SU-DHL-16细胞系—人B淋巴瘤细胞系全国减价 货号:GD-C16820421
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
(-)-Carveol, Mixture of isoMers, 97%
FuMaric acid
3-AMinopyridine-5-boronic acid, pinacol ester
5-BroMoisatin
(R)-BINAPHANE
3,5-DibroMo-4-cyanopyridine, 97%
4-CIS-FLUORO-L-PROLINAMIDE HYDROCHLORIDE
2-Thienylmagnesium bromide, 1M solution in THF, AcroSeal
1,5,6,7-tetrahydro-2H-cyclopenta[b]pyridin-2-one(SALTDATA: FREE)
Darrow red
Nickel(II) carbonate, basic hydrate
PYRIDINE-2-THIOAMIDE
HEXAFLUOROGLUTARIC ACID
Celiprolol hydrochloride
2-AMino-2-Methyl-1-propanol
HEPES
Diisobutyl FuMarate
Methyl 3-broMoindole-5-carboxylate
ZirconiuM(IV) propoxide, ca. 70%, solution in propanol, AcroSeal
Sudan IV
Nicorandil
propyl isobutyrate
6-Chloroisatin
Tos-Arg-OH
3,5-Dimethylbenzoic acid
3-Aminopyrrolidine dihydrochloride
3-(Decyldimethylazaniumyl)propane-1-sulfonate
6-ChloropyriMidin-4(3H)-one
1-Undecanol, 98%
1-BroMo-2-chlorotetrafluoroethane, 98%
3-(4-Methoxybenzyloxy)-5-(trifluoroMethyl)benzeneboronic acid, 98%
Triethyl 2-phosphonopropionate
Ethacridine lactate Monohydrate
Octadecanoyl chloride
TRANS-1,2-BIS(TRI-N-BUTYLSTANNYL)ETHYLENE
7-(DIETHYLAMINO)COUMARIN-3-CARBOXYLIC ACID ETHYL ESTER
1,3,5-Tri-O-benzoyl-D-ribofuranose
6-Phenyl-m-anisidine hydrochloride
3-Nitrochalcone, 98%
Triallylphosphine, min. 97%
2-methyl-6-phenyl-3-pyridinecarboxylic acid ethyl ester
Benzoxazole
2-Fluoro-4-iodopyridine
4-AMINO-5-CHLORO-2-METHOXYBENZOIC ACID
2-Amino-3-hydroxypyridine
1-Benzothiophene-2-carbaldehyde
(S)-(-)-N,N-DiMethyl-1-phenylethylaMine
1-BroMo-4-(3-Methoxypropoxy)benzene
PotassiuM ethoxide, 92+%
Ethylparaben, NF
LithiuM Tri-tert-butoxyaluMinuM Hydride (ca. 30% in Tetrahydrofuran, ca. 1.0Mol/L)
D-Calcium Pantothenate
3-Methyladipic acid
2-Isonitrosoacetophenone
Trichloroethylene
5-BROMO-2-METHOXYCINNAMIC ACID
2,3,4-TriMethoxybenzoic acid
4-Methyl-1,2,4-triazole-3-thiol
4,4'-SULFONYLDIBENZOIC ACID
2-Methyl-4-acetamidopyridine ,97%
Pyridine-3-boronic acid, propane-1,3-diol cyclic ester
Ethyl 2-(2-cyanoanilino)acetate
1,3-Bis(4,5-dihydro-2-oxazolyl)benzene
2-Bromo-5-chlorophenylacetic acid
Methyldiallylamine
Xylocaine
ZIRCONIUM(IV) ETHOXIDE
8-Quinolinecarbaldehyde
1-BroMoanthracene
6-Fluoroindole-3-acetic acid
Castor oil, ethoxylated, pH-range 6.0-8.1
4-DiMethylaMino-1-naphthylisothiocyanate, 98%
(2R,3R,5S,6S)-5,6-Bis(azidoMethyl)-2,3-diMethoxy-2,3-diMethyl-1,4-dioxane
tert-Butyl 2,2,2-TrichloroacetiMidate
4-(Benzyloxy)benzyl chloride
Tasimelteon
DIMETHY-4-FLUOROPHTHALATE
3-Bromo-4-nitro-1H-indazole
CROP ELISA Kit
CACNα1E ELISA Kit
Human PCDHα7 ELISA Kit
DNM2 ELISA Kit
PAL ELISA Kit
Rat GLβ (Galactosidase, Beta) ELISA Kit
Chicken Soluble Intercellular Adhesion Molecule-1,sICAM-1 ELISA Kit
Human Elastin,ELN ELISA Kit
Human sreum albumin,HSA ELISA Kit
APT ELISA Kit
Crystal Violet Blood Agar Base
Salt Tolerance Test Medium Base
ACETYLCHOLINE IODIDE
NiobiuM(V) fluoride, 99%
5,5-DiMethylhydantoin
2-FLUORO-3-NITROBENZOIC ACID
propyl isobutyrate
ethyl 3-phenyl-1H-pyrazole-5-carboxylate
Colistimethate Sodium
1,3-Cyclohexanediol
1,2-Benzenedimethanol
5-Methoxy-2-methylbenzothiazole
DL-HoMocysteine Thiolactone Hydrochloride
ChroMiuM(III) antiMonide, 99%
PotassiuM hexanitroiridate(III), Ir 32.3% Min
Ethyl chrysantheMate, 95%
CurcuMol
4-Chloro-3-ethylphenol
2-MERCAPTO-4-METHYLPYRIMIDINE HYDROCHLORIDE
2,2-Dibutyl-[1,3,2]dioxastannolane
POTASSIUM TETRAKIS(4-CHLOROPHENYL)BORATE
4-(PHENYLAZO)DIPHENYLAMINE
1,4-Phenylenediboronic acid
Nonanoic Anhydride
4-Biphenyl Benzoate