HFB细胞系—人皮肤成纤维细胞系哪家提供 货号:GD-C16318255
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞冻存的步骤:1)选择处于对数生长期的细胞系—在冻存前一天Z好换液。将多个培养瓶中的细胞培养液去掉,用0.25% 胰蛋白酶消化。适时去掉胰蛋白酶,加入少量新培养液。用吸管吸取培养液反复吹打瓶壁上的细胞系—使其成为均匀分散的细胞悬液。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min,10分钟);2)去上清液,加入含20%小牛血清的完全培养基,于4℃预冷15分钟后,逐滴加入已无菌的DMSO或甘油,用吸管轻轻吹打使细胞均匀,细胞浓度为5×106~1×107/mL之间。(冻存培养液的配置是不是一定要用小牛血清呢?答案是不一定的,具体要看培养的细胞类型来选择;3)将分装上述细胞悬液于2ml冻存管中,每管0.25ml。冻存管要将盖子盖紧,并标记好细胞名称和冻存日期,同时作好登记(日期、细胞种类及代次、冻存支数);4)将装好细胞的冻存管放到冻存盒中,-80℃冰箱过夜。;如果有程序降温器(放在-80℃冰箱过夜,放入液氮罐)Z好;或者可以在4℃,2h;然后转到-20℃,2h;-80℃,2h;放入液氮罐;5)细胞冻存在液氮中可以长期保存,但为妥善起见,冻存半年后,Z好取出一只冻存管细胞复苏培养,观察生长情况,然后再继续冻存。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-Nitroanisole, 99%
5-Chloroindole
TETRADECYLBENZENE
7,8-DihydroxychroMen-2-one
4-DIETHYLAMINO-2-BUTYN-1-OL
3-Hydroxy-2-(hydroxyMethyl)pyridine hydrochloride
4-Nitro-3-picoline N-oxide
Butyl Carbamate
DL-(3-Chlorophenyl)glycine
2,2-Dimethoxy-1-thia-2-silacyclopentane
Salicylic Acid p-Tolyl Ester
METHYL 2-METHOXY-5-NITROBENZOATE
3-(PERFLUOROBUTYL)PROPANOL
Tamoxifen
2,4-Bis(trifluoromethyl)phenylboronic acid
1,10-DibroModecane
2,2-DiMethylthiazolidine
4-BroMo-isothiazole
-2-one, 95%
Ethylxanthic Acid PotassiuM Salt
Diethyl (2-Oxo-2-phenylethyl)phosphonate
8-hydroxyquinolinium citrate
zinc malate
2-Oxazolidone
Tetramethylammonium bromide
1-(2-HYDROXY-5-METHYLPHENYL)-3-PHENYL-1,3-PROPANEDIONE
7-Bromoindole
4-AMino-2,6-DichloropyriMidine
4-(4-Fluorobenzyloxy)benzyl alcohol, 98%
3-BroMo-4-hydroxypyridine, 97%
3-(2-Furyl)propionic acid, 97%
4-AMinotetrahydropyran
Thiophene-2-boronic acid, neopentyl glycol ester
α-Methoxy-α-(trifluoroMethyl)phenylacetic acid
3-BroMo-1-(triisopropylsilyl)pyrrole , 95%
Acetone O-(benzyloxycarbonyl)oxiMe
MagnesiuM
2-Chloro-6-iodoaniline
5-Bromo-4-chloro-3-indolyl sulfate potassium salt, 99%
Bis [3-(triethoxysilyl )propyl ] tetrasulphide
4,5-Diamino-6-mercaptopyrimidine ,97%
ALUMINUM ETHOXIDE
NAPHTHALENE-2-CARBOXAMIDE
Potassium heptadecafluoro-1-octanesulfonate
4-Chloro-3-fluorobenzaldehyde
2-Chloro-5-fluorotoluene
Diethyl PhosphoraMidate
4-(4-BroMophenylsulfonyl)Morpholine
SodiuM peroxide, 96%
L-Tyrosyl-L-phenylalanine
2'-Deoxy-5-Methylcytidine
1,1,1,3,3-Pentafluoropropane
1H-pyrazole-1-carboxamidine monohydro-chloride
Dimethyl itaconate
2-Picoline
TRIETHYLSULFONIUM IODIDE
DIPHENYL TEREPHTHALATE
2-(4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLAN-2-YL)PHENOL
VINYL CINNAMATE
Bis(trimethylsiloxy)cyclobutene
2-Methyl-1,3-benzoxazol-5-amine
{[4-(Trifluoromethyl)phenyl]thio}acetic acid 97%
Tripropylene Glycol (mixture of isomers)
Triphosgene
STANNOUS CHLORIDE
Methyl 2,4,6-trimethylbenzoate
3'-Bromoacetophenone
1-(1,3-Benzodioxol-5-yl)-2-broMoethan-1-one
(-)-10-CaMphorsulfoniMine
E-Penten-1-ylboronic acid
Guanosine, 99%
3,5-DibroMopyridine-N-oxide, 97%
Ethoxydiphenylphosphine
1-Phenylnaphthalene
Basic Violet 4
Chlorzoxazone
2-Methyl-2-adamantylmethacrylate
(S)-Methyl oxazaborolidine
Porcine Tissue factor,TF ELISA Kit
FOXP3 ELISA Kit
Human Tissue-type Plasiminogen Actilyse,t-PA ELISA Kit
Human BMPR-II (Bone Morphogenetic Protein Receptor II) ELISA Kit
PⅢNT ELISA Kit
CD40L ELISA Kit
AT-2 ELISA Kit
Human cysteinyl leukotrienes,CysLTs ELISA Kit
Human CYGB ELISA Kit
AAHA ELISA Kit
Soybean-Casein Digest Agar Medium
Lysine Medium
ZolMitriptan
Sulfosulfuron
5-BROMO-2-PHENOXYPYRIMIDINE
Methyl 2-Cyano-3,3-DI(MethylTHIO)Acrylate
8-hydroxyquinolinium citrate
2,2-dimethyl-propanedioic acid dimethyl ester
Light Green SF
2,3-Dichloro-1-propene
4-BENZOYLBUTYRIC ACID
HOLO-TRANSFERRIN
5-AMinoquinoline
Choline iodide, 98%
5-Nitroacenaphthene, 98%
Ethylene iMine polyMer
(±)-3-AMino-1,2-propanediol
2,2'-Dipyridyl
4'-(AMYLOXY)BENZYLIDENE-4-CYANOANILINE
3-CHLORO-2,4,5,6-TETRAFLUOROBENZOIC ACID
2,4-Difluoroaniline
3-(Aminomethyl)phenol
1H,1H,2H-Perfluorotetradec-1-ene 97%
4-(4-methyl-4H-1,2,4-triazol-3-yl)aniline(SALTDATA: FREE)
N-(tert-Butyl)benzenesulphonamide