCCTCG CCTACGTAAG CGTTATTACG
2101     CCCGTCTGTA CTTATATCAG TACACTGACG AGTCCCTAAA GGACGAAACG GGAGAACGCT
2161     AGCCACCACC ACCACCACCA CGTGTGAATT ACAGGTGACC AGCTCGAATT TCCCCGATCG
2221     TTCAAACATT TGGCAATAAA GTTTCTTAAG ATTGAATCCT GTTGCCGGTC TTGCGATGAT
2281     TATCATATAA TTTCTGTTGA ATTACGTTAA GCATGTAATA ATTAACATGT AATGCATGAC
2341     GTTATTTATG AGATGGGTTT TTATGATTAG AGTCCCGCAA TTATACATTT AATACGCGAT
2401     AGAAAACAAA ATATAGCGCG CAAACTAGGA TAAATTATCG CGCGCGGTGT CATCTATGTT
2461     ACTAGATCGG GAATTAAACT ATCAGTGTTT GACAGGATAT ATTGGCGGGT AAACCTAAGA
2521     GAAAAGAGCG TTTATTAGAA TAACGGATAT TTAAAAGGGC GTGAAAAGGT TTATCCGTTC
2581     GTCCATTTGT ATGTGCATGC CAACCACAGG GTTCCCCTCG GGATCAAAGT ACTTTGATCC
2641     AACCCCTCCG CTGCTATAGT GCAGTCGGCT TCTGACGTTC AGTGCAGCCG TCTTCTGAAA
2701     ACGACATGTC GCACAAGTCC TAAGTTACGC GACAGGCTGC CGCCCTGCCC TTTTCCTGGC
2761     GTTTTCTTGT CGCGTGTTTT AGTCGCATAA AGTAGAATAC TTGCGACTAG AACCGGAGAC
2821     ATTACGCCAT GAACAAGAGC GCCGCCGCTG GCCTGCTGGG CTATGCCCGC GTCAGCACCG
2881     ACGACCAGGA CTTGACCAAC CAACGGGCCG AACTGCACGC GGCCGGCTGC ACCAAGCTGT
2941     TTTCCGAGAA GATCACCGGC ACCAGGCGCG ACCGCCCGGA GCTGGCCAGG ATGCTTGACC
3001     ACCTACGCCC TGGCGACGTT GTGACAGTGA CCAGGCTAGA CCGCCTGGCC CGCAGCACCC
3061     GCGACCTACT GGACATTGCC GAGCGCATCC AGGAGGCCGG CGCGGGCCTG CGTAGCCTGG
3121     CAGAGCCGTG GGCCGACACC ACCACGCCGG CCGGCCGCAT GGTGTTGACC GTGTTCGCCG
3181     GCATTGCCGA GTTCGAGCGT TCCCTAATCA TCGACCGCAC CCGGAGCGGG CGCGAGGCCG
3241     CCAAGGCCCG AGGCGTGAAG TTTGGCCCCC GCCCTACCCT CACCCCGGCA CAGATCGCGC
3301     ACGCCCGCGA GCTGATCGAC CAGGAAGGCC GCACCGTGAA AGAGGCGGCT GCACTGCTTG
3361     GCGTGCATCG CTCGACCCTG TACCGCGCAC TTGAGCGCAG CGAGGAAGTG ACGCCCACCG
3421     AGGCCAGGCG GCGCGGTGCC TTCCGTGAGG ACGCATTGAC CGAGGCCGAC GCCCTGGCGG
3481     CCGCCGAGAA TGAACGCCAA GAGGAACAAG CATGAAACCG CACCAGGACG GCCAGGACGA
3541     ACCGTTTTTC ATTACCGAAG AGATCGAGGC GGAGATGATC GCGGCCGGGT ACGTGTTCGA
3601     GCCGCCCGCG CACGTCTCAA CCGTGCGGCT GCATGAAATC CTGGCCGGTT TGTCTGATGC
3661     CAAGCTGGCG GCCTGGCCGG CCAGCTTGGC CGCTGAAGAA ACCGAGCGCC GCCGTCTAAA
3721     AAGGTGATGT GTATTTGAGT AAAACAGCTT GCGTCATGCG GTCGCTGCGT ATATGATGCG
3781     ATGAGTAAAT AAACAAATAC GCAAGGGGAA CGCATGAAGG TTATCGCTGT ACTTAACCAG
3841     AAAGGCGGGT CAGGCAAGAC GACCATCGCA ACCCATCTAG CCCGCGCCCT GCAACTCGCC
3901     GGGGCCGATG TTCTGTTAGT CGATTCCGAT CCCCAGGGCA GTGCCCGCGA TTGGGCGGCC
