RAW264.7细胞 小鼠单核巨噬细胞系更多培养技术指导 货号:GD-C19819722
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞 全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
养了几年细胞 说一点自己培养细胞方面的看法:1)培养细胞前,可以看看细胞特点说明书,包括细胞正常生长的状态图、传代、培养基的类型等;2)不同细胞生长周期不同,有的生长很快,比如说MDA-MB-231,传代的时候取离心悬液的十分之一就已经足够了,有的又是特别慢,等的人心焦,BT474就是,传代是一分二,复苏起始的时候两周多才能长满,所以就要在培养细胞的过程中多多摸索了;3)对于娇弱的细胞 就得细心呵护了,胰酶消化不能过久,吹得时候要轻柔,离心时候也要注意转速和时间,每天都要去看一下细胞 肉眼观察培养基状况、显微镜下观察细胞生长状况;4)冻存细胞复苏后第二天Z好更换一次培养基;5)培养箱隔一段时间彻底用酒精棉擦洗一次;6)养细胞的教训:不能偷懒!细胞虐我千百遍,我待细胞如初恋。1)不管买的细胞、惠赠的细胞 刚拿到手赶紧做两件事:检测是否有支原体污染; 迅速扩增冻存;2)发现有污染迅速处理掉,特别是当你养了很多种细胞时; 细菌污染,真菌污染很容易发现,支原体污染的话,细胞会长的慢,买个支原体检测的kit定期检测一下;3)传代细胞日常的值日清洁是必须的,此外还要定期熏蒸;4)细胞的传代一定要规律,保持相同的confluency和传代时间间隔,不要随心所欲或者拖延;5)注意个人卫生。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
SodiuM arsenate dibasic heptahydrate
3-BroMo-5-fluorobenzoic Acid
4-(2-(Piperidin-1-yl)ethylcarbaMoyl)phenylboronic acid, HCl
2-Methyloctadecanoic acid
3,5-Difluoro-4-forMylbenzonitrile
5-Ethyl-2-Methylpyridine
1,9-Bis(acryloyloxy)nonane (stabilized with MEHQ)
Polyethylene Glycol Monooleyl Ether n(=:)10
Boron Trichloride - Methanol Reagent (5-10%) [for Esterification] (1ml*10)
2-Bromo-4,5-difluorophenol 97%
3-Cyanopropyldiisopropylchlorosilane
2-Chloro-4-fluorotoluene
2-Amino-3-bromo-5-nitropyridine
4'-Chloropropiophenone
5-(2-Pyridyl)thiophene-2-sulfonyl chloride
2-Thiopheneboronic acid (contains varying aMounts of Anhydride)
N-(2,6-DiMethylphenyl)piperidine-2-carboxaMide
4-(DifluoroMethoxy)-3-Methoxybenzaldehyde
2',4'-Difluoroacetophenone, 99%
1,2-EPOXYBUTANE
3-Methyl-3-heptanol
Tri-n-butylphosphine
2-(Methylmercapto)benzimidazole
N-butylphthalimide
Toldimphos sodium
(N-METHYL-N-PHENYLAMINO)TRIPHENYLPHOSPHONIUM IODIDE
2-Bromobenzenesulphonyl chloride
4-BroMo-5-Nitrothiophene-2-Carboxaldehyde
Perfluoro(2-Methyl-3-oxahexanoyl) fluoride, 97%
Bis(3-fluorophenyl) disulfide, 97%
Di(1-adaMantyl)benzylphosphine
Benzyl 2-acetaMido-2-deoxy-3-O-β-D-galactopyranosyl-α-D-galactopyranoside
4-Chloro-5-nitrophthaliMide
Neopentyl chloride, 98%
3-Methoxybenzoic acid
Nω-(4-Methoxy-2,3,6-triMethylbenzenesulfonyl)-L-arginine
3-nitro-1H-pyrazole(SALTDATA: FREE)
4-n-Butylphenylhydrazine hydrochloride
Ethyl phenylacetate ,99%
3-Fluoro-5-(propylcarbamoyl)benzeneboronic acid 98%
