HCPF细胞系—人脉络丛成纤维细胞系全国减价 货号:GD-C16819609
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
1,6-Diiodohexane
IMazaquin
5-BroMo-4-Methyl-2(1H)-pyridinone
DIPHENYLIODONIUM HEXAFLUOROARSENATE
Ethyl laurate
4-Fluorophenyl sulfone
Diethyl 2-ethyl-2-(p-tolyl)malonate, 97%
Ethyl 5-nitrobenzo[b]furan-2-carboxylate
4,5-Dimethoxy-2-iodobenzoic acid
3-Phenylazo-2,6-diaminopyridine Monohydrochloride
2-AZACYCLONONANONE
CYCLOPENTANETHIOL
Triclosan
2-Bromo-3-nitrotoluene
2,4,6-Triisopropylbenzenesulfonyl Chloride
4-[3-(DiMethylaMino)propoxy]benzylaMine
(+/-)-trans-Epoxysuccinic Acid
1-Methyl-1h-benziMidazole-5-carboxaldehyde
7-(2,3-Dihydroxypropyl)theophylline, 99%
BENZO(A)PYRENE
1-Methoxy-1-triMethylsilyloxypropene
3-HYDROXY-4-METHYLPYRIDINE
Butanoic acid, 3-oxo-, 2-methylpropyl ester
Trimethylmethoxysilane
iodipamide
1,6-Dibromo-2-naphthol
DI-TERT-BUTYL ACETYLENEDICARBOXYLATE
Neopentyl Alcohol
YttriuM(III) sulfide, 99.9% (REO)
5-(2-BroMophenyl)isoxazole, 95%
Ethylene carbonate, 99%
Neopentyl glycol
10,12-Nonacosadiynoic Acid , 97%
N-2-Acetylguanine
4-tert-Butylbenzaldehyde
2,3-BIS(2,4,5-TRIMETHYL-3-THIENYL)MALEIMIDE
(1S,2S)-(+)-N-(4-Toluenesulfonyl)-1,2-diphenylethylenediaMine
Methyl cyclohexanecarboxylate
Di-s-butoxyaluminoxytriethoxysilane
Propanoyl fluoride 97%
2-2-(Triphenylmethyl)-2H-tetrazol-5-ylphenylboronic acid
Tetrakis(triphenylphosphine)platinum
Phentolamine
Benzydamine
4-Fluorobenzophenone
2-(N,N-DiMethylaMinoMethyl)phenylboronic acid
3,5-DibroMoanisole
Ethyl 8-broMo-4-hydroxyquinoline-3-carboxylate
Triethyl Methanetricarboxylate, 98%
PotassiuM Iodate, Reagent, ACS
3-BroMo-4-hydroxybenzoic Acid Hydrate
Ethyl 2-bromoisovalerate
(3,4-Dichlorobenzyl)methylamine
1,4-Diethynylbenzene
N-(4-fluoro-3-methylphenyl)-Acetamide
2,3,5-Tribromothiophene
(S)-(+)-2-DIBENZYLAMINO-3-PHENYL-1-PROPANOL
2-CHLOROETHYLUREA
D(-)-4-Hydroxyphenylglycine
Petrolatum, white, pure
trans-2,trans-4-Hexadienal, sum of isomers, 95%
Ethyl 2-Furancarboxylate
(2S,4S)-(-)-2,4-Bis(diphenylphosphino)pentane(norbornadiene)rhodium(I) hexafluorophosphate
N-HEPTYL ISOCYANATE
Trazodone
Direct Blue 71
2,3,4,6-TETRA-O-ACETYL-BETA-D-GLUCOPYRANOSYL ISOTHIOCYANATE
2,5-DiMethylbenzenesulfonic acid dihydrate
2,1,3-Benzoxadiazole-5-carboxylic acid
2-Fluoro-5-(trifluoroMethyl)benzonitrile
2-AMino-N-MethylethanesulfonaMide, HCl
2-Hydroxy-4-Methylpyridine, 97%
SUDAN RED 7B
Ethyl 4-Pyridylacetate
3-Chlorophenylhydrazine hydrochloride
1,4-bis(4-methyl-α-styryl)benzene
Tricyclohexylchlorotin
testolactone
CORT ELISA Kit
CD2 ELISA Kit
Human Pancreatic carcinoma markers-CA242 ELISA Kit
Human APAF1 ELISA Kit
TPSA ELISA Kit
Rat Interleukin 32,IL-32 ELISA Kit
PⅡNT ELISA Kit
Human FASL ELISA Kit
FA ELISA Kit
6-OHDA ELISA Kit
EF-18 Agar Base
The underlying medium (Ames test)
Ethyl Orange, 98%
DiMethyl trans-cyclohexane-1,4-dicarboxylate, 99%
4-Acetylpyridine
2-AMinothiazole, 97%
3-HYDROXY-4-METHYLPYRIDINE
4-Ethyloctanoic acid
1-methyl-2-imidazolecarboxaldehyde
4-nitrosalicylic acid
TRIMETHYLSILYL ISOTHIOCYANATE
4-Benzylaniline
4-Ethynyl-2,6-diMethyl-4-heptanol, 97%
4-TrifluoroMethylbenzoic anhydride, 97%
2-Fluoroquinoline-3-boronic acid, 97%
3,3'-Dihydroxybenzidine
3-(TRIMETHYLSILYL)-1-PROPANESULFONIC ACID SODIUM SALT
PlatinuM bis(acetylacetonate)
HAFNIUM TRIFLUOROACETYLACETONATE
Diphenyl oxide
3-AMino-2-Methylbenzyl alcohol
4-Iodobenzotrifluoride (stabilized with Copper chip)
2,8-Dimethyldibenzothiophene
1-Bromo-2,2-dimethoxypropane, 97%
4-Cyano-4-phenylcyclohexanone, 97%