Walker-256细胞系—大鼠肝癌细胞系哪家提供 货号:GD-C16320580
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞系—将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Benzanilide, 98+%
3,3,4,4,5,5,6,6,7,7,8,8,8-Tridecafluorooctyl Methacrylate
4-(2-Thienyl)benzaldeyde
1-[4-(TRIFLUOROMETHYL)PHENYL]ETHANOL
TETRAETHYLGERMANE
EATON'S REAGENT
Methyl 4-Methoxysalicylate
2-Amino-thiazole-4-carboxylic acid
Methyl Hexanoate
Lanosterol
5-Ethyl-2-thiouracil ,98%
4,4'-DIPYRIDYL DISULFIDE
(R)-3,3'-DIBROMO-2,2'-DIMETHOXY-1,1'-BINAPHTHYL
LINOLENIC ACID
1-Bromo-2,6-difluorobenzene
1,4-Benzoquinone
3,5-Di-tert-butylaniline
5-BroMopyridine-2-thiol
Succinic acid, disodiuM salt hexahydrate, 97%
Mercurous Chloride, Powder, Reagent, ACS
(S)-4-Benzyloxy-1,2-butanediol
(3alpha)-3-hydroxyolean-12-en-24-oic acid
6-Chloro-3-methyluracil
adipamide
Ethylene Bis Stearamide
Dibutyl phosphate
3-Aminophenylboronic acid pinacol ester
Polyvinylpyrrolidone
Isoxazole-5-carboxylic acid, 98%
2,3,4,5,6-Pentafluorobenzyl chloride, 98%
Soda liMe
L-Buthionine-sulfoxiMine
1,2-DiMethylpropylaMine
tert-Butyl 2-broMoacrylate
N-MethylpyridiniuM-4-carboxaldehyde benzenesulfonate hydrate, 97%
POTASSIUM TRICYANOMETHANIDE
4-(2-Cyanopropan-2-yl)benzeneboronic acid 98%
2,2-Dimethylhexanoic acid 98%
Butane-1,4-diphosphonic acid
5-Bromo-4-chloro-2-(methylsulfonyl)pyrimidine ,97%
TELLURIUM TETRABROMIDE
6-CHLORONICOTINOYL CHLORIDE
4-Methoxyphenylmagnesium bromide
AMMONIUM AQUAPENTACHLORORHODATE(III)
BisMuth(III) oxide
Acetylcholine Perchlorate
2-Boc-AMino-4-chloropyridine
3-Hydroxybenzyl alcohol, 97%
Diethyl 3,4-dihydroxyfuran-2,5-dicarboxylate, 97%
5-AMINOVALERIC ACID HYDROCHLORIDE
2-Ethylhexyl Diphenyl Phosphate
Nerol
1-(Hydroxycyclohexyl)-(4-Methoxyphenyl) Acetonitrile
Adrafinil
Allyloxyphtalimide
Cyanuric acid
1,3-Dichloro-7-Methylisoquinoline
Olivetol
trans-4-Methoxy-3-buten-2-one
2-(2'-Hydroxy-3'-sec-butyl-5'-tert-butylphenyl)benzotriazole
N-(4-Bromophenyl)maleimide
2,6-Difluorobenzyl alcohol 98%
Bis(i-propylcyclopentadienyl)zirconium dichloride, 98%
3-(Cyclohexylaminocarbonyl)phenylboronic acid
4-FLUORO-2-METHYLBENZENESULFONYL CHLORIDE
AMMONIUM 2,4,6-TRINITROPHENOLATE
1,3-DIIODOBENZENE
Isophthaloyl chloride
1-(4-BroMo-2-thienyl)ethan-1-one
Diethyl (4-BroMobenzyl)phosphonate
4-VinylbenzylaMine (stabilized with MEHQ)
Trolox?, 97%
SodiuM Benzoate, Ultrapure
N-n-Octyl-3,4-thiophenedicarboxiMide
Methyl butyrate
D-fructose 1,6-diphosphate dicalcium
1-Vinylimidazole
5-Chloro-3-fluorosalicylaldehyde
OSC ELISA Kit
IL-2 ELISA Kit
Human TNFαIP2 ELISA Kit
Human GDE1 ELISA Kit
IL2 ELISA Kit
CD3 ELISA Kit
IL-2 ELISA Kit
Human Btk ELISA Kit
Human ANP ELISA Kit
ADRα1A ELISA Kit
Salmonella Arizona Agar
DTA Medium
GUM GUAIAC
AMMoniuM perrhenate(VII), 99+% (Metals basis)
5-Sulfosalicylic acid dihydrate, 98%
2-Methylnaphtho[2,3-d]oxazole, 98%
(3alpha)-3-hydroxyolean-12-en-24-oic acid
Ethyl 3-hydroxybenzoate
4-Chloro-2-fluoro-1-iodobenzene
Phthalazone
1-PHENYLNONANE
N,N-Dimethyloctylamine
2,6-DiaMino-4,5,6,7-tetrahydrobenzothiazole
PotassiuM silicate, anhydrous
2-Acetyl-7-Methoxybenzo[b]furan, 97%
2-BroMobenzyl Mercaptan
Mesityl Fluoride
1,2-DIAMINOANTHRAQUINONE
CBZ-S-Phenyl-L-cysteine
3-Nonyn-1-ol
3,4-DiMethoxyphenylboronic acid, pinacol ester
4-(2-Thienyl)butyric acid ,98%
(S,S)-(-)-1,2-Diphenyl-1,2-ethanediol, 98+%
2,6-Dichloropurine
4-Chloro-7-[(4-methylbenzene)sulfonyl]-7H-pyrrolo[2,3-d]pyrimidine