NCI-H1573细胞系—人肺腺癌细胞系全国减价 货号:GD-C16819697
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
培养细胞真的不难,Z关键几点:1)所有的东西使用Z好的,如果你用的血清、培养皿、培养基、胰酶什么的都是进口的,真的很难相信你会把细胞养坏;2)动作要快,胰酶消化,换液什么,细胞暴露在空气中的时间越少越好。另外,要掌握好胰酶消化时间,所谓的细胞状态差,很多时候是传代的原因;3)有时间的话,需要细胞计数,传相应数目的细胞到培养皿中,可以大致清楚细胞的生长曲线,不会临时要处理,手足无措;4)要做什么心里要非常清楚,合理安排时间,传代细胞的实验穿插进行,可以节省很多时间,也不会耽误别人的实验;5)个人的实验器具自己收一套,不要和别人混用,Z近换了什么新的东西稍微记一下,试剂一旦怀疑污染,马上丢;6)传代细胞不能进去太多人,避免相互干扰。每天对待细胞比孝敬爹妈还用心,那真是捧在手里怕碎了,含在嘴里怕化了,看在眼里怕丢了,培养细胞时有哪些经验教训可以分享呢?1)严格无菌操作,多喷喷酒精,要是对灭菌的东西不放心,自己包自己送自己烘干;2)不要和别人共用试剂耗材,自己准备一套;3)有可能的话在拿到新细胞的时候做好支原体衣原体检测;4)水浴锅很脏,生化培养箱要是得手动加湿的话盘里的水也会很脏,勤换(灭菌水加进去);5)晚上离开的时候Z好给传代细胞照紫外,超净台是用完就开;6)Z好的是同一时间就你一个人在用传代细胞在养细胞。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
trans-3-Phenyl-L-proline
1-Ethylnaphthalene
α-Methyl-D-phenylalanine
7-Hydroxy-5-Methyl-1,3,4-triazaindolizine
DL-MALIC ACID DI-N-BUTYL ESTER
Phenethyl alcohol
(R)-(+)-2,2'-Bis(di-2-furanylphosphino)-6,6'-dimethoxy-1,1'-biphenyl, min. 97%
5-Amino-1-methyl-1H-indazole
Boc-(2-aminomethylphenyl)acetic acid
5-Isocyanato-1,2,3-trimethoxybenzene
Cytidine 5'-triphosphate disodium salt
D-Glutamine
Ethoxytrimethylsilane
7-Bromoindole
CalciuM chloride dihydrate
Tiglic acid
1-Ethyl-1-(M-tolyl)hydrazine
2-AcetaMido-5-fluorotoluene
5-Methyl-1-phenyl-4-(4,4,5,5-tetraMethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazole, 97%
DIBROMOACETONITRILE
2-(DiMethylaMino)vinyl 3-Pyridyl Ketone
1,2-epoxyhexane
Gum tragacanth
Hexaethyl cyclotrisiloxane
3-ethyltoluene
1-BOC-INDOLINE
3,6-Dibromocarbazole
2-Chloro-5-hydroxy-3-Methylpyridine, 97%
Methyl 2-hydroxy-3-nitrobenzoate, 98%
Quinoxaline-6-carboxylic acid, 95%
2-Chloro-N,N-diethylethylaMine hydrochloride
5-Methoxy-4-Methylindole-3-carboxaldehyde
2-Methoxy-5-sulfaMoylbenzoic acid
N,N,N',N'-Tetrakis(p-tolyl)benzidine , 98%
2-Fluoro-5-pentanoyl-1,3-phenylenediboronic acid, pinacol ester
2,2'-Azobis(2,4-diMethyl)valeronitrile
1-Benzothiophene-7-carbonitrile , 97%
3-Chloro-4-(methoxycarbonyl)benzeneboronic acid, pinacol ester 95%
1-pyrimidin-2-yl-1,4-diazepane(SALTDATA: FREE)
Benzyl L-(+)-Mandelate
4-Methylthiazole-5-carboxylic acid
PENTAMETHYLBENZENESULFONYL CHLORIDE
Nesiritide acetate
IoproMide Related CoMpound A;5-AMino-N,N'-bis(2,3-dihydroxypropyl)-2,4,6-triiodo-N-Methyl-1,3-benzenedicarboxaMide
