VEROC1008(E6)细胞系—非洲绿猴肾细胞系全国减价 货号:GD-C16820578
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
4-Methyl-3-nitrobenzonitrile, 98%
Tris(pentafluorophenyl)borane
1,4-DIBROMO-2,3-BUTANEDIONE
PLUMBAGIN
4-(5-BroMopyridin-3-ylsulfonyl)Morpholine
OxaMic hydrazide
ZINC TUNGSTATE
2-Bromo-4-fluorobenzoic Acid
2-(Diethylamino)-N-(2,6-dimethylphenyl)-acetamide, 97.5%
N-Sulfinyl-p-toluenesulfonamide
4,4'-Diamyloxybiphenyl
5,5-DIMETHYL-1-PYRROLINE N-OXIDE
Fmoc-3-Aminomethylbenzoic acid
Solvent Orange 63
Amino tris(methylene phosphonic acid)
Methyl 2-aMino-3-carboxybenzoate
DiallyldiMethylsilane
5-BroMo-pyridine-2-sulfonyl chloride
SuccinaMic acid, 97%
Mercuric Nitrate, Monohydrate, Reagent, ACS
5-BroMo-5'-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl)-2,2'-bithiophene
18-Beta-Glycyrrhetinic acid
1-methylhomo
Iminodiacetonitrile
N,N'-Methylenebisacrylamide
ETHYL P-TOLYLACETATE
2-Cyanobenzyl bromide
2-Nitrobenzyl broMide
Ethyl 5-acetyl-2-ethoxybenzoate, 98%
3H-1,2-Benzodithiol-one 1,1-dioxide, 99%
3,5-Difluorophenylhydrazine hydrochloride
o-Tolidine dihydrochloride
4-MethylMorpholine N-oxide
tert-Butyl 2-chloro-5-fluorobenzoate
PROPARGYL ACRYLATE
2-Chloro-1,3-diMethyliMidazolidiniuM hexafluorophosphate
3-Dimethylaminophenylmethylcarbinol
3-Hydrazinobenzoic acid
4-(4-Bromo-1H-pyrazol-1-yl)benzaldehyde, 95%
Lauric Acid 2-Naphthyl Ester
Hydroxyecdysone
4-Aminoguinoline
Isoflurane
Ethylenediaminetetraacetic acid tetrasodium salt
2-BroMo-M-xylene
3-Methylhexyl Acetate
tert-Butyl 4-(chloroMethyl)thiazol-2-ylcarbaMate
4-(Phenylazo)diphenylaMine, 97%
BOC-L-Methionine, 99+%
Cobalt naphthenate
9,9-Bis(4-broMophenyl)fluorene
N-(2-Ethylhexyl)-5-norbornene-2,3-dicarboximide
1,3-Bis(2,4,6-trimethylphenyl)-4,5-dihydroimidazolium chloride
4-fluoro-3-(trifluoromethyl)benzonitrile
4,6-Dichloro-2-ethoxypyrimidine
Calcium bromide
Zinc Methacrylate
4-Fluoro-4'-hydroxybenzophenone
MORPHOLINOACETONITRILE
1-(5-Nitropyridin-2-yl) 95%
1H,1H,2H,3H,3H-2-Iodoperfluoroundecan-1-ol
(Z)-4-Chloro-2-methoxycarbonylmethoxyimino-3-oxobutyric acid
Gluconic Acid Iron(II) Salt Hydrate
N-(p-Tosyl)-L-phenylalaninyl chloride
Hexyl 2-methylbutyrate
4-CHLORO-6-FLUORO-2H-BENZOPYRAN-3-CARBOXALDEHYDE
4-Amino-3-nitrophenol
1-(3-Chloropropyl)theobroMine
2-Methyl-4-(N-MethylsulfaMoyl)phenylboronic acid
Ferroceneacetic acid, 98%
N-HydroxybenzenesulfonaMide, 97%
1-Iodododecane (stabilized with Copper chip)
6-BroMo-1-hexanol
Ethyl alpha-bromophenylacetate
1-Naphthaleneacetamide
4-CHLORO-7-FLUORO-6-NITRO-QUINAZOLINE
4-Methoxy-2-(trifluoromethyl)benzaldehyde
FA ELISA Kit
IL20 ELISA Kit
Human TNF α converting enzyme,TACE ELISA Kit
GAPDH ELISA Kit
IL4 ELISA Kit
CD3 ELISA Kit
Monkey Interleukin 2,IL-2 ELISA Kit
SAMD9L ELISA Kit
Human Atrial Natriuretic Peptide,ANP ELISA Kit
ACD ELISA Kit
Rappaport-Vassiliadis Medium,Semisolid Modificatio
Modified Sulfite Agar
CORIANDER OIL
Ammonium persulfate
IMP
2-Methylpiperidine
18-Beta-Glycyrrhetinic acid
5-chloro-9-methyl-2,4,9-triazabicyclo[4.3.0]nona-2,4,7,10-tetraene
hexafluoropropene trimer
4-Amino-2-nitrophenol
1,1,2,2-Tetrafluoroethyl methyl ether
2-Fluorobenzoyl chloride
15-Pentadecanolide
3-(4-Methoxyphenyl)-1H-pyrazole-4-carboxaldehyde, 97%
n-Hexyl forMate, 97%
20(S)-Ginsenoside Rh2
Prosulfuron
Ethyl all cis-5,8,11,14,17-Eicosapentaenoate , 65%
1-NITRO-4-N-PROPYLBENZENE
3-(Cbz-AMino)-5-fluorophenylboronic acid
4-METHOXY-2-(TRIFLUOROMETHYL)BENZOIC ACID
Benzyl 2-chloro-4-(trifluoromethyl)pyrimidine-5-carboxylate 97%
Trifluoroacetyl triflate, 90%
Triethylsulfonium Bis(trifluoromethanesulfonyl)imide
5-Methoxyindole-3-acetonitrile