H-97细胞系—人高转移肝癌细胞系全国减价 货号:GD-C16819594
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Diethyl Maleate
Neochlorogenic acid
Fyrol FR 2
4-PROPIONYLOXYBENZALDEHYDE
Ethyl 6,7-dichloro-3-trifluoroMethyl-1,4-dihydroquinoxaline-2-carboxylate
SODIUM HEPTAFLUOROBUTYRATE
5-(2-Chloro-phenyl)-2H-pyrazole-3-carboxylic acid
7-bromo-5-fluoro-1H-indole-2,3-dione(SALTDATA: FREE)
2-chloro-N-cyclohexyl-N-methylacetamide(SALTDATA: FREE)
Chrysoidin, pure
Tetrachlorvinphos
(R)-(+)-2-Tetrahydrofuroic acid
Nitrocellulose
2-Hydroxy-5-methyl-3-nitropyridine
Benzophenone IMine
(R)-(-)-TetrahydrofurfurylaMine
1,2-Epoxyhexadecane
Isoquinoline-3-carbaldehyde
5-BroMo-2-hydroxy-3-Methoxybenzaldehyde, 97%
AMETRYNE
2-Methylundecane
copper dioleate
Calcium sulfate
2-n-Propylaniline
ethyl 2-benzoylbenzoate
1-AMINO-1-CYCLOHEXANECARBOXYLIC ACID HYDROCHLORIDE
GERANYL CHLORIDE
2-AMino-5-BroMopyriMidine
2-Fluoro-5-Methoxybenzonitrile, 98%
1-Ethynyl-2,2,6-triMethylcyclohexanol, (E)+(Z), 97%
4,6-DiMethyl-2-pyriMidinethiol
Terbutol
4-Hydroxyphenylboronic acid pinacol ester
N-AcetylethylenediaMine
4-FLUORO-3-(TRIFLUOROMETHYL)PHENYLACETIC ACID
1-HYDROXY-2-NAPHTHALDEHYDE
2-(2-Hydroxyphenyl)acetic acid, 97%
4-Benzyloxy-3-methylphenylboronic acid
1,2,3,4,5,6,7,8-Octahydroacridine
2-amino-4-ethyl-5-methylthiophene-3-carboxamide(SALTDATA: FREE)
Rhodium(III) oxide pentahydrate
3,5,5-TRIMETHYLOXAZOLIDINE-2,4-DIONE
Trifluoroacetic anhydride
(S)-(-)-N-Benzyl-1-phenylethylamine
3-AMino-4-chloropyridine
Butylated hydroxyanisole
9-Ethylcarbazole-3-carboxaldehyde N-Benzyl-N-phenylhydrazone
3-AcetylaMino-adaMantane-1-carboxylic acid
PotassiuM, 98%, chunks, in Mineral oil
DIFLUBENZURON
3,5-DiMethylbenzenethiol
1-Triphenylphosphoranylidene-2-propanone
Catalase From Micrococcus Lysodeikticus
3-Chlorothiophenol
Ethyl 2-benzylacetoacetate
2,5-Dichlorobenzaldehyde
4-(FurfurylaMinocarbonyl)phenylboronic acid
SEC-BUTYLMAGNESIUM CHLORIDE
2,3,5,6-TETRAFLUOROBENZOTRIFLUORIDE
1-Methyl-1H-pyrazole-4-carboxylic acid
N~1~,N~1~-diethyl-1-(2-furyl)ethane-1,2-diamine(SALTDATA: FREE)
(3,5,6-trimethylpyrazin-2-yl)methanol(SALTDATA: FREE)
tert-Butyl 6-cyano-1,2,3,4-tetrahydroisoquinoline-2-carboxylate
Diallyl phthalate
3-Carbamoymethyl-5-methylhexanoic acid
1-CYCLOHEXENYL ACETATE
4-METHOXY-3-BUTEN-2-ONE
Valerophenone
2-(Styrylthio)acetic acid
4-AMino-2-diMethylaMino-6-hydroxy-5-nitrosopyriMidine [for DeterMination of Co(III), Fe(II)]
Boc-asp(otbu)-osu
FuMaraMide, 98%
Titanium dioxide
1,4-Bis(diphenylphosphino)butane
piperidine-1-acetonitrile
triangulo-dodecacarbonyltriosmium
5-Methyl tetrazole
calcium silicate
PRR4 ELISA Kit
CD3 ELISA Kit
Human REG3α (Regenerating Islet Derived Protein 3 Alpha) ELISA Kit
Human FAS/CD95 ELISA Kit
tPA ELISA Kit
IL-2R/CD122 ELISA Kit
Nude Mouse Clara cell protein,CC16 ELISA Kit
ASC ELISA Kit
Human oxidized lowdensity lipoprotein antibody,OLAb ELISA Kit
5HT ELISA Kit
Rappaport-Vassiliadis Medium,Semisolid Modificatio
Muller Kauffmann Tetrathionate Novobiocin Broth Ba
Ethylphosphonic acid, 98+%
trans-1-(Boc-aMino)-4-(4-Methyl-1-piperazinyl)cyclohexane, 97%
4-Acetoxybenzoic acid, 98+%
2-Aminonicotinic acid
copper dioleate
cromolyn sodium
N-(tert-Butoxy carbonyl)-L-valine
3,5-Dinitrotoluene
2-AMINO-4-ANILINO-1,3,5-TRIAZINE HYDROCHLORIDE
2,6-Dimethyl
3-(Propoxycarbonyl)Benzeneboronic Acid 97%
NicotinaMidoxiMe, 97%
O-Acetylsalicyloyl chloride, 97%
Thisosalicyilic acid
NiobiuM pentaoxide
2-(3-Methyl-3-nitrobutyl)-1,3-dioxolane
5-Chloro-2-nitroanisole
(-)-COTININE
Ethyl 4-hydroxypiperidine-1-carboxylate
2-Amino-4,5,6,7-tetrahydro-1,3-benzothiazole
Calcium bis(2,5-dihydroxybenzenesulfonate)
2,4,6-Trivinylcyclotriboroxane pyridine complex
1-(3-Methoxyphenyl)ethanamine