LM-6细胞系—人肝癌细胞系全国减价 货号:GD-C16819665
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Silver hexafluoroantiMonate(V)
Z-Ligustilide
8-BroMoquinolin-2(1H)-one
3-Chloro-4-(N-isopropylcarbaMoyl)phenylboronic acid
1,3-DIAMINOPROPANE-N,N'-DIACETIC ACID
3-Butoxyphenylboronic acid
4,4'-Bismaleimidodiphenylmethane
Benzyl 4-hydroxybenzoate
2-Trimethylsilylanisole, 98%
Methyl perfluoroundecanoate
N-(4-Methylphenyl)-3-oxobutanamide
1,4-Bis(methoxymethyl)benzene
Benzyl thiocyanate
1,12-Dodecanediamine
Methyltrichlorosilane
3,4-Dichloropyridine
Ethyl (R)-(+)-1-Ethyl-2-pyrrolidinecarboxylate
2-[4-(TrifluoroMethyl)phenyl]benzaldehyde
2-BroMothiazole-4-carboxaldehyde, 97%
CHLORPYRIFOS
Benzyl DiMethylphosphonoacetate
Vitamin E
Ammonium iron (II) sulfate hexahydrate
4-Chlorophenylsulfonylacetonitrile
3-Formylbenzoic acid
4-Methylbenzophenone
(R)-5-Hydroxymethyldihydrofuran-2-one
2,3,5-Trichloropyridine
Triethyl orthoforMate, 98%
3-BroMo-2-chlorothiophene, 97%
3-(FurfurylaMinocarbonyl)benzeneboronic acid pinacol ester, 97%
trans-3,5-DifluorocinnaMic Acid
N-Methyl-1,3-propanesultaM
N,N-Diethyl 4-broMonaphthaMide
2,6-DIBROMO-4-CHLORO-3,5-DIMETHYLPHENOL
Dicyclohexyl phthalate
beta-Ionone, synthetic, 96%
2,5-Difluorophenyl isocyanate, 98%
Pravastatin Sodium
1,3,5,7-Tetramethyl-6-phenyl-2,4,8-trioxa-6-phosphaadamantane, 99%
Vanadyl phthalocyanine, pract., 85%
4-FLUORO-2-(TRIFLUOROMETHYL)BENZYL ALCOHOL
2-Bromo-6-nitrophenol
Diethyl succinate
4-Phenyl-1-butanol
2-AdaMantanone
1-(2,4-Dinitrophenyl)pyridiniuM Chloride
N-Pentyl 3-broMophenylsulfonaMide
Bicyclo[3.3.1]nonane-2,6-dione, 97%
TranexaMic Acid, EP
1-MethyliMidazole
2-oxo-4-phenylbutyric acid
3,3'-iminobis(N,N-dimethylpropylamine)
Divinyl Benzene
m-Anisidine
Amidinothiourea
Methyl Green zinc chloride salt
6-(3-Thienyl)pyridine-2-carboxaldehyde
1,3-DibroMopropane
1-Bromoeicosane
2-(2,6-Dichlorophenyl)ethanol
tert-Butyl Propionate
Congo Red
4-Methoxy-2-aminobenzothiazole
2-CHLORO-5-NITROANISOLE
2,3-DIFLUOROMANDELIC ACID
TorseMide Related CoMpound B;N-[(n-butylaMino)carbonyl]-4-[(3-Methylphenyl)aMino]-3-pyridinesulfonaMide
2-Fluorophenylboronic acid pinacol ester
2,2-Bis(4-hydroxyphenyl)hexafluoropropane
7-BroMoindole-2-boronic acid
6-Methylnicotinic acid, 99%
1-Phenyl-5-(trifluoroMethyl)-1H-pyrazole-4-carboxylic acid, 97%
Glyoxal SodiuM Bisulfite Hydrate
Guanylurea Sulfate
3-Nitrophenol
N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate
Direct Red 243
3-(benzylsulfinyl)alanine
uPA ELISA Kit
BMPER ELISA Kit
PINS ELISA Kit
Human low-density lipoprotein-receptor-related protein,LRP-6 ELISA Kit
TSGF ELISA Kit
IL-9 ELISA Kit
Mn-SOD/SOD2 ELISA Kit
Human M-AChR M2 (Muscarinic Acetylcholine Receptor M2) ELISA Kit
IAPP ELISA Kit
CⅠCP ELISA Kit
Brucella vaccine culture medium (liquid)
ISP Medium 2(Yeast Extract Malt Extract Agar)
Ethyl 2-MethyliMidazo[1,2-a]pyridine-3-carboxylate, tech. 90%
trans-3-HydroxycinnaMic acid, 99%
4-Vinylbenzyl Chloride
2-AMinopyridine
Vitamin E
Thiophene-2-Sulfonyl Chloride
oct-4-enedioic acid
diphenyl succinate
Ethyl isobutyrylacetate
6-Bromomethyl-3,4-dihydro-2-methyl-quinazolin-4-one
Adipic Acid Diethyl Ester
3-Chloro-4-fluorobenzyl broMide, 97%
4-Methylstyrene, 98+%, stab. with 0.1% 3,5-di-tert-butylcatechol
4-Iodo-1H-iMidazole
DeMeton-S-Methyl
2-N-BUTOXYPYRIDINE
2-HYDRAZINOQUINOLINE
2-(2-PYRIDYLAZO)-5-DIMETHYLAMINOPHENOL
1,2-Epoxyhexadecane , 80%
Ethyl 1-methyl-5-(trifluoromethyl)-1H-pyrazole-4-carboxylate
5-Amino-3-[3-(trifluoromethyl)phenyl]isoxazole
1-Adamantyl bromomethyl ketone
1-(2-Methoxyphenoxy)-2-propanol