4'-Fluoroacetophenone
N-ChloroMethyl-4-nitrophthaliMide [for HPLC Labeling]
2-Hydroxy-4-Methoxybenzonitrile
4-Nitrophenethyl broMide, 98%
3-AMino-6-chloropyridine, 98%
(S)-MEPHENYTOIN
Bis(3-ethyl-5-Methyl-4-MaleiMidophenyl)Methane
lithocholic acid
Methyl 1-Boc-4-piperidineacetate
1-(2-Fluoro-5-nitrophenyl)ethanone
ticlopidine
2-Chlorophenothiazine
7-PHENYL-1-HEPTANOL
trans-4-(4-Propylcyclohexyl)benzonitrile
1-CHLORO-3-IODOPROPANE
Potassium 3-trifluoroboratopropionate methyl ester
3-Methyl-2-benzothiazolinone hydrazone hydrochloride hydrate ,98%
Germanium tetrafluoroide
Cerium(IV) sulfate tetrahydrate, 99+%
Trimethylhydroquinone
VALERIC ACID HYDRAZIDE
TETRAVINYLSILANE
Hexyl benzoate
2,4-Dichloro-5-MethylpyriMidine
2,3,3-TriMethyl-3H-benzo[g]indole
Tri-tert-butylphosphoniuM Tetraphenylborate
2-Fluoroanisole, 98%
4-Methyl-3,4-dihydro-2H-1,4-benzoxazine-7-carbonyl chloride, 95%
Triphenylmethane
Butyl Stearate
Carbamic acid, [(1R)-1-(hydroxymethyl)propyl]-, 1,1-dimethyl...
lanthanum nitrate hydrate
3,4-Dimethoxythiophene
2-Dodecen-1-ylsuccinic anhydride, mixtureof isomers
eNOS ELISA Kit
Mouse PRKAa1 (Protein Kinase, AMP Activated Alpha 1) ELISA Kit
Human insulin-like growth factors binding protein 1,IGFBP-1 ELISA Kit
Human LMWK ELISA Kit
ADIPOR2 ELISA Kit
Rat CD44 (Cluster of Differentiation) ELISA Kit
VEGFA ELISA Kit
Human para-aminobenzoic acid,PABA ELISA Kit
IGF-1 ELISA Kit
PⅢNP ELISA Kit
Kuster’s Agar Base(Kuster, 1963)
Pseudomonas Agar for Detection of Pyocyanin
Oxalyl Dihydrazide
trans-2,3-Epoxybutane, 97%
4-Ethylphenylboronic acid
2-BenzothiazolaMine
1,2-epoxyhexane
2,3-Dichlorobenzenethiol
Phenoxyacetic Anhydride
2-cyanopropanoic acid
2-Bromochlorobenzene
(R)-(-)-6,6'-Dibromo-1,1'-bi-2-naphthol
IridiuM(IV) chloride, PreMion|r, 99.95% (Metals basis), Ir 56.5% Min
4-AMino-5-broMo-2-hydroxypyriMidine, 98%
N-FMoc-L-valine, 98%
ThuliuM(III) chloride hexahydrate
N-SucciniMidyl 6-BiotinaMidohexanoate
4-ISOPROPYLACETANILIDE
N,N-DiMethylsulfaMide
2,2-BIS(4-HYDROXY-3-ISOPROPYLPHENYL)PROPANE
3,5-Difluorobenzoyl chloride
2-Isopropyl-6-methylaniline
4-Amino-N-(2-hydroxyethyl)benzamide
5,6-dimethylfuro[2,3-d]pyrimidin-4-amine(SALTDATA: FREE)
Uramil-N,N-diacetic Acid Monohydrate