HSAS2细胞系—人皮肤成纤维细胞系哪家提供 货号:GD-C16319236
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
5-AMinoisophthalic acid, 95%
PotassiuM L-lactate solution
Copper(II) borofluoride
4,4'-DICHLOROBUTYROPHENONE
6-Benzyloxy-1h-indole-2-boronic acid
4-METHYL-1-HEPTEN-4-OL
2,6-DIBROMOBENZOIC ACID
Dichloro[(R)-(+)-2,2'-bis(diphenylphosphino)-1,1'-binaphthyl][(1R,2R)-(+)-1,2-diphenylethylenediamine]ruthenium(II), min. 90%
3-methyl-5-(2-pyrrolidinyl)isoxazole(SALTDATA: FREE)
Methyl 5-bromo-2-hydroxybenzoate
Sodium cyanoborodeuteride, 96 atom% D
3-FLUOROPYRIDINE N-OXIDE
1,2,7,8-DIEPOXYOCTANE
4-Pentyldicyclohexylanone
[3-(TrifluoroMethyl)phenyl]Methanesulfonyl chloride
3,4-Dihydro-2H-pyrido[3,2-b][1,4]oxazine
2,2'-DipyrrolylMethane
N-Methyl 4-broMo-2-fluorobenzaMide
YttriuM, 99.9%, ingot
Squalene
2-(4-Chlorophenoxy)isobutyric Acid
(R*,R*)-(+)-9,9',10,10'-tetrahydro-4,4',5,5'-tetrahydroxy-10...
Diethyl formamidomalonate
2-aminobenzophenone-2'-carboxylic acid
m-Fluoroanisole
TETRA-N-HEXYLAMMONIUM BROMIDE
Acetonitrile
N-(1-Methylethyl)-benzeneMethanaMine
1-Methyl-3-n-octyliMidazoliuM bis(trifluoroMethylsulfonyl)iMide, 99%
1,1'-Diethyl-2,2'-carbocyanine iodide, 96%
2,2'-Biphenyldicarboxylic Acid
2,2-DiMethyl-6,6,7,7,8,8,8-heptafluoro-3,5-octanedione
1,6-Naphthalenedisulfonic acid disodiuM salt
O-tert-Butyl-L-tyrosine Methyl ester hydrochloride
5-FLUORO-2-METHYLPHENYLTHIOUREA
2-Methyl-4-(1,1,1,2,3,3,3-heptafluoro-2-propyl)aniline
Strontium sulfide (99.9%-Sr)
9-Ethylcarbazole-3-carboxaldehyde N-Methyl-N-phenylhydrazone
3-bromo-2,6-pyridinediamine(SALTDATA: FREE)
Benzyl Diethyldithiocarbamate
5-(2-PYRIDYL)THIOPHENE-2-CARBOXYLIC ACID
N,N'-Dimorpholinomethane
Phenoxybenzamine hydrochloride
Sodium sulfite
2,4-DiMethylphenol
2-Methoxy-4-Methyl-6-(MethylaMino)-1,3,5-triazine
1,1,1,2-Tetrachloroethane (1Mg/ML in Methanol) [for Water Analysis]
1-BroMopinacolone, 93%
5-(BroMoMethyl)-2,1,3-benzoxadiazole, 97%
D-ASPARTIC ACID(OBZL)-OBZL P-TOSYLATE
2-Mercaptopyridine N-Oxide SodiuM Salt (40% in Water, ca. 3.3Mol/L)
Gemeprost
Atractylenolide-1
Poly(antimony ethylene glycoxide)
Z-TYR-OTBU H2O
1-(2-BROMOETHOXY-4-BROMOBENZENE)
3-Chloro-2-Methylpropionitrile
6,7-Methylenedioxy-4-Methyl-3-MaleiMidocouMarin [for HPLC Labeling], 98%
2,2,3,3-Tetrafluoropropyl Acrylate (stabilized with MEHQ)
3-Acetyl-6-bromo-2H-chromen-2-one , 97%
3-(n-Amyl)-2,4-pentanedione
3-Amino-N-cyclopropylbenzenesulphonamide 97%
D-Arabinose-5-phosphate disodium salt
4-CHLORO-6-METHYL-2-(TRIFLUOROMETHYL)QUINOLINE
5-NITRO-2-FUROYL CHLORIDE
SUCROSE MONOLAURATE
ALLYL N-PROPYL SULFIDE
PotassiuM diphosphate
1-BroMo-3-chloropropane
(3R)-(+)-3-(EthylaMino)pyrrolidine
4'-Chlorobiphenyl-3-carboxylic acid
2-Fluoro-5-Methoxyphenylboronic acid, 95%
CARBOFURAN
Triarachidin
4-Methoxypyridine-N-oxide
Mercury(II) sulfate
1,3,5-trimethylcyclohexane
2,6-Dichloro-4-nitrophenol
FGF1 ELISA Kit
S100A7 ELISA Kit
Human TPM2 ELISA Kit
Human UQCRC1 ELISA Kit
α-KGDHC ELISA Kit
Rat β2-glycoprotein 1 antibody IgA/G/M,β2-GP1 IgA/G/M ELISA Kit
CAV1 ELISA Kit
Human cholecystokinin,CCK ELISA Kit
Human plasma anticoagulant protein S,PS ELISA Kit
PANDER ELISA Kit
T1N1 Agar
Triple Sugar Iron Agar
Diethyl 2-acetylglutarate, 98+%
CeriuM Fluoride
5,6,7,8-Tetrahydroquinoxaline, 99%
2-Fluoro-4-iodobenzeneboronic acid, 98%
(R*,R*)-(+)-9,9',10,10'-tetrahydro-4,4',5,5'-tetrahydroxy-10...
Lithium molybdate
endo-3-Bromo-D-camphor
(R)-4-hydroxy-α-methyl-Benzenepropanol
2,4-Dimethylimidazole
3,4-DIMETHYLANISOLE
(S)-2-AMino-3-Methyl-1-Butanol
GadoliniuM(III) chloride hexahydrate, REacton|r, 99.99% (REO)
1-Boc-3-(MethylaMino)azetidine hydrochloride, 95%
2-Chlorocyclohexanone
FenpyroxiMate
4-Hydroxybenzoic acid
2,2'-(1,2-Ethenediyldi-4,1-phenylene)bisbenzoxazole
2-Pentanol
Pentafluorobenzene
2-Chloroaniline hydrochloride
3-Chlorothietane-1,1-dioxide
4-(4-alpha-Cumylphenoxy)phthalonitrile
N-Ethylsuccinimide