4-Nitro-o-phenylenediamine
1-Iodobutane
MAGNESIUM HYDROGEN PHOSPHATE TRIHYDRATE
TRI-POTASSIUM PHOSPHATE MONOHYDRATE
N-tert-Butyl 5-BroMo-2-thiophenesulfonaMide
4'-Acetoxyacetanilide
1-[(4-Nitrophenyl)Methyl]pyrrolidine
3,5-Dichlorobenzoyl chloride, 97%
2-Acetoxyphenylboronic acid pinacol ester, 97%
D(-)-Asparagine monohydrate
3-BroMoisobutyric Acid
Dibutyl phthalate
4-[2-(trans-4-Propylcyclohexyl)ethyl]cyclohexanone
Disperse Red 146
1-Acetyl-5-nitroindoline
1-Naphthylboronic acid
1,1-DiMethoxyoctane
2-AMINO-3-CHLORO-1,4-NAPHTHOQUINONE
2-FLUORO-4-(TRIFLUOROMETHYL)BENZOIC ACID
Fmoc-Lys(2-Cl-Z)-OH
4'-Hydroxychalcone, 98%
N-Chloroacetylglycine
D-Glucose-6-phosphate Barium Salt
(R)-1-Benzyl-3-N-Boc-aminomethyl pyrrolidine
5-BROMOVERATRALDEHYDE
MYRISTIC ACID N-BUTYL ESTER
3-Bromophenol
N-(2-AMinoethyl)piperidine
1-Phenyl-1H-pyrazole-5-carboxylic acid
Dicyclopentyl(diMethoxy)silane
1,5-DiMethyl-1h-pyrazole-4-carbaldehyde
(1'S,3S)-1-(1'-Phenylethyl)-5-oxo-3-pyrrolidine carboxylic acid, 98%
1-CHLORO-3-FLUOROBENZENE
6-O-(Triisopropylsilyl)-D-galactal
4-Pentenoic acid, 4-methyl-
3-Coumaranone
Ethanesulfonamide
5-Hydroxy-2-methylbenzoic acid
HSP-70 ELISA Kit
Flt3L ELISA Kit
Human hnRNP C ELISA Kit
DRD2 ELISA Kit
cGMP ELISA Kit
IL-13 ELISA Kit
Chicken major histocompatibility complex,MHC/B ELISA Kit
Human PR3 Ab-IgG ELISA Kit
Human PDGF-CC ELISA Kit
TBA ELISA Kit
Semi-solid Medium
Semi-solid Medium
2-Phenylpropyl acetate
PotassiuM trans-^b-styryltrifluoroborate, 98%
1-n-Butyl-4-chlorobenzene, 97%
2-PhenoxyethylaMine
Tri-n-butylphosphine
Pyridine, 3-ethoxy-
4-Amino-1-butanol
1-(biphenyl-4-yl)-4,4,4-trifluorobutane-1,3-dione
2-(2-Hydroxyphenyl)benzothiazole
3,3'-DIPROPYLOXACARBOCYANINE IODIDE
4,6-Dihydroxy-2-MethylpyriMidine
4-(Ethoxycarbonyl)phenylzinc iodide, 0.5M in THF, packaged under Argon in resealable CheMSeal^t bottles
1-Chloro-4-(difluoroMethyl)benzene, 97%
2,3-Diphenylquinoxaline
1-Methyl-2-pyrrolecarboxylic Acid
2-BroMoMethyl-4-trifluoroMethoxyphenylboronic acid, pinacol ester
Mordant Black 3
(4-BroMo-3-Methylphenylcarbonyl)pyrrolidine
4-(CHLOROMETHYL)PHENYLTRICHLOROSILANE
8-Fluoro-4-hydroxy-2-methylquinoline
methyl 2-amino-3,5-dichlorobenzoate(SALTDATA: FREE)
1,1,1,2,2-Pentafluoropropane 97%
Tin(II) chloride, anhydrous, 98%