HEF细胞系—人胚纤维细胞系全国减价 货号:GD-C16819613
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3'-AMino-2',3'-dideoxyguanosine
Tris(triMethylsilyl)silane
0
5-BroMo-4-cyclopentylpyriMidine
1-Phenylvinylboronic acid MIDA ester
(4-Nitrophenyl)Methyl 3-oxobutanoate
8-Amino-1,7-naphthyridine
2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase]
4-Amino-5-methyl-4H-1,2,4-triazole-3-thiol
3-Chloro-4-formylphenylboronic acid
4-AMINO-4H-1,2,4-TRIAZOLE-3-THIOL
2-(Perfluorohexyl)ethyl methacrylate
Difenidol hydrochloride
N-HEXADECYLSULFURIC ACID SODIUM SALT
Allyl Methyl sulfide
2-BroMoMethyl-1,3-dioxolane
2-Ethylcyclohexanol (cis- and trans- Mixture)
1-(CarboxyMethyl)cyclohexane-1-carboxylic acid
4-Cyanophenylacetic acid, 97%
BENZO(GHI)PERYLENE
cis-3-Hexen-1-yl Hexanoate
2-Mercaptopyridine-N-oxide
2-Propenoic acid, 3-phenyl-, 1-methylethyl ester
Dimethylmethoxychlorosilane
2,6-Dinitroaniline
1-Piperidinepropionitrile
5-NORBORNENE-2-OL
2,6-Dichloro-4-Nitrophenol
SodiuM peroxide, 95%
1-Octen-3-one, 97%, stab. with 0.1% BHA
5-Acetyl-2-thienylboronic acid
3-AMinobiphenyl
Tris(3,3,4,4,5,5,6,6,6-nonafluorohexyl)tin broMide
N-[BIS(4-METHOXYPHENYL)METHYLENE]BENZYLAMINE
Hydrazine dihydrochloride
4-(2-Acetoxyethoxy)toluene , 98%
2-Benzylbenzyl alcohol, 97%
5,6-Difluoroindole
4-Phenyl-5-(trifluoromethyl)thiophene-2-carbonyl chloride 97%
6-Chloroimidazo[1,2-a]pyridine-2-carboxylic acid
Methyl DL-2-aminopropanoate hydrochloride
Borane-methyl sulfide complex
Fluvastatin
α-Ketoglutaric acid;2-Oxopentanedioic acid;2-Oxo-1,5-pentanedioic acid
(3-Phenylisoxazol-5-yl)MethylaMine
2,4,5-Trifluoro-3-Methoxybenzoyl chloride
4-ForMyl-trans-stilbene
5-AMino-4-cyano-1-(2,4-difluorophenyl)-3-Methyl-1H-pyrazole
ActinoMycin D, 93%
L-THIOCITRULLINE
2,2'-Dinitrodibenzyl
2-Vinylthiophene
Direct Yellow 120
diethyl (4-nitrobenzyl)phosphonate
N-(3-Hydroxyadamantan-1-yl)acetamide
(R)-(-)-1-INDANYL ISOTHIOCYANATE
DiMethyl glutaconate
2-(4-CHLOROPHENYL)OXIRANE
2-(CyclopentylaMino)pyriMidine-5-boronic acid, pinacol ester
Glucose Oxidase [from aspergillus niger, >=200 units/mg?]
3-Fluoro-3-deoxy-D-glucopyranose
6-Chloro-4-amino-2-methoxypyrimidine
3-bromo-4-chloroquinoline(SALTDATA: FREE)
Octachloronaphthalene
2-MERCAPTO-1-METHYLIMIDAZOLE
Potassium titanium oxalate
(-)-4-(4-Dimethylamino)-1-(4-fluorophenyl)-1-(hydroxybuty)-3-hydroxymethyl)-benzonitrile hemi D-(+)-di-p-toloyltartaric acid salt
2-BroMo-2-Methylpropane
2,2,2-Trifluoroacetophenone
2,4,5-Trifluorobenzyl BroMide
2-HydroxybenzylaMine, 98%
SodiuM (para)periodate, 98%
2-METHYLVALERIC ACID
1-Butyl-4-MethylpyridiniuM BroMide
tert-Butyl (5-amino-1,2-benzoxazol-3-yl)carbamate
4-IODO-2-(TRIFLUOROMETHYL)BENZONITRILE
2,4-Dichlorophenylacetyl chloride
3-Amino-2,2-dimethyl-1-propanol
Porcine Cortisol ELISA Kit
CD19 ELISA Kit
Human pancreatickininogenase,PK ELISA Kit
Human β amyloid precursor protein,βAPP ELISA Kit
TBA ELISA Kit
IL33 ELISA Kit
Aβ1-40 ELISA Kit
Human ASK1 ELISA Kit
FA ELISA Kit
6-K-PG ELISA Kit
Buffered Peptone Water
Rappaport-Vassiliadis Broth Medium, Modification
EthylMalonic acid, 98%
Trans-Ferulic Acid
ETHYL 4-ACETYLBENZOATE
Methyl 2-aminothiophene-3-carboxylate
2-Mercaptopyridine-N-oxide
7-Fluoro-4-quinazolone
DIOXOPROMETHAZINE HCl
3-Nitrobenzyl alcohol
2-Chlorobenzyl chloride
COPPER(I) CYANIDE
2-BroMo-5-Nitroaniline
Diethyl Maleate, 97%
VinyltriphenylphosphoniuM broMide, 97%
(R)-(+)-α-Methyl-1-naphthaleneMethylaMine
IridiuM(III) chloride
TRIMETHYLHEXAMETHYLENE DIISOCYANATE
2-Ethyl-2-MethacryloyloxyadaMantane (stabilized with MEHQ)
3-(2-Methoxy-3-Methylphenyl)benzoic acid
4,4'-DIHYDROXY-3,3'-DIMETHYLDIPHENYLMETHANE
4-(tert-Butyl)-1,3-thiazol-2-amine, 97%
Potassium hexachloroiridate(IV), 99%
Solanesol ,90%
1-ethyl-6-oxopiperidine-3-carboxylic acid(SALTDATA: FREE)