HKF细胞系—人成纤维细胞系来电咨询更多惊喜 货号:GD-C16618254
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤:1)选择处于对数生长期的细胞系—在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉,用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶,加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞系—使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min,10分钟);2)去上清液,加入含20%小牛血清的完全培养基,于4℃预冷15分钟后,逐滴加入已无菌的DMSO或甘油,用吸管轻轻吹打使细胞均匀,细胞浓度为5×106~1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢?答案是不一定的,具体要看培养的细胞类型来选择;3)将分装上述细胞悬液于2ml冻存管中,每管0.25ml。冻存管要将盖子盖紧,并标记好细胞名称和冻存日期,同时作好登记(日期、细胞种类及代次、冻存支数);4)将装好细胞的冻存管放到冻存盒中,-80℃冰箱过夜。;如果有程序降温器(放在-80℃冰箱过夜,放入液氮罐)Z好;或者可以在4℃,2h;然后转到-20℃,2h;-80℃,2h;放入液氮罐;5)细胞冻存在液氮中可以长期保存,但为妥善起见,冻存半年后,Z好取出一只冻存管细胞复苏培养,观察生长情况,然后再继续冻存。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
PotassiuM 4-iodophenyltrifluoroborate, 96%
DL-Proline
5-METHYLFURAN-2-CARBONYL CHLORIDE
FoMblin YR-1800
1-BROMOMETHYL-2-CHLORO-4-METHOXYBENZENE
BENZHYDRYL TRIPHENYLPHOSPHONIUM CHLORIDE
Adenosine 3'-Monophosphate Hydrate, 97%
4-Hydroxy-3-(trifluoromethyl)benzonitrile
1-Bromo-4-(methylsulphonyl)-2-nitrobenzene 98%
5-Fluoroindole-3-acetic acid
2-Chloro-4H-1,3,2-benzodioxaphosphorin-4-one
9-Bromononanoic acid
3-CHLORO-2,4-DIFLUOROBENZALDEHYDE
3-CYANO-4-FLUOROBENZENESULFONYL CHLORIDE
2-Chloro-4-iodopyridine
N-Methylpyrrolidine
1,2-DiMethoxy-4-nitrobenzene
6-BroMo-2H-isoquinolin-1-one
, 99%, extra pure
EthylenediaMinetetraacetic Acid, Iron (III) SodiuM Salt
Diphenylphosphine
O-ANISIDINE HYDROCHLORIDE
5,6-diaminopyrimidine-4-thiol
gamma-crotonolactone
Acetic acid
1,2-Dimethyl-5-nitroimidazole
4-Chlorobutyl Acetate
2-(Ethylthio)ethanol, 96%
Phenyl ^b-D-galactopyranoside, 99%
Di-n-butyltin dilaurate, 95%
3,5-Difluorobenzonitrile
2-[3-(TRIFLUOROMETHYL)PHENOXY]ETHANETHIOAMIDE
Dibutyltin dilaurate
α-Vinylbenzyl alcohol
4,5-DibroMo-1H-iMidazole
ChlorotriMethylsilane
3-(4,5-Diphenyl-1,3-oxazol-2-yl)propanoic acid, 97%
3,4,5-Triiodobenzoic acid, tech., 90%
6-Bromo-1-indanone
Calcium Alginate
Vat Green 1
METHYL 4-BOC--2-CARBOXYLATE
5-METHYL-2-(METHYLTHIO)FURAN
TRISACETATE
3-ChloropropylaMine hydrochloride
3'-Chloroindophenol SodiuM Salt [Redox Indicator]
4-(Isobutylsulfonyl)phenylboronic acid
DL-sec-Phenethyl alcohol, 97%
n-Pentyl sulfate, sodiuM salt, 99+%
Elaidic Acid
2-Chloropropionyl Chloride
3-Cyanothiophene
(R)-3-(Boc-amino)pyrrolidine
(S)-(-)-2-carboxylic acid
Erianin
3-Cyanomethylthiophene
2-(METHYLTHIO)THIAZOLE
PotassiuM 4-trifluoroboratoMethylthioMorpholine
2-(4,4-DiMethyl-4,5-dihydro-2-oxazolyl)quinoline
Iron(III) pentane-2,4-dionate
4,5-Dibromothiophene-2-carboxylic acid
N-Carbobenzoxy-L-methionine
Vanadium(IV) oxide, 99+%
2,3,5-Tribromopyridine
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide
5-(4-METHOXYPHENYL)-1H-TETRAZOLE
1,5-Naphthalene disulfonic acid
3-(1,3-Benzothiazol-2-yl)-4-Methylthiophen-2-aMine
(-)-1,4-Di-O-benzyl-L-threitol
DiMethyl 2,7-Naphthalenedicarboxylate
Ethyl 2-oxo-3,4-dihydro-1H-quinoline-3-carboxylate
Ethyl (R)-(+)-4-chloro-3-hydroxybutyrate, 98%
N-Cbz-D-Phenylalanine
Acid Fuchsine
1,2-Cyclohexanedicarboxylicacid, (1S,2S)-
6-Bromo-2,3-dihydro-4H-chromen-4-one
1,3,4,6-tetrakis(butoxymethyl)glycoluril
N-(2-Chloroethyl)acetamide
Porcine Tissue factor,TF ELISA Kit
Mouse FOXP3 (Forkhead Box Protein P3) ELISA Kit
Human tPA ELISA Kit
Human BMPR-II (Bone Morphogenetic Protein Receptor II) ELISA Kit
PⅢNP ELISA Kit
PRKAβ1 ELISA Kit
AT1R-AA ELISA Kit
Human cysteinyl leukotrienes,CysLTs ELISA Kit
Human SmIg ELISA Kit
SCL70/topo ELISA Kit
Sterile Fluid Soybean-Casein Digest Broth
Lysine Medium
Zoledronic acid
Sulfamethoxazole
5-BroMopyridine-2-Carboxylic Acid
2-Cyano-3,6-diiodopyridine, 95%
O-ANISIDINE HYDROCHLORIDE
Ethyl 4-bromocrotonate
2-bromo-6-chloropyridine
1,2-Dichloropropane
2,4-DINITROBENZYL BROMIDE
2,3,4-Trifluorophenylboronic acid
(S)-4-Benzyl-2-Oxazolidinone
2-Methyltetrahydrothiophene, 98+%
Isatin-3-oxiMe, 97%
Haloxyfop
Glycidyl (R)-(-)-4-nitrobenzenesulfonate
4-Fluorophenylglyoxal hydrate, 98%, dry wt. basis
TERBIUM(III) CARBONATE HYDRATE
(3-METHYLBENZYL)PHOSPHONIC ACID DIETHYL ESTER
CesiuM pivalate
[4-(1H-Pyrazol-1-yl)phenyl]methanol , 97%
7-(trifluoromethyl)-1,3-dihydro-2H-indol-2-one(SALTDATA: FREE)
2-(4-Methylphenyl)-1,3-thiazole-4-carboxylic acid , 97%
fmoc-l-b-Homoglutamic acid (otbu)