HCC-1833细胞系—人肺癌腺癌细胞系全国减价 货号:GD-C16820013
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收操作说明:请仔细阅读本操作说明及相应的细胞说明书。本公司细胞发货有四种形式:T25培养瓶(一般是贴壁细胞使用,悬浮细胞也可以)、离心管(悬浮细胞使用多,当然,有些公司也会用这种离心管形式发贴壁细胞系—但是,长期经验来说,贴壁细胞容易发生形态变化,估计是细胞培养空间狭小,血清不足导致(Z主要是怕运输延误导致这些情况发生),个人不建议用离心管形式发贴壁细胞)及干冰(冻存细胞用,主要是冬天天气冷,温度低于4℃的地方,都要考虑冻存细胞)。T25培养瓶:是用T25细胞培养瓶直接发货的形式。收到细胞后,拆开包装T25培养瓶的自封袋,不拆封口膜,表面喷75%酒精消毒后显微镜观察细胞状态(有条件可以拍下此时细胞照片)。将细胞放入37度培养箱平衡两小时以上,再处理细胞。首先将T25中培养基取出装好备用,如细胞密度高于80%,可以直接消化传代,相反则加入5-6ml培养基放回培养箱继续培养即可。离心管:是用15ml离心管发货的形式,只用于悬浮细胞发货。收到细胞后消毒处理及将细胞放入37度培养箱平衡两小时以上,再处理细胞。首先将T25中培养基取出装好备用(如果实验室没有T25培养瓶,可以暂时T75培养瓶,加入10-15ml培养基,建议10ml培养基,也可以使用T175培养瓶,加入50-60ml培养基,培养瓶越大,需要的培养基越多。当然如果刚接收到细胞密度不够的话,Z好不用大瓶子,先用小瓶子养起来再换大的,不然会长得很慢,严重的,会导致细胞死亡等危险),平衡完成后,离心(1000rpm,3min)收集细胞系—弃上清,用新的培养基重悬细胞并接种至培养瓶或皿中,放回培养箱继续培养。干冰:是用本公司冻存液冻存细胞后,直接用干冰将冻存的细胞冷冻发货的形式。收到细胞后按照细胞复苏的步骤操作即可。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
5-(4-MorpholinylMethyl)thiophene-2-boronic acid pinacol ester, 97%
3-Chloro-o-xylene
5-Chloro-2,3-diMethoxypyridine
VINYL IODIDE
2-Methoxyphenothiazine
Cyclohexen-1-ylboronic acid
Ethyl 4-(MethylaMino)piperidine-1-carboxylate
4-ethynylbenzaldehyde(SALTDATA: FREE)
4-(tert-Butyl)-2-methyl-1,3-thiazole, 97%
5-Bromo-2-methoxynicotinonitrile 98%
2,4-Dibromo-1-naphthol
Fmoc-N-methyl-L-glutamic acid 5-tert-butyl ester
2-NONYNE
1,3-Dimethyl-1H-pyrazole-5-carbonyl chloride
PANOSE
Citric acid
trans-1,3-Diphenyl-2-propen-1-ol
N-t-Butyl 3-broMo-4-MethylbenzenesulfonaMide
TetraMethylaMMoniuM acetate hydrate, 95%
Ninhydrin
Triethoxy(1H,1H,2H,2H-nonafluorohexyl)silane
ethyl linoleate
5,6-dimethoxypyrimidin-4-ylamine
n-Nonanoyl Chloride
Di-N-pentyl phthalate
(2-Aminopyridin-3-yl)methanol
2,5-DICHLOROFLUOROBENZENE
2-Chloro-N-(3-fluorobenzyl)aniline, 97%
4-MethoxybenzylaMine, 98+%
5-Chloro-2-hydroxybenzaMide, 97%
4,4'-DiMethyl-2,2'-dipyridyl
2-(Dicyclohexylphosphino)-1-(2-Methoxyphenyl)-1H-pyrrole
SILVER P-TOLUENESULFONATE
SodiuM 2,3,5,6-Tetrafluoro-4-hydroxybenzenesulfonate
2-Methoxy-1,4-naphthoquinone
1,3-DiiMinoisoindoline
4-Methyl-2-[1-(tert-butoxycarbonyl)piperid-4-yl]-1,3-thiazole-5-carboxylic acid , 97%
Phenoxyacetyl Chloride
2-(3-chlorophenoxy)-2-methylpropanoic acid(SALTDATA: FREE)
2-(2-Naphthyl)indole
Ractopamine
2,3-Dibromo-1,4-butanediol
