U-937细胞 人淋巴瘤细胞系培养技术经验更多 货号:GD-C19119134
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞 全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞 其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-AMinobenzenesulfonaMide, tech. 80%
4-PiperidineMethanol
1,1,2,3,3-PENTACHLOROPROPANE
PENTACHLOROTHIOPHENOL
2,4-DIMETHYL-6-NITROPHENOL
1-BroMo-4-(MethoxyMethoxy)benzene
(R)-(+)-(3-Chlorophenyl)oxirane
Ethyldiphenylphosphine, 99%
2,2'-Dihydroxydiphenylmethane
(+/-)-Limonene
(Chloromethyl)dimethylvinylsilane(Vinyl(chloromethyl)dimethylsilane)
Tetraphenylmethane
N-Methyl-L-prolinol
3-FLUORO-L-PHENYLALANINE
PotassiuM pyrosulfate
Benzanilide
3,5-Dichloro-2,4-difluoroaniline
Undecafluorohexanoyl Fluoride
Tris(4-broMophenyl)aMiniuM hexachloroantiMonate, 95%
SeleniuM Sulfide, USP
Diisodecyl 4-Cyclohexene-1,2-dicarboxylate
N-propylaniline
Pigment Red 2
ethambutol dihydrochloride
2-Fluoronicotinic acid
2-CHLOROPHENYL ISOTHIOCYANATE
BENZYLTRIETHYLAMMONIUM BOROHYDRIDE
Silicotungstic Acid Hydrate
3-Isobutylpyridine, 99%
2-Fluoro-6-hydroxypyridine, 97%
AluMinuM sulfate
N,N,N′,N′-TetraMethylethylenediaMine
2,5-Diphenylfuran, 98%
PHENYLBIGUANIDE
METHYL 3-BROMO-4-METHOXYBENZOATE 98
TaMsulosin hydrochloride
3-Bromo-4-(trifluoromethoxy)aniline
3,4-Difluorobenzenesulfonyl Chloride
Bis(hexafluoroacetylacetonato)manganese (II)
Triethylene Glycol Monododecyl Ether
DL-2-AMINOOCTANOIC ACID
4-Phenoxybutanoic acid
N-ACETYL-D-PHENYLALANINE
Dimethylamine
2-Benzyloxybenzeneboronic acid(contains varying aMounts of Anhydride)
Benzene-d6
4-Iodo-1-Methylpyrazole-5-carboxylic Acid
4-Fluoro-2-nitro-1-(trifluoroMethoxy)benzene
2-Methyl-2-vinyloxirane, 95%
Betamethasone 21-acetate
cis-1,2-Cyclohexanedicarboxylic Anhydride
2-Chlorobenzenesulfonamide
2-Ethoxybenzhydrazide
Mordant yellow 8 (C.I. 18821)
sodium 1,2-bis(tridecyloxycarbonyl)ethanesulphonate
2-BROMOBENZYL CHLORIDE
Losartan potassiuM
2-CHLOROBENZYL ISOTHIOCYANATE
3-(t-ButyldiMethylsilyloxy)-5-Methylphenylboronic acid, pinacol ester
Polyethylene Glycol 400
N-(2-Hydroxyethyl)phthalimide
1,1,3,5,5-Pentaphenyl-1,3,5-trimethyltrisiloxane
(S)-(+)-2-(Aminomethyl)pyrrolidine, 98%
1,4-Pentadien-3-ol
Bromochlorophenol Blue
3-Cyclopentene-1-carboxylic acid
N,N-DIMETHYLFORMAMIDE-D7
6-AMino-3-nitro-2-picoline
1-AMino-2-naphthol hydrochloride
4-Isopropyl-3-thioseMicarbazide
5-BroMo-3-fluorobenzene-1,2-diaMine
Methyl phenoxyacetate, 99%
4-CHROMANOL
Muco-Inositol
3,4-Dimethylbenzaldehyde
5-Hexyn-3-ol
6-bromo-2-naphthoic acid
Pyrrolidine-2-thione
DPD ELISA Kit
RELA ELISA Kit
Human Apolipoprotein E,Apo-E ELISA Kit
Human DEFβ128 ELISA Kit
EGF ELISA Kit
Rat FOS (V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog) ELISA Kit
Chicken prolactin,PRL ELISA Kit
Human Bullous Pemphigoid,BP ELISA Kit
Human ANG-R-Tie2 (Angiopoietin Receptor Tie2) ELISA Kit
SDPR ELISA Kit
Mycoplasma Medium,Fluid
Mueller-Hinton Agar
Isopropyl-^b-D-thiogalactoside, dioxane-free, 99%
GadoliniuM
5-Chloro-2,4-Difluorobenzonitrile
5-BroMo-2-Fluoronicotinic Acid
N-propylaniline
Reactive Blue 49
glutathione peroxidase F. bov. erythro-cytes
DL-Homocysteine
3,5-DI-TERT-BUTYL-4-HYDROXYPHENYLACETONITRILE
BIS(2-ETHYLHEXYL)PHTHALATE (RING-D4)
5-BroMo-2-Chloroisonicotinic Acid
3-Decanol, 97%
2,3-O-Isopropylidene-1-O-Methyl-D-ribosic acid, 97%
Benzyloxyacetic acid
4-Ethylpyridine
Fluoroacetone
Benzene-1,4-diboronic acid Mono-MIDA ester
2-BroMo-1-(4-(piperidin-1-yl)phenyl)ethanone
Tetrakis(decyl)aMMoniuM hydroxide solution
5-chloro-2-(1H-1,2,4-triazol-1-yl)aniline(SALTDATA: FREE)
Butyl Trifluoromethanesulfonate
n-Undecylamine, 98%
Cefaclor