HNE-2细胞系—人鼻咽癌细胞系来电咨询更多惊喜 货号:GD-C16619255
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
6-BroMo-2-Methoxyquinoline, 96%
Styrene oxide
3,5-DIHYDROXYANISOLE HYDRATE
2-Fluoro-3-Methylpyridine-5-boronic acid
3-DiMethylaMinophenol
FradioMycin Sulfate
2-Fluoro-DL-α-phenylglycine
4-(Hydroxymethyl)tetrahydro-2H-thiopyran
Poly(L-lactic acid) [average M.W. 8000]
2-(N,N-Dimethylcarboxamido)phenylboronic acid pinacol ester
3-Morpholin-4-yl-benzoic acid ethyl ester
2-Methoxyethyl 2-[(3-nitrophenyl)methylene]acetoacetate
2-Bromo-5-(trifluoromethyl)aniline
Methyl 2-amino-3-methylbenzoate
CeriuM(III) sulfate
2-Phenoxybenzonitrile
1,3-DiMethyl-4-piperidone
Methyl 8-broMo-4-hydroxy-5-trifluoroMethylquinoline-2-carboxylate
ZirconiuM fluoride, 99.5%
Sudan Black B
4-Chloroanthranilic Acid
4-Phenyltoluene
(5E)-5-[(3-ethoxy-4-hydroxy-5-prop-2-en-1-ylphenyl)methylidene]-1-(4-ethoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
Biscyanatophenylpropane
Isonipecotic acid
3,4-DIMETHYLANISOLE
2,4-DIMETHYL-6-HYDROXYPYRIMIDINE
6-Benzothiazolol(7CI,8CI,9CI)
3'-Nitropropiophenone, 97%
3-Chlorothiophene-2-carboxylic acid, 99%
2,5-DiMethoxyphenylboronic acid
3-Methylcyclohexanol
11-Phenoxyundecanoic acid
Octadecyl acrylate
4-BROMO-2,3,5,6-TETRAFLUOROBENZOYL CHLORIDE
N,N-Diisopropyl 4-(4-BroMopyrazol-1-yl)benzenesulfonaMide
Methyl 4-amino-2-hydroxybenzenecarboxylate
Ethyl 4,4-difluoro(acetoacetate)
1-(2-chloro-6-nitrophenyl)(SALTDATA: FREE)
Cerium(III) acetylacetonate hydrate (99.9%-Ce) (REO)
(2R,3S/2S,3R)-3-(4-Chloro-5-fluoro-6-pyrimidinyl)-2-(2,4-difluorophenyl)butan-2-ol hydrochloride
Sodium diphenylamine-4-sulfonate
(Bromomethyl)cyclopropane
TEMED
3,4-DiaMinotoluene
Z-Lys(Boc)-OH
PotassiuM (4-Cyanophenyl)trifluoroborate
1,2,3,4-Butanetetracarboxylic acid, 98%
5-AMino-2-Methylbenzonitrile, 97%
Trisodium nitrilotriacetate
2,4-DiMethylthiazole
2-carboxylicacid,4-methyl-,methylester
Butoconazole
Acrylates copolymer
3,3,5,5-Tetramethylcyclohexanone
1-Adamantanecarbonyl chloride
BETA-GLYCEROL PHOSPHATE DISODIUM SALT
5-BroMo-2-(furan-2-ylMethylaMino)-3-nitropyridine
N-Benzyl-4-piperidone
2-Fluoro-4-methylphenylboronic Acid (contains varying amounts of Anhydride)
1-(2-Furyl)ethan-1-ol , 97%
Tigloyl Chloride
Zearalenone
2-Methyl-2-propyl-1,3-propanediol
Benzyl salicylate
(2-METHYLPHENOXY)ACETIC ACID
5-(2-BROMOPHENYL)-1H-TETRAZOLE
N-MethylcyclohexylaMine
1-BroMo-4-propylbenzene
3-Ethyl-2-MethylbenzothiazoliuM Iodide
Ac-asp(otbu)-oh
1-Benzothiophen-2-ylMethylaMine, 95%
COUMAPHOS
Cyclododecene (cis- and trans- Mixture)
Niclosamide ethanolamine salt
silver chromate
2-Amino-5-nitro-2'-chlorobenzophenone
Methyl 4-iodobenzoate
PRSS1 ELISA Kit
S100B ELISA Kit
Human PCDHβ1 ELISA Kit
Human dynamin 2,DNM2 ELISA Kit
NAG ELISA Kit
β3AR ELISA Kit
Chicken soluble E-selectin,sE-selectin ELISA Kit
Human BSDL ELISA Kit
BSA ELISA Kit
HBsAb ELISA Kit
Modiffied Yeast Extract-Rose Benga Broth
T1N1 Agar
DiMethyl Acetylsuccinate
NeodyMiuM(III) fluoride, anhydrous, REacton|r, 99.9% (REO)
1,2,3,4-Tetrachloro-5,5-diMethoxycyclopentadiene
3-BroMo-2-fluoro-5-Methylpyridine
4-Phenyltoluene
Phosphorous acid
cholestyramine resin
Isovaleric acid
2,6-NAPHTHALENEDICARBOXYLIC ACID
HAFNIUM CHLORIDE
2-BroMobenzenesulphonyl Chloride
1,2-Bis[(2R,5R)-2,5-diisopropyl-1-phospholanyl]ethane, 96%
Ethyl 3,6-dichloroiMidazo[1,2-a]pyridine-2-carboxylate, 95%
4′-Hexyl-4-biphenylcarbonitrile
Bis(triphenylphosphine)rhodiuM(I) carbonyl chloride
Ethyl vinyl sulfone
(R)-(+)-1-Benzylpyrrolidine-2-Methanol
Oxalic acid Monoethyl ester
1-(2-AMINOETHYL)-4-BENZYL
4,4-Dimethyl-2-oxazoline
2-Methyl-2-butenedioic acid
5-Cyano-2-fluoro-3-methoxybenzeneboronic acid, pinacol ester 95%
1,5,9-Triazacyclododecane