3-Methyladipic acid
1-Phenyl-1,3-propanediol
Guanidine thiocyanate
5-BroMoacenaphthene
Ethyleneglycol-1,2-bis(succiniMidyl 3-oxypropionate)
Adenosine 3',5'-cyclic Monophosphate, 99+%
1,1-DiMethylbiguanide hydrochloride, 97%
ThioseMicarbazide
Diethylene Glycol MonoMethyl Ether Methacrylate (stabilized with MEHQ)
3,5-Dichlorophenol
4-Ethoxy-cyclohexanecarboxylic acid
4-Bromo-2-chlorotoluene
tert-butyl P,P-dimethylphosphonoacetate
3,4-Dichlorobenzyl bromide
2-Butoxy-5-fluorophenylboronic acid
t-Boc-aMinocaproic-N-hydroxysucciniMide
(4bS)-trans-8,8-TriMethyl-4b,5,6,7,8,8a,9,10-octahydro-1-isopropylphenanthren-2-ol
5-Bromo-2-fluoroaniline 98+%
Selenium(IV) sulfide, min. 94%
4-Bromo-1-methyl-1H-pyrazole
4-Chloro-2-methylacetanilide, 95+%
Calcium stearate
5-Amino-2-chlorobenzoic acid
4-(TRIFLUOROMETHYLTHIO)BROMOBENZENE
2-(7-Aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate
3-BroMopyridine
1,2-DiaMinopropane
1-Ethyl-3-Methylcyclohexane (cis- and trans- Mixture)
N'-(4-Chlorobenzoyloxy)-4-nitrobenziMidaMide
(R)-alpha-Methylphenylalanine hydrate, 98%, 98% ee
BUTYLATE
4-tert-Butylpyrocatechol
6-Methyl-2-pyridinecarboxaldehyde
Hydrogen sulfide
octadecylamine hydrochloride
3-Chlorobenzoyl chloride
VEGF ELISA Kit
CEA/CD66 ELISA Kit
Human Adiponectin,Acrp30 ELISA Kit
Human CNX ELISA Kit
VDBP ELISA Kit
Rat GATA5 (GATA Binding Protein 5) ELISA Kit
Monkey Pg (Progesterone) ELISA Kit
Human FGF2/bFGF ELISA Kit
Human thymic stromal lymphopoietin,TSLP ELISA Kit
AST ELISA Kit
RPF Agar Medium Base
Tinsdale Agar Base
Indoline
CITRATE HYDRATE
5-AMinoindane, 98+%
2-Methyl-3-pentanol, 99%
ethyl linoleate
Styrene, 1,3-butadiene polymer
dimethyl 1,2,2-trimethylcyclopentane-1,3-dicarboxylate
3-hydroxynaphthalene-2,7-disulphonic acid
2,4-DIHYDROXY-5-TERT-BUTYLBENZOPHENONE
1,1,3,3-TETRAISOPROPYLDISILOXANE
Sulfaguanidine
Cobalt(II) perchlorate hexahydrate, 99.999% (Metals basis)
Di-n-butylgerManiuM dichloride, 97%
Ethyl diphenylphosphinite
3,3-Diethoxyacrylic Acid Ethyl Ester
Theophyllol
TETRAETHYLAMMONIUM FLUORIDE TETRAHYDROFLUORIDE
EOSIN B
3-BroMo-5-Methyl-2-(3-Methoxybenzyloxy)phenylboronic acid
Disialoganglioside GD1a ,95%
Polyethyleneglycol 400 monooctylether
L-Ethionine
(1,3-Dimethyl-1H-pyrazol-4-yl)methylamine