3961     GTGCGGGAAG ATCAACCGCT AACCGTTGTC GGCATCGACC GCCCGACGAT TGACCGCGAC
4021     GTGAAGGCCA TCGGCCGGCG CGACTTCGTA GTGATCGACG GAGCGCCCCA GGCGGCGGAC
4081     TTGGCTGTGT CCGCGATCAA GGCAGCCGAC TTCGTGCTGA TTCCGGTGCA GCCAAGCCCT
4141     TACGACATAT GGGCCACCGC CGACCTGGTG GAGCTGGTTA AGCAGCGCAT TGAGGTCACG
4201     GATGGAAGGC TACAAGCGGC CTTTGTCGTG TCGCGGGCGA TCAAAGGCAC GCGCATCGGC
4261     GGTGAGGTTG CCGAGGCGCT GGCCGGGTAC GAGCTGCCCA TTCTTGAGTC CCGTATCACG
4321     CAGCGCGTGA GCTACCCAGG CACTGCCGCC GCCGGCACAA CCGTTCTTGA ATCAGAACCC
4381     GAGGGCGACG CTGCCCGCGA GGTCCAGGCG CTGGCCGCTG AAATTAAATC AAAACTCATT
4441     TGAGTTAATG AGGTAAAGAG AAAATGAGCA AAAGCACAAA CACGCTAAGT GCCGGCCGTC
4501     CGAGCGCACG CAGCAGCAAG GCTGCAACGT TGGCCAGCCT GGCAGACACG CCAGCCATGA
4561     AGCGGGTCAA CTTTCAGTTG CCGGCGGAGG ATCACACCAA GCTGAAGATG TACGCGGTAC
4621     GCCAAGGCAA GACCATTACC GAGCTGCTAT CTGAATACAT CGCGCAGCTA CCAGAGTAAA
4681     TGAGCAAATG AATAAATGAG TAGATGAATT TTAGCGGCTA AAGGAGGCGG CATGGAAAAT
4741     CAAGAACAAC CAGGCACCGA CGCCGTGGAA TGCCCCATGT GTGGAGGAAC GGGCGGTTGG
4801     CCAGGCGTAA GCGGCTGGGT TGTCTGCCGG CCCTGCAATG GCACTGGAAC CCCCAAGCCC
4861     GAGGAATCGG CGTGACGGTC GCAAACCATC CGGCCCGGTA CAAATCGGCG CGGCGCTGGG
4921     TGATGACCTG GTGGAGAAGT TGAAGGCCGC GCAGGCCGCC CAGCGGCAAC GCATCGAGGC
4981     AGAAGCACGC CCCGGTGAAT CGTGGCAAGC GGCCGCTGAT CGAATCCGCA AAGAATCCCG
5041     GCAACCGCCG GCAGCCGGTG CGCCGTCGAT TAGGAAGCCG CCCAAGGGCG ACGAGCAACC
5101     AGATTTTTTC GTTCCGATGC TCTATGACGT GGGCACCCGC GATAGTCGCA GCATCATGGA
5161     CGTGGCCGTT TTCCGTCTGT CGAAGCGTGA CCGACGAGCT GGCGAGGTGA TCCGCTACGA
5221     GCTTCCAGAC GGGCACGTAG AGGTTTCCGC AGGGCCGGCC GGCATGGCCA GTGTGTGGGA
5281     TTACGACCTG GTACTGATGG CGGTTTCCCA TCTAACCGAA TCCATGAACC GATACCGGGA
5341     AGGGAAGGGA GACAAGCCCG GCCGCGTGTT CCGTCCACAC GTTGCGGACG TACTCAAGTT
5401     CTGCCGGCGA GCCGATGGCG GAAAGCAGAA AGACGACCTG GTAGAAACCT GCATTCGGTT
5461     AAACACCACG CACGTTGCCA TGCAGCGTAC GAAGAAGGCC AAGAACGGCC GCCTGGTGAC
5521     GGTATCCGAG GGTGAAGCCT TGATTAGCCG CTACAAGATC GTAAAGAGCG AAACCGGGCG
5581     GCCGGAGTAC ATCGAGATCG AGCTAGCTGA TTGGATGTAC CGCGAGATCA CAGAAGGCAA
5641     GAACCCGGAC GTGCTGACGG TTCACCCCGA TTACTTTTTG ATCGATCCCG GCATCGGCCG
5701     TTTTCTCTAC CGCCTGGCAC GCCGCGCCGC AGGCAAGGCA GAAGCCAGAT GGTTGTTCAA
5761     GACGATCTAC GAACGCAGTG GCAGCGCCGG AGAGTTCAAG AAGTTCTGTT TCACCGTGCG
