COS-6细胞系—非洲绿猴肾细胞系全国减价 货号:GD-C16819572
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
AMpicillin trihydrate
2-Fluoro-6-(trifluoroMethyl)pyridine
5-Chloro-2-(trifluoroMethyl)benzoicacid
4-Chloro-DL-Mandelic Acid , 98%
D-Iditol
1,5-DiMethylpyrazole-4-boronic acid
Butyl p-Toluenesulfonate
N-(4-Iodo-2-methoxypyridin-3-yl)pivalamide
Dimethylammonium dichlorotri(?chloro)bis[(R)-(+)-2,2'-bis(di-p-tolylphosphino)-1,1'-binaphthyl]diruthenate(II) [NH2Me2][{RuCl((R)-tolbinap)}2(?Cl)3]
1-Bromo-3-(chloromethyl)-2-fluorobenzene
2-Furan-3-ylpyrrolidine
Silver oxide
METHYLENEDIPHOSPHONIC ACID
3-ACETAMIDOPHENOL
Heptylbenzene
2-AMino-3-nitro-6-Methoxypyridine
3-Ethyl-o-xylene
(2-BroMophenylcarbonyl)piperidine
4-N-BOC-2-oxo-, 97%
a,a,a-TRIFLUOROTOLUENE
Tributylphosphine
L-Arginine hydrochloride
2-Naphthylacetonitrile
cyclopentyl mercaptan
Aurin
(-)-LINALOOL
4-(FMOC-AMINO)-1-METHYL-1H-IMIDAZOLE-2-CARBOXYLIC ACID
Carbonic acid aMMoniuM zirconiuM salt
3'-(TrifluoroMethoxy)acetophenone, 97%
(R)-2-AMino-3-benzyloxy-1-propanol hydrochloride, 98+%
Z-D-Lys(Boc)-OH
Bis(1,5-cyclooctadiene)nickel(0)
3-CYANO-5-METHYLPYRIDINE
NapropaMid
3-(1H-Tetrazol-5-yl)phenol, 97%
(2-DIMETHYLAMINOETHYL)TRIPHENYLPHOSPHONIUM BROMIDE
Indium(III) oxide (99.999%-In) PURATREM
beta-Naphthol Violet
2-(Allyl)hexafluoroisopropanol
Nicotine Bi-L-(+)-tartrate Dihydrate
Butylmagnesium chloride
Acid Yellow 44
Xipamide
Ethylene Glycol;Ethylene alcohol;
tert.-Butyl Methyl Malonate
Hexyl salicylate
PhthaliMide DBU Salt
(R)-(+)-2-Methoxypropionic acid, 97%
1,7,7-TriMethylbicyclo[2.2.1]heptan-2-aMine hydrochloride, 97%
L-Lysine homopolymer hydrobromide
1-Chloroethyl Ethyl Carbonate
4'-tert-butyl-4-chlorobutyrophenone
dimethyl 4-aminothiophene-2,3-dicarboxylate
ginsenoside Rg1
5-Chloro-2-iodobenzoic acid
2',4',5'-TRIMETHYLACETOPHENONE
2-NITRO-4-(TRIFLUOROMETHYL)PHENYLACETONITRILE
4,4'-Methylenedianiline
Ethyl 2-Methylpyridine-4-carboxylate hydrochloride
5-Bromo-6-hydroxynicotinic acid
Pyrene
Diisobutylene (mixture of isomers) (stabilized with BHT)
2,3,6,7-Tetrahydro-8-hydroxy-1H,5H-benzo[i,j]quinolizine-9-carboxaldehyde
Fmoc-N-epsilon-trifluoroacetyl-L-lysine
4'-TRIFLUOROMETHYL-BIPHENYL-4-CARBALDEHYDE
ALPHA-AMINOISOBUTYRIC ACID METHYL ESTER HYDROCHLORIDE
VitaMin F
(R)-(-)-2-AMino-1-butanol
2-(ButylaMino)ethanol
5-BroMo-2,3-Methylenedioxyphenylboronic acid
8-Anilino-1-naphthalenesulfonic acid MagnesiuM salt, 95%
PyridoxaMine dihydrochloride, 98%
3,3-TetraMethyleneglutariMide
Bis(2-ethylhexyl) Hydrogen Phosphate [for Rare Metals Extraction]
1,4-Cyclohexanediol, mixture of cis and trans
1H-Indene-2-carboxylicacid, 2,3-dihydro-4-nitro-
2-(Pivaloylamino)pyridine
Transparent Orange 3g
PHLDA2 ELISA Kit
CD ELISA Kit
Human EPT1 ELISA Kit
Human Azidothymidine,AZT ELISA Kit
T ELISA Kit
Rat Interleukin 27,IL-27 ELISA Kit
VTG ELISA Kit
Human ETFβ ELISA Kit
Human FUT5 ELISA Kit
25OHVD3 ELISA Kit
Tetrathionate Broth Base
Violet Red Bile Agar with Glucose and Lactose
Ethyl cellulose
trans-2-(Boc-aMino)cyclopropaneMethanol, 97%
4-Ethoxy-N-(4-fluorobenzyl)aniline, 97%
7-Anilino-3-diethylaMino-6-Methyl fluoran
L-Arginine hydrochloride
3-METHYLENECYCLOBUTANECARBOXYLIC ACID
2,6-Dibromonaphthalene
Isatin-3-oxime
N-Hexadecyltrimethylammonium chloride
2-Pyrazinecarboxylic acid
2-Chloro-2',4'-difluoroacetophenone
3-(tert-ButylsulfaMoyl)benzeneboronic acid, 97%
Hexafluoropropene triMer, 97%
4-(TrifluoroMethyl)benzyl broMide
α-MaleiMidopropionyl-ω-succiniMidyl-10(ethylene glycol)
Dodecyl isocyanate
4,4'-DiaMino-2,2'-stilbenedisulfonic acid
2,7-Dichloro-8-Methylquinoline
2,2-DiMethyl-4-pentenoic acid
Triphosphopyridine nucleotide, sodium salt, hydrate
(R)-(+)-2-Pentanol
4-ethyl-3,5-dimethyl-1H-pyrrole-2-carbaldehyde(SALTDATA: FREE)
4(3H)-Pyrimidinone