NCI-H735[H735]细胞系—人肺癌细胞系全国减价

NCI-H735[H735]细胞系—人肺癌细胞系全国减价 货号：GD-C16820498
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞系—将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
YtterbiuM(III) trifluoroMethanesulfonate hydrate, 98%
Pefloxacin Mesylate dihydrate
N-PROPIONYL-D-GLUCOSAMINE
3-Nitro-o-xylene
6-BroMo-(4-MethoxybenzylaMino)pyridine
4-Methoxyphenyl Mesylate
Methyl 3-fluoro-4-hydroxybenzoate
1-(3,4-Dimethylphenyl)hydrazine hydrochloride , 97%
4-Amino-3-nitrobenzophenone
Tetraamylammonium Iodide
2-Cyano-N,N-diethylacetamide
3-(1,1,2,2-TETRAFLUOROETHOXY)BENZALDEHYDE
4-Hydroxy-3-methoxybenzylamine hydrochloride
1，3-Cyclopentadiene
3,3-DiMethylacryloyl chloride
2,2'-Dipyridyl disulfide
N-(9-DiMethylaMino-1-oxononyl)-N-hydroxy-beta-alanine Methyl Ester
Methyl 2-broMothiazole-4-carboxylate
Betaine hydrochloride, 99%, extra pure
ThiMerosal, USP
Oleyl Chloride
Alpha'-Chloro-Alpha,Alpha,Alpha-trifluoro-m-xylene
1,8-Diazabicyclo[5.4.0]undec-7-ene
Copper(II) oxide
1,11-Undecanedicarboxylic acid
2,4,5-TRIFLUOROBENZYL CHLORIDE
4,4'-Diiodobiphenyl
Allylacetic acid
1-Boc-4-(p-toluenesulfonyloxyMethyl)piperidine, 96%
1-BroMo-2-naphthol, 97%
Fast Red KL Salt
5-BroMo-2-fluoro-3-Methylpyridine
3-AMinobiphenyl, HCl
N-OCTYLTRICHLOROSILANE
Fluorobenzene-d5
1-AMINOHOMOPIPERIDINE
Furfural
Trichlorovinylsilane
3,6-Dibromothieno[3,2-b]thiophene
2-Chlorophenylacetonitrile 97%
2,4,6-Trimethylbenzophenone
4-Bromo-5-fluoro-2-nitrotoluene
Cyclopentanecarboxylic acid
Boron nitride
6-(BroMoMethyl)-2-Methylquinoline
2-Fluoroanisole
Tetrahydro-2-pyriMidinone
2-(4-Methyl-1-piperazinyl)-4-pyriMidinaMine
3-(DiMethylaMino)benzyl alcohol, 97%
Ketoleucine calcium salt dihydrate
TriphenylantiMony Diacetate
Buflomedil Hydrochloride
non-1-en-1-ylboronic acid
Calcium disodium EDTA
metaphosphoric acid
Poly(sodium-p-styrenesulfonate)
4-Fluorobiphenyl-2-MethylaMine
3,4-DiMethylbenzophenone
4-HYDROXY-8-METHYL-2-(TRIFLUOROMETHYL)QUINOLINE
4-Methylpiperazinoamine dihydrochloride monohydrate , 97%
2,4-Diamino-6-(4-fluorophenyl)pyrimidine 97%
1,2-Dibromo-1-chloro-2,2-difluoroethane
(-)-Ethyl (S)-3-hydroxy-3-phenylpropionate, 98%
1-Anthraquinonesulfonic acid
Diphenylpiperidin-4-ylmethanol hydrochloride
2-(DIFLUOROMETHOXY)BENZALDEHYDE
Ethyl 5-bromonicotinate
Thioanisole
1-Chloroundecane
2-Methoxycarbonyl-3-Methylphenylboronic acid
Ethyl 2-chloroacetoacetate, 90%
6-Nitropiperonyl alcohol, 98%
Diethyl (4-BroMobenzyl)phosphonate
3,6-Bis(chloroMethyl)durene
allyl phenylacetate
D(+)-Xylose
Bexarotene
Boc-D-His(3-Bom)-OH
Porcine growth hormone releasing peptide-Ghrelin,GHRP-Ghrelin ELISA Kit
proBNP ELISA Kit
fPSA ELISA Kit
Human diamine oxidase,DAO ELISA Kit
FSH ELISA Kit
BACE 2 ELISA Kit
GFAP ELISA Kit
PKA ELISA Kit
Human fibrinopeptide A,FPA ELISA Kit
ORM ELISA Kit
Cattle Heart Broth Agar
Brain-Heart Extraction Agar Medium
MalonaMaMidine hydrochloride, 98+%
Fluorobenzene, 99%
5-(2-BroMoethyl)-2,3-Dihydrobenzofuran
2-Iodothiophene, 98%, stab. with copper
Alpha'-Chloro-Alpha,Alpha,Alpha-trifluoro-m-xylene
Ethyl 4-methylbenzeneacetate; Ethyl 4-methylphenylacetate; Ethyl p-tolylacetate;
Vitamin K
3-butylpyridine

2-FLUORO-5-METHYLPHENOL
3-Cyanomethylthiophene
Anastrozole
Hexanoyl chloride, 98%
4-Methylsalicylic acid, 99%
Alfacalcidol
1-BroMooctane
2-NAPHTHYL PHOSPHATE MONOSODIUM SALT
4,4'-Bis(2,2-diphenylvinyl)-1,1'-biphenyl
Z-GLU-OME
4-(2,4-DICHLOROPHENYL)BUT-3-EN-2-ONE
1-Fluorohexane 99%
tri-n-Butylfluorotin
mono-Ethyl malonate, 95%
6-Amino-2,3-dihydro-2,2,3,3-tetrafluoro-1,4-benzodioxine 98%