5821     CAAGCTGATC GGGTCAAATG ACCTGCCGGA GTACGATTTG AAGGAGGAGG CGGGGCAGGC
5881     TGGCCCGATC CTAGTCATGC GCTACCGCAA CCTGATCGAG GGCGAAGCAT CCGCCGGTTC
5941     CTAATGTACG GAGCAGATGC TAGGGCAAAT TGCCCTAGCA GGGGAAAAAG GTCGAAAAGG
6001     TCTCTTTCCT GTGGATAGCA CGTACATTGG GAACCCAAAG CCGTACATTG GGAACCGGAA
6061     CCCGTACATT GGGAACCCAA AGCCGTACAT TGGGAACCGG TCACACATGT AAGTGACTGA
6121     TATAAAAGAG AAAAAAGGCG ATTTTTCCGC CTAAAACTCT TTAAAACTTA TTAAAACTCT
6181     TAAAACCCGC CTGGCCTGTG CATAACTGTC TGGCCAGCGC ACAGCCGAAG AGCTGCAAAA
6241     AGCGCCTACC CTTCGGTCGC TGCGCTCCCT ACGCCCCGCC GCTTCGCGTC GGCCTATCGC
6301     GGCCGCTGGC CGCTCAAAAA TGGCTGGCCT ACGGCCAGGC AATCTACCAG GGCGCGGACA
6361     AGCCGCGCCG TCGCCACTCG ACCGCCGGCG CCCACATCAA GGCACCCTGC CTCGCGCGTT
6421     TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC
6481     TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT
6541     GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG AGTGTATACT GGCTTAACTA
6601     TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG
6661     ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
6721     GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
6781     ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
6841     CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
6901     GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
6961     CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
7021     CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
7081     TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
7141     CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
7201     ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
7261     AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT
7321     ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
7381     ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
7441     GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
7501     GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGCAT TCTAGGTACT AAAACAATTC
7561     ATCCAGTAAA ATATAATATT TTATTTTCTC CCAATCAGGC TTGATCCCCA GTAAGTCAAA
7621     AAATAGCTCG ACATACTGTT CTTCCCCGAT ATCCTCCCTG ATCGACCGGA CGCAGAAGGC
7681     AATGTCATAC CACTTGTCCG CCCTGCCGCT TCTCCCAAGA TCAATAAAGC CACTTACTTT
7741     GCCATCTTTC ACAAAGATGT TGCTGTCTCC CAGGTCGCCG TGGGAAAAGA CAAGTTCCTC
7801     TTCGGGCTTT TCCGTCTTTA AAAAATCATA CAGCTCGCGC GGATCTTTAA ATGGAGTGTC
7861     TTCTTCCCAG TTTTCGCAAT CCACATCGGC CAGATCGTTA TTCAGTAAGT AATCCAATTC
7921     GGCTAAGCGG CTGTCTAAGC TATTCGTATA GGGACAATCC GATATGTCGA TGGAGTGAAA
7981     GAGCCTGATG CACTCCGCAT ACAGCTCGAT AATCTTTTCA GGGCTTTGTT CATCTTCATA
8041     CTCTTCCGAG CAAAGGACGC CATCGGCCTC ACTCATGAGC AGATTGCTCC AGCCATCATG
8101     CCGTTCAAAG TGCAGGACCT TTGGAACAGG CAGCTTTCCT TCCAGCCATA GCATCATGTC
8161     CTTTTCCCGT TCCACATCAT AGGTGGTCCC TTTATACCGG CTGTCCGTCA TTTTTAAATA
8221     TAGGTTTTCA TTTTCTCCCA CCAGCTTATA TACCTTAGCA GGAGACATTC CTTCCGTATC
8281     TTTTACGCAG CGGTATTTTT CGATCAGTTT TTTCAATTCC GGTGATATTC TCATTTTAGC
8341     CATTTATTAT TTCCTTCCTC TTTTCTACAG TATTTAAAGA TACCCCAAGA AGCTAATTAT
8401     AACAAGACGA ACTCCAATTC ACTGTTCCTT GCATTCTAAA ACCTTAAATA CCAGAAAACA
8461     GCTTTTTCAA AGTTGTTTTC AAAGTTGGCG TATAACATAG TATCGACGGA GCCGATTTTG
8521     AAACCGCGGT GATCACAGGC AGCAACGCTC TGTCATCGTT ACAATCAACA TGCTACCCTC
8581     CGCGAGATCA TCCGTGTTTC AAACCCGGCA GCTTAGTTGC CGTTCTTCCG AATAGCATCG
8641     GTAACATGAG CAAAGTCTGC CGCCTTACAA CGGCTCTCCC GCTGACGCCG TCCCGGACTG
8701     ATGGGCTGCC TGTATCGAGT GGTGATTTTG TGCCGAGCTG CCGGTCGGGG AGCTGTTGGC
8761     TGGCTGGTGG CAGGATATAT TGTGGTGTAA ACAAATTGAC GCTTAGACAA CTTAATAACA
8821     CATTGCGGAC GTTTTTAATG TACTGAATTA ACGCCGAATT AATTCCTAGG CCACCATGTT
8881     GGGCCCGGCG CGCCAAGCTT GCATGCCTGC AGGTCAACAT GGTGGAGCAC GACACTCTCG
8941     TCTACTCCAA GAATATCAAA GATACAGTCT CAGAAGACCA GAGGGCTATT GAGACTTTTC
9001     AACAAAGGGT AATATCGGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTCA
9061     TCGAAAGGAC AGTAGAAAAG GAAGATGGCT TCTACAAATG CCATCATTGC GATAAAGGAA
9121     AGGCTATCGT TCAAGATGCC TCTACCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA
9181     GGAACATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG
9241     ATGGTCAACA TGGTGGAGCA CGACACTCTC GTCTACTCCA AGAATATCAA AGATACAGTC
9301     TCAGAAGACC AGAGGGCTAT TGAGACTTTT CAACAAAGGG TAATATCGGG AAACCTCCTC
9361     GGATTCCATT GCCCAGCTAT CTGTCACTTC ATCGAAAGGA CAGTAGAAAA GGAAGATGGC
9421     TTCTACAAAT GCCATCATTG CGATAAAGGA AAGGCTATCG TTCAAGATGC CTCTACCGAC
9481     AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAACATCG TGGAAAAAGA AGACGTTCCA
9541     ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA
9601     CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG
9661     AGG