CNE-2Z细胞系—人鼻咽癌细胞系来电咨询更多惊喜 货号:GD-C16619059
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞系—为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2,2'-Methylenebis(4-chlorophenol), 95%
1-Acetyl-4-(4-hydroxyphenyl)
SodiuM perborate tetrahydrate
(S)-(?)-Pulegone
4-Methyl-2-nitrophenylboronic acid, pinacol ester
2,4-Dichlorobenzophenone, 98%
1-Benzoylpyrrole-3-boronic acid, pinacol ester
(2S,3R)-(+)-2-Amino-3-hydroxy-4-methylpentanoic acid, 99+% ee, 99%
[2-(Dicyclohexylphosphino)ethyl]trimethylammonium chloride, min. 95%
Tricosanol, 99+%
Ethyl N-(tert-butoxycarbonyl)oxamate
1,3-DIBROMOTETRAFLUOROBENZENE
(S)-(+)-2-Methylpiperidine
1-((6-Chloro-3-pyridinyl)methyl)-N-nitro-imidazolidinimine
4-AMinoMorpholine
CyclopropylMethyl 2-chloro-4-(trifluoroMethyl)pyriMidine-5-carboxylate
2,4,6-Tris(3,4-dichlorophenyl)boroxin
Ethyl 5-broMo-1h-1,2,4-triazole-3-carboxylate
Triisopropyl borate, 98+%, AcroSeal
Prednisone, Anhydrous, Powder, USP
2-Methoxyethyl Methacrylate (stabilized with MEHQ)
2,4-Dinitrobenzoic acid
dl-2-amino-1-hexanol
2-(p-Tolyl)propionic acid
Pentadecafluorooctanoic acid
2-(1H-PYRAZOL-3-YL)PHENOL
Dimethyl acetylsuccinate
(E)-2-Nitroethenylbenzene
N-(tert-Butoxycarbonyloxy)succiniMide, 98+%
1-MethylhoMo, 97%
4-AMinophenyl disulfide
4-Chloro-4'-fluorobutyrophenone
5-(1-Chloro-2-Methylpropan-2-yl)-3-(4-nitrophenyl)-1,2,4-oxadiazole
PotassiuM (4-Cbz-aMinophenyl)trifluoroborate
4-Chloro-3-nitrophenylboronic acid
0
Ethyl Phenoxyacetate
N-(3,4-Dichlorophenyl)thiourea, 97%
Naphthalene-2-boronic acid, pinacol ester
2-amino-4-(4H-1,2,4-triazol-4-yl)benzoic acid(SALTDATA: FREE)
2,3,5-Trichlorobenzoic acid
2,6-Dimethyl-4H-pyran-4-one
Rimantadine
o-Phenetidine
TetrahexylaMMoniuM broMide
2-(2-Thienyl)-1,3-thiazole-4-carboxylic acid
4'-ForMylbenzo-18-crown 6-Ether
2-(CyclohexylaMino)nicotinonitrile
4-(2-Tetrahydro-2H-pyranoxy)phenylMagnesiuM broMide, 0.5M solution in THF, AcroSeal
Dehydroacetic acid
2-AMinobenzenesulfonic Acid
2-ethylhexyl 2,4-dichlorophenoxyacetate
Disperse Yellow 3G
Phenylmalonic acid
4,5,9,10-tetrahydropyrene
6-HEXADECANONE
Cyclooctyl isocyanate
2,2'-OXYBIS(ETHYLAMINE) DIHYDROCHLORIDE
2-(2-AMinoethylaMino)ethanol
N1-(tert-Butyl)-2-chloroacetamide
Melamine Monomer
5-Iodo-4-methyl-pyridin-2-ylamine
(8-Chloro-1-naphthylthio)acetic Acid
4-CHLORO-3-METHOXYBENZOIC ACID
P-HEXYLOXYANILINE
1-PHENYLSEMICARBAZIDE
Quercetin hydrate
2,5-Difluoroaniline
1,9-Bis(acryloyloxy)nonane (stabilized with MEHQ)
2-(CyclohexylaMino)pyriMidine-5-boronic acid, pinacol ester
Cineole, 99%
2,2-DiMethyl-4-pentenoic acid, 97%
6-Methoxy-1-tetralone
Ethyl trans-4-Decenoate
Cyclopentaneaceticacid, a-amino-
1,2,4-1H-Triazole
2-[2-[[(Phenylmethoxy)carbonyl]amino]-4-thiazolyl]-2-pentenedioic acid 5-(3-methyl-2-butenyl) ester
(R)-N-Boc-1-Naphthylalanine
ICAM2 ELISA Kit
α Manase ELISA Kit
APRIL/CD256 ELISA Kit
Human SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit
IFNα ELISA Kit
Salusin B ELISA Kit
IL6 ELISA Kit
ASP ELISA Kit
VEGFR 3 ELISA Kit
TARC ELISA Kit
Antibiotic assay medium II (low pH) (pH6.5-6.6)
Tryptose Sulfite Cycloserine Agar Base
Methyl Isobutyrate
Glycopyrrolate
5-Chloro-3-hydroxypyridine, 99%
2-Fluorophenylzinc iodide, 0.5M in THF, packaged under Argon in resealable CheMSeal^t bottles
2,4-Dinitrobenzoic acid
Direct Blue 199
Microcrystalline Cellulose
Methyl 5-fluoro-2-hydroxybenzoate
(S)-N-Boc-4-Cyanophenylalanine
Trimethylboroxine
6-Fluoroquinaldine
5-(4-Fluorophenyl)thiophene-2-carboxylic acid, 97%
3,5-Dichloro-1H-indazole, 95%
2-Ethyl-4-MethyliMidazole, 96%
4-BroMo-2-chloro-1-fluorobenzene
3,4-Difluorobenzyl broMide
SodiuM carbonate decahydrate
4-(1-BROMOETHYL)BENZOIC ACID
2-FLUOROPHENETHYLAMINE
3-BroMo-4-chlorobenzonitrile
2-Methyl-2-propen-1-ol, 98%
5-Bromo-3-chloropyridine-2-carboxylic acid
2-Phenylbutyramide, 98%