Sigma B5002 500MG- Brdu,5-bromo-2—deoxyuridine,5-溴脱氧尿嘧啶核苷_详细资料

产品信息

Brdu中文名称：5-溴脱氧尿嘧啶核苷英文名: Brdu ,5-bromo-2—deoxyuridine分子式：C9H11BrN2O5分子量：307.10外观：白色结晶粉末Brdu，中文全名5-溴脱氧尿嘧啶核苷，为胸腺嘧啶的衍生物，可代替胸腺嘧啶在DNA合成期(S期)，活体注射或细胞培养加入，而后利用抗Brdu单克隆抗体，ICC染色，显示增殖细胞。同时结合其它细胞标记物，双重染色，可判断增殖细胞的种类，增殖速度，对研究细胞动力学有重要意义。EdU是一种胸腺嘧啶核苷类似物，能够在细胞增殖时期代替胸腺嘧啶(T)渗入正在复制的DNA分子，通过基于EdU与Apollo荧光染料的特异性反应检测DNA复制活性，通过检测EdU标记便能准确地反映细胞的增殖情况。与BrdU检测方法相比，EdU检测方法更快速、更灵敏、更准确。EdU检测染料只有BrdU抗体大小的1/500，在细胞内很容易扩散，无需DNA变性(酸解、热解、酶解等)即可有效检测，可有效避免样品损伤，在细胞和组织水平能更准确地反映细胞增殖等现象。而BrdU需要变性DNA后才能与抗体结合，破坏了DNA双链结构，影响了Hochest等染色，导致染色弥散，准确性降低等问题，逐渐被EdU取代.Sigma相关产品资料：Sigma B5002 5-Bromo-2′-deoxyuridine ≥99% (HPLC)

Synonym:

5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR

CAS Number:

59-14-3

Linear Formula:

C₉H₁₁BrN₂O₅

Molecular Weight:

307.10

Beilstein Registry Number:

30395

EC Number:

200-415-9

MDL number:

MFCD00006529

PubChem Substance ID:

24278270

Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

性质

assay

≥99% (HPLC)

mp

191-194 °C (dec.)(lit.)

storage temp.

−20°C

安全性

Personal Protective Equipment

Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

WGK Germany

2

RTECS

YU7350000

品名

产地

货号

规格

单价

备注

Brdu5-溴-2-脱氧脲苷

Sigma

B5002

100MG

询价

现货

Brdu5-溴-2-脱氧脲苷

Sigma

B5002

250MG

询价

现货

Brdu5-溴-2-脱氧脲苷

Sigma

B5002

500MG

询价

现货

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Sigma B5002 500MG- Brdu,5-bromo-2—deoxyuridine,5-溴脱氧尿嘧啶核苷

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Synonym:	5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
CAS Number:	59-14-3
Linear Formula:	C₉H₁₁BrN₂O₅
Molecular Weight:	307.10
Beilstein Registry Number:	30395
EC Number:	200-415-9
MDL number:	MFCD00006529
PubChem Substance ID:	24278270

Frequently Asked Questions	Live Chat and Frequently Asked Questions are available for this Product.
Biochem/physiol Actions	Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

assay	≥99% (HPLC)
mp	191-194 °C (dec.)(lit.)
storage temp.	−20°C

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany	2
RTECS	YU7350000

品名	产地	货号	规格	单价	备注
Brdu5-溴-2-脱氧脲苷	Sigma	B5002	100MG	询价	现货
Brdu5-溴-2-脱氧脲苷	Sigma	B5002	250MG	询价	现货
Brdu5-溴-2-脱氧脲苷	Sigma	B5002	500MG	